Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Developmental and survivability according to cryopreservation of in vitro produced bovine embryos cultured by addition of Antioxident cysteine

Cysteine 첨가로 배양된 소 수정란의 발달과 동결성 효과

  • 조상래 (농촌진흥청국립축산과학원) ;
  • 강성식 (농촌진흥청국립축산과학원) ;
  • 김의형 (농촌진흥청국립축산과학원) ;
  • 김시동 (농촌진흥청국립축산과학원) ;
  • 이석동 (농촌진흥청국립축산과학원) ;
  • 전기준 (농촌진흥청국립축산과학원) ;
  • 박창석 (예천군농업기술센터) ;
  • 양병철 (농촌진흥청국립축산과학원)
  • Received : 2016.09.20
  • Accepted : 2016.09.30
  • Published : 2016.09.30
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Abstract

The aim of the present study was to assess the embryo development and survivability of post-thawed bovine embryos produced in vitro by addition of cysteine. The rates of metaphase II formation were not differed significantly among three groups(TCM199 73.8%, TCM199 with 0.3% cysteine 76.9%, TCM199 with 0.5% cysteine 83.8%, respectively). No difference of cleavage rate(70.6~74.6%) was seen among three culture medium(TCM199 70.6%, CR1aa 71.3%, SOF 74.6%) with 0.5M cysteine. however, Significantly(P<0.05) higher development rate into blastocyst stage by 0.5M cysteine addition was obtained in SOF medium(35.6%) than in TCM199(27.6%) or CR1aa(26.6%), however no significant differences in the cleavage rates were among three culture medium. After frozen the blastocysts cultured with 0.5M cysteine, The re-expansion rates were 61.3%~86.4% among groups, and hatching rates were 26.3%~46.9% among groups, the rates of re-expansion and hatching were significantly(P<0.05) higher in SOF medium(86.4% and 46.9%) than those in TCM199(61.3% and 26.3%) and CR1aa medium(87.1 and 44.4%). After thawing, the blastocyst re-expansion rate was significantly(P<0.05) higher in in vivo (87.1%) and in vitro (70.3%) embryos. In conclusion, our results demonstrate that supplementation of IVM and IVC medium with 0.5M cysteine improved the quality of in vitro production embryo and post- thawed embryo. Future studies comparing these media systems in well-designed trials should be performed.

Keywords

References

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