DOI QR코드

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An Improved Total RNA Extraction Method for White Jelly Mushroom Tremella fuciformis Rich in Polysaccharides

  • Zhu, Hanyu (College of Food Science and Technology, Huazhong Agricultural University) ;
  • Sun, Xueyan (College of Food Science and Technology, Huazhong Agricultural University) ;
  • Liu, Dongmei (College of Food Science and Technology, Huazhong Agricultural University) ;
  • Zheng, Liesheng (College of Plant Science and Technology, Huazhong Agricultural University) ;
  • Chen, Liguo (College of Plant Science and Technology, Huazhong Agricultural University) ;
  • Ma, Aimin (College of Food Science and Technology, Huazhong Agricultural University)
  • 투고 : 2017.09.11
  • 심사 : 2017.11.30
  • 발행 : 2017.12.01

초록

An improved method for extracting high quality and quantity RNA from a jelly mushroom and a dimorphic fungus-Tremella fuciformis which is especially rich in polysaccharides, is described. RNA was extracted from T. fuciformis mycelium M1332 and its parental monokaryotic yeast-like cells Y13 and Y32. The A260/280 and A260/230 ratios were both approximately 2, and the RNA integrity number was larger than 8.9. The yields of RNA were between 108 and $213{\mu}g/g$ fresh wt. Downstream molecular applications including reverse transcriptional PCR and quantitative real-time PCR were also performed. This protocol is reliable and may be widely applicable for total RNA extraction from other jelly mushrooms or filamentous fungi rich in polysaccharides.

키워드

참고문헌

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