Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지
DOI QR코드

DOI QR Code

Association of the Genotypes of Single Nucleotide Polymorphism Marker rs81437607 with Capric Acid Contents in longissimus dorsi Muscle in Pigs

돼지 등심 내 카프르산 함량과 단일염기다형 마커 rs81437607 유전자형의 상관

  • Kim, Sang-Geum (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA) ;
  • Park, Hee-Bok (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA) ;
  • Kang, Yong-Jun (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA) ;
  • Shin, Hyunsook (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA) ;
  • Cho, Sang-Rae (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Lee, Wang-Shik (Faculty of Biotechnology, Jeju National University) ;
  • Han, Sang-Hyun (Educational Science Research Institute, Jeju National University) ;
  • Cho, In-Cheol (Subtropical Livestock Research Institute, National Institute of Animal Science, RDA)
  • 김상금 (농촌진흥청 국립축산과학원 난지축산연구소) ;
  • 박희복 (농촌진흥청 국립축산과학원 난지축산연구소) ;
  • 강용준 (농촌진흥청 국립축산과학원 난지축산연구소) ;
  • 신현숙 (농촌진흥청 국립축산과학원 난지축산연구소) ;
  • 조상래 ;
  • 이왕식 (제주대학교 생명공학부) ;
  • 한상현 (제주대학교 교육과학연구소) ;
  • 조인철 (농촌진흥청 국립축산과학원 난지축산연구소)
  • Received : 2016.09.09
  • Accepted : 2016.09.29
  • Published : 2016.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study tested the association between genotypes of the single nucleotide polymorphism (SNP) marker, rs81437607 and capric acid (FA_C10_0) compositions in longissimus dorsi muscle in pigs. Eighteen fatty acid (FA) compositions were measured in a total of 974 $F_2$ animals among 1,106 $F_2$ progeny produced between Landrace and Jeju Black Pig (JBP). Among FA compositions tested, we identified a cluster of highly significant SNPs for capric acid compositions on 58 Mb position of Sus scrofa chromosome 12 (SSC12) using genome-wide association study (GWAS) with $F_2$ genotypes from SNP panel analysis. GWAS results showed that the rs81437607 was the highest trait-related SNP marker with capric acid levels. Three genotypes (C/C, C/T and T/T) of rs81437607 marker were found in $F_2$ population by further pyrosequencing. Association analysis results showed the significant differences between rs81437607 genotypes and capric acid compositions (P<0.05). The $F_2$ pigs harboring rs81437607 C/C ($0.119{\pm}0.002%$) and C/T ($0.116{\pm}0.002%$) genotypes showed additively higher levels of capric acid content than those of T/T homozygotes ($0.109{\pm}0.002%$) ($P=1.30{\times}10^{-12}$). These results suggested that the genetic variations of rs81437607 may be helpful to find causative variants and assist as molecular genetic markers for improving the capric acid contents in longissimus dorsi muscle in pigs.

Keywords

References

  1. Abe T, Hirasaka K, Kohno S, Tomida C, Haruna M, Uchida T, Ohno A, Oarada M, Teshima-Kondo S, Okumura Y, Choi I, Aoyama T, Terao J and Nikawa T. 2016. Capric acid up-regulates UCP3 expression without PDK4 induction in mouse C2C12 myotubes. J. Nutr. Sci. Vitaminol. 62:32-39. https://doi.org/10.3177/jnsv.62.32
  2. Babayan VK. 1987. Medium chain triglycerides and structured lipids. Lipids. 22:417-420. https://doi.org/10.1007/BF02537271
  3. Ballester M, Revilla M, Puig-Oliveras A, Marchesi JA, Castello A, Corominas J, Fernandez AI and Folch JM. 2016. Analysis of the porcine APOA2 gene expression in liver, polymorphism identification and association with fatty acid composition traits. Anim. Genet. In press
  4. Bartz M, Szydlowski M, Kociucka B, Salamon S, Jelen HH and Switonski M. 2013. Transcript abundance of the pig stearoyl-CoA desaturase gene has no effect on fatty acid composition in muscle and fat tissues, but its polymorphism within the putative microRNA target site is associated with daily body weight gain and feed conversion ratio. J. Anim. Sci. 91:10-19. https://doi.org/10.2527/jas.2012-5380
  5. Benitez R, Nunez Y, Fernandez A, Isabel B, Fernandez AI, Rodriguez C, Barragan C, Martin-Palomino P, Lopez-Bote C, Silio L and Ovilo C. 2015. Effects of dietary fat saturation on fatty acid composition and gene transcription in different tissues of Iberian pigs. Meat Sci. 102:59-68. https://doi.org/10.1016/j.meatsci.2014.12.005
  6. Birren B, Green ED, Klapholz S, Myers RM and Roskams J. 1997. Genome analysis: a laboratory manual. Cold Spring Harbor Laboratory Press, New York.
  7. Cho IC, Han SH, Fang M, Lee SS, Ko MS, Lee H, Lim HT, Yoo CK, Lee JH and Jeon JT. 2009. The robust phylogeny of Korean wild boar (Sus scrofa coreanus) using partial D-loop sequence of mtDNA. Mol. Cells. 28:423-430. https://doi.org/10.1007/s10059-009-0139-3
  8. Choi YS. 2004. Studies on the pork quality of Korean native black pigs and its improvement through dietary manipulation. Ph. D. thesis. Kangwon National Univ. Chuncheon, Korea. pp. 1-169.
  9. Dekkers JC and Hospital F. 2002. The use of molecular genetics in the improvement of agricultural populations. Nat. Rev. Genet. 3:22-32. https://doi.org/10.1038/nrg701
  10. Desbois AP and Smith VJ. 2010. Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential. Appl. Microbiol. Biotechnol. 85:1629-1642. https://doi.org/10.1007/s00253-009-2355-3
  11. Estany J, Ros-Freixedes R, Tor M and Pena RN. 2014. A functional variant in the stearoyl-CoA desaturase gene promoter enhances fatty acid desaturation in pork. PLoS One. 9:e86177. https://doi.org/10.1371/journal.pone.0086177
  12. Folch J, Lees M and Stanley GHS. 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 226:497-509.
  13. Han SH, Shin KY, Lee SS, Ko MS, Oh HS and Cho IC. 2012. Porcine SPP1 gene polymorphism association with phenotypic traits in the Landrace $\times$ Jeju (Korea) Black pig F2 population. Mol. Biol. Rep. 39:7705-7709. https://doi.org/10.1007/s11033-012-1606-z
  14. Hanczakowska E, Szewczyk A, Swiatkiewicz M and Okon K. 2013. Short- and medium-chain fatty acids as a feed supplement for weaning and nursery pigs. Pol. J. Vet. Sci. 16:647-654.
  15. Hayama K, Takahashi M, Yui S and Abe S. 2015. Inhibitory effects of several saturated fatty acids and their related fatty alcohols on the growth of Candida albicans. Drug Discov. Ther. 9:386-390. https://doi.org/10.5582/ddt.2015.01062
  16. Huang WC, Tsai TH, Chuang LT, Li YY, Zouboulis CC and Tsai PJ. 2014. Anti-bacterial and anti-inflammatory properties of capric acid against Propionibacterium acnes: a comparative study with lauric acid. J. Dermatol. Sci. 73:232-240. https://doi.org/10.1016/j.jdermsci.2013.10.010
  17. Kalinowski ST, Taper ML and Marshall TC. 2007. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol. Ecol. 16:1099-1106. https://doi.org/10.1111/j.1365-294X.2007.03089.x
  18. Kanabus M, Fassone E, Hughes SD, Bilooei SF, Rutherford T, Donnell MO, Heales SJ and Rahman S. 2016. The pleiotropic effects of decanoic acid treatment on mitochondrial function in fibroblasts from patients with complex I deficient Leigh syndrome. J. Inherit. Metab. Dis. 39:415-426. https://doi.org/10.1007/s10545-016-9930-4
  19. Kim JH, Han SH, Kang MC, Oh JH, Jung YH, Kim GO and Oh MY. 2006. Ancient pigs on Jeju Island, Korea: molecular identification and phylogenetic relationship with extant native pigs. Genes Genomics. 28:385-393.
  20. Maharani D, Park HB, Lee JB, Yoo CK, Lim HT, Han SH, Lee SS, Ko MS, Cho IC and Lee JH. 2013. Association of the gene encoding stearoyl-CoA desaturase (SCD) with fatty acid composition in an intercross population between Landrace and Korean native pigs. Mol. Biol. Rep. 40:73-80. https://doi.org/10.1007/s11033-012-2014-0
  21. Morrison W and Smith LM. 1964. Preparation of fatty acids methyl ester and dimethylacetals from lipids with boron fluoride-methanol. J. Lipid Res. 5:600-608.
  22. Nagao K and Yanagita T. 2010. Medium-chain fatty acids: Functional lipids for the prevention and treatment of the metabolic syndrome. Pharmacol. Res. 61:208-212. https://doi.org/10.1016/j.phrs.2009.11.007
  23. Park EJ, Kim SA, Choi YM, Kwon HK, Shim W, Lee G and Choi S. 2011. Capric acid inhibits NO production and STAT3 activation during LPS-induced osteoclastogenesis. PLoS One. 6: e27739. https://doi.org/10.1371/journal.pone.0027739
  24. Purcell S, Neale B, Todd-Brown K, Thomas L, Ferreira MA, Bender D, Maller J, Sklar P, de Bakker PI, Daly MJ and Sham PC. 2007. PLINK: a tool set for whole-genome association and population-based linkage analyses. Am. J. Hum. Genet. 81:559-575. https://doi.org/10.1086/519795
  25. Revilla M, Ramayo-Caldas Y, Castello A, Corominas J, Puig-Oliveras A, Ibanez-Escriche N, Munoz M, Ballester M and Folch JM. 2014. New insight into the SSC8 genetic determination of fatty acid composition in pigs. Genet. Sel. Evol. 46:28. https://doi.org/10.1186/1297-9686-46-28
  26. Rothschild MF and Soller M. 1997. Candidate gene analysis to detect genes controlling traits economic importance in domestic livestock. Probe. 8:13-20.
  27. Spangler ML, Bertrand JK and Rekaya R. 2007. Combining genetic test information and correlated phenotypic records for breeding value estimation. J. Anim. Sci. 85:641-649. https://doi.org/10.2527/jas.2006-617
  28. Sprong RC, Hulstein MFX and Meer RV. 2001. Bactericidal activities of milk lipids. Antimicrob. Agents Chemother. 45:1298-1301. https://doi.org/10.1128/AAC.45.4.1298-1301.2001
  29. Stachowiak M, Szydlowski M, Flisikowski K, Flisikowska T, Bartz M, Schnieke A and Switonski M. 2014. Polymorphism in 3' untranslated region of the pig PPARA gene influences its transcript level and is associated with adipose tissue accumulation. J. Anim. Sci. 92:2363-2371. https://doi.org/10.2527/jas.2013-7509
  30. Takeuchi H, Sekine S, Kojima K and Aoyama T. 2008. The application of medium-chain fatty acids: edible oil with a suppressing effect on body fat accumulation. Asia Pac. J. Clin. Nutr. 17:320-323.
  31. Thevenet J, De Marchi U, Domingo JS, Christinat N, Bultot L, Lefebvre G, Sakamoto K, Descombes P, Masoodi M and Wiederkehr A. 2016. Medium-chain fatty acids inhibit mitochondrial metabolism in astrocytes promoting astrocyteneuron lactate and ketone body shuttle systems. FASEB J. 30:1913-1926. https://doi.org/10.1096/fj.201500182
  32. Wang Z, Li Q, Chamba Y, Zhang, Shang P, Zhang H and Wu C. 2015. Identification of genes related to growth and lipid deposition from transcriptome profiles of pig muscle tissue. PLoS One. 10:e0141138. https://doi.org/10.1371/journal.pone.0141138
  33. Wojtysiak D and Kaczor U. 2011. Effect of g.2728g>A and g.3996t>C polymorphisms at the leptin gene locus on microstructure and physicochemical properties of longissimus lumborum muscle of Polish Landrace pigs. Folia Biol. 59:77-82. https://doi.org/10.3409/fb59_1-2.77-82
  34. Wu WH, Lin BY, Kuo YH and Huang CJ. 2009. Triglycerides constituted of short and medium chain fatty acids and dicarboxylic acids in Momordica charantia, as well as capric acid, inhibit PGE2 production in RAW264.7 macrophages. Food Chem. 117: 306-311. https://doi.org/10.1016/j.foodchem.2009.04.004
  35. Yang B, Zhang W, Zhang Z, Fan Y, Xie X, Ai H, Ma J, Xiao S, Huang L and Ren J. 2013. Genome-wide association analyses for fatty acid composition in porcine muscle and abdominal fat tissues. PLoS One. 8:e65554. https://doi.org/10.1371/journal.pone.0065554
  36. Zentek J, Ferrara F, Pieper R, Tedin L, Meyer W and Vahjen W. 2013. Effects of dietary combinations of organic acids and medium chain fatty acids on the gastrointestinal microbial ecology and bacterial metabolites in the digestive tract of weaning piglets. J. Anim. Sci. 91:3200-3210. https://doi.org/10.2527/jas.2012-5673
  37. Zhang W, Zhang J, Cui L, Ma J, Chen C, Ai H, Xie X, Li L, Xiao S, Huang L, Ren J and Yang B. 2016. Genetic architecture of fatty acid composition in the longissimus dorsi muscle revealed by genome-wide association studies on diverse pig populations. Genet. Sel. Evol. 48:5. https://doi.org/10.1186/s12711-016-0184-2
  38. 김훈, 김진우, 박새롬, 이영섭, 홍민욱, 이승규, 이경수, 원정일, 이정구, 이성기, 이학교, 이성진. 2013. 재래돼지에서 6개 SNP와 경제형질, 고기품질 간의 연관성 분석. 동물자원연구. 24:8-15.
  39. 김명직, 조규호, 김두완, 최종환, 최성복, 김재환, 변미정. 2012. 한국 재래돼지의 산육능력 향상을 위한 부계 손발지수 개발에 관한 연구. 동물자원연구. 23:84-88.
  40. 노경희, 이기종, 박종석, 김현욱, 이경렬, 김종범, 2010. 유채에서의 중쇄지방산 생산. 한국응용생명화학회지. 53:65-70.
  41. 손영곤, 김두완, 이승수. 2013. 포유기 장쇄지방산과 중쇄지방산의 급여가 이유자돈의 증체와 균일성에 미치는 영향. 농업생명과학연구. 47:203-209.
  42. 신원집, 정진우, 최광수, 신수길. 2000. 돼지에 있어서 생식기관액 중 지방산 조성과 조성율. 한국수정란이식학회지. 15:137-145.
  43. 윤태헌, 임경자, 김을상, 정우감. 1982. 인유 및 우유의 지방산 조성에 관하여. 한국영양식량학회지. 11:15-20.