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Effect of glutathione on tetraploid embryo development in the pigs

  • Kim, Hwa-Young (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Sang-Hee (College of Animal Life Sciences, Kangwon National University) ;
  • Hwangbo, Yong (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Seung Tae (College of Animal Life Sciences, Kangwon National University) ;
  • Lee, Eunsong (College of Veterinary Medicine, Kangwon National University) ;
  • Park, Choon-Keun (College of Animal Life Sciences, Kangwon National University)
  • Received : 2016.08.19
  • Accepted : 2016.09.12
  • Published : 2016.09.30

Abstract

The objective of this study was to investigate to influence of glutathione (GSH) on development and antioxidant enzyme activity in tetraploid porcine embryos. Tetraploid embryos were produced using parthenogenetic 2-cell embryo by electrofusion method. Tetraploid embryo development was observed every 24 hours and intracellular antioxidant enzyme activity was measured at 120 hours after electrofusion. The 4-cell to 16-cell stage tetraploid embryos was increased in 100 and $500{\mu}M$ GSH-treated groups compared control group at 48 hours (P < 0.05) but cleavage rates were not significantly different among the GSH treatment groups at 48, 72, 96, and 120 hours. Blastocyst formation was significantly increased by 300 and $500{\mu}M$ GSH at 120 hours in tetraploid embryos (P < 0.05). But blastocyst cell number were not significantly different among the GSH treatment groups ($16.4{\pm}0.8$, $16.8{\pm}2.6$, $18.5{\pm}2.8$ and $17.5{\pm}1.8$). The intracellular antioxidant enzyme level was increased in $500{\mu}M$ GSH compared to 0 and $100{\mu}M$ GSH (P < 0.05). We suggest that GSH may be improve development of tetraploid embryo in pigs.

Keywords

Acknowledgement

Supported by : Food and Rural Affairs, Ministry of Agriculture

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