한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제31권3호
  • /
  • Pages.185-190
  • /
  • 2016
  • /
  • 2508-755X(pISSN)
  • /
  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
권호 표지
DOI QR코드

DOI QR Code

소 난소 저온 보존이 난자의 체외 발달에 미치는 영향

Low temperature preservation of bovine ovaries on in vitro development of oocytes

  • 김성우 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김민수 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김찬란 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김동교 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 김남태 (농촌진흥청 국립축산과학원 가축유전자원센터) ;
  • 성환후 (농촌진흥청 국립축산과학원 가축유전자원센터)
  • Kim, Sung Woo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Min Su (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Chan-Lan (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Dongkyo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Kim, Namtae (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA) ;
  • Seong, Hwan-Hoo (Animal Genetic Resources Research Center, National Institute of Animal Science, RDA)
  • 투고 : 2016.07.18
  • 심사 : 2016.09.29
  • 발행 : 2016.09.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

During the ovary preservation in low temperature, the cumulus oocyte complexes(COCs) lose their developmental competences after in vitro fertilization. We used phosphate-buffered saline (PBS) as a basic solutions of at various temperatures (25, 15 or $5^{\circ}C$) and supplemented them with 1mM glucose and 0.5mM glutamine as a source of carbohydrate metabolites. After recovery of COCs and in vitro fertilization, a significantly higher number of oocytes developed into blastocysts. The developmental competence of embryos that were originated from ovaries preserved at $15^{\circ}C$ was increased compared to those of 25 or $5^{\circ}C$. The maturation rate of oocytes was not differed between 24 and 36 h at $15^{\circ}C$ but showed lower than control group (71% versus 78%). In vitro-fertilized oocytes from ovaries stored at $25^{\circ}C$ for 24 h or at $5^{\circ}C$ for 24 h had a significantly decreased developmental potentials, but at $15^{\circ}C$ did not (27% versus 29% of blastocysts to develop into day 8). With these results, bovine ovaries can be preserved at $15^{\circ}C$ for 36 h without decreasing developmental capacity of in vitro-fertilized oocyte at least to the blastocyst stage. This information provides valuable information of preserving ovaries for embryo transfer or in vitro embryo production.

키워드

참고문헌

  1. Abe S and Shioya Y. 1996. Effects of temperature and duration of preservation of bovine ovaries in phyiological saline on the development of bovine embryos derived from follicular oocytes matured and fertilized in vitro. Anim. Sci. Technol. Jpn. 67:633-638.
  2. Ball GD, Leibfried ML, Lenz RW, Ax RL, Bavister BD and First NL. 1983. Factors affecting successful in vitro fertilization of bovine follicular oocytes. Biol. Reprod. 28:717-725. https://doi.org/10.1095/biolreprod28.3.717
  3. Bokros CL, Hugdahl JD, Blumenthal SSD and Morejohn. 1996. Proteolytic analysis of polymerized maize tubulin: regulation of microtubule stability to low temperature and $Ca^{2+}$ by the carboxyl terminus of ${\beta}$-tubulin. Plant. Cell. Environ. 19:539-548. https://doi.org/10.1111/j.1365-3040.1996.tb00387.x
  4. Brackett BG and Oliphant G. 1975. Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. Dev. 40:5-11.
  5. Collins GM, Hartley LC and Clunie GJ. 1972. Kidney preservation for transportation. Experimental analysis of optimal perfusate composition. Brit. J. Surg. 59:187-189. https://doi.org/10.1002/bjs.1800590309
  6. Elimadi A and Haddad PS. 2001. Cold preservation-warm reoxygenation increases hepatocyte steady-state $Ca^{{+}{+}}$ and response to Ca++-mobilizing agonist. Am. J. Physiol. Gastrointest. Liver. Physiol. 281:G809-G815. https://doi.org/10.1152/ajpgi.2001.281.3.G809
  7. Guibert EE, Petrenko AY, Balaban CL, Somov AY, Rodriguez JV and Fuller BJ. 2011. Organ Preservation: Current Concepts and New Strategies for the Next Decade. Transfus. Med. Hemother. 38:125-142. https://doi.org/10.1159/000327033
  8. Ideta A, Aoyagi Y, Tsuchiya K, Kamijima T, Nishimiya Y and Tsuda S. 2013. A simple medium enables bovine embryos to be held for seven days at $4^{\circ}C$. Sci. Rep. 1173:1-5.
  9. Ideta Aoyagi Y, Tsuciya K, Nakamura Y, Hayama K, Shirasawa A, Sakaguch K, Tominaga N, Nishimiya Y and Tsuda S. 2015. Prolonging hypothermic storage (4 C) of bovine embryos with fish antifreeze protein. J. Reprod. Dev. 61:1-6. https://doi.org/10.1262/jrd.2014-073
  10. Iwata H, Ohota M, Hashimoto S. and Nagai Y. 2003. Free oxygen radicals are generated at the time of aspiration of oocytes from ovaries that have been stored for a long time. Zygote 11:1-5. https://doi.org/10.1017/S0967199403001011
  11. Jablonski P, Howden B, Marshall V, Scott D. 1980 Evaluation of citrate flushing solution using the isolated perfused rat kidney. Transplantation 30:239-243. https://doi.org/10.1097/00007890-198010000-00001
  12. Lodish H, BerkA and Zipursky SL. 2000. Molecular Cell Biology 4th edition, Freema WH and Company, Section 19. 2
  13. Martino A, Pollard JW and Leibo SP. 1996. Effect of chilling bovine oocytes on their developmental competence. Mol. Reprod. Dev. 45:503-512. https://doi.org/10.1002/(SICI)1098-2795(199612)45:4<503::AID-MRD13>3.0.CO;2-X
  14. Matello CM, Gameiro PA, Bell EL, Mattaini KR, Yang J, Hiller K, Jewell CM, Johnson JR, Irvine DJ, Guarente L, Kelleher JK, Vander Heiden MG, Iliopoulos O. and Stephanogoulos G. 2012. Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia. Nature 481:380-384. https://doi.org/10.1038/nature10602
  15. Matsushita S, Tani T, Kato Y and Tsunoda Y. 2004. Effect of low-temperature bovine ovary storage on the maturation rate and developmental potential of follicular oocytes after in vitro fertilization, parthenogenetic activation, or somatic cell nucleus transfer. Anim. Reprod. Sci. 84:293-301. https://doi.org/10.1016/j.anireprosci.2004.02.008
  16. Rauen U and de Groot H. 2004. New insights into the cellular and molecular mechanisms of cold storage injury. J. Investig. Med. 52:299-309. https://doi.org/10.1136/jim-52-05-29
  17. Reis A, Staines ME, Watt RG, Dolman DF and McEvoy TG. 2002. Embryo production using defined oocyte maturation and zygote culture media following repeated ovum pick-up (OPU) from FSH-stimulated Simmental heifers. Anim. Reprod. Sci. 72:137-151. https://doi.org/10.1016/S0378-4320(02)00075-1
  18. Rosenkraus JCF and First NL. 1991. Culture of bovine zygotes to the blastocyst stage: effect of amino acids and vitamins. Theriogenology 35:266. https://doi.org/10.1016/0093-691X(91)90242-6
  19. Ozturk SS and Palsson BO. 1990. Chemical decomposition of glutamine in cell culture media: effect of media type, pH, and serum concentration. Biotechnol. Prog. 6:121-128. https://doi.org/10.1021/bp00002a005
  20. Senatore EM, Xu J, Suarez Novac MV, Gong G, Lin T, Bela A, Moreno JF, Mannino ME, Tian X, Presicce GA, Wu SC and Du F. 2010. Improved in vitro development of OPU-derived bovine (Bos taurus) embryos by group culture with agarose-embedded helper embryos. Theriogenology 74:1643-1651. https://doi.org/10.1016/j.theriogenology.2010.06.037
  21. Wallin M and Stromberg E. 1995. Cold-stable and cold-adapted microtubules. Int. Rev. Cytol. 157:1-31.
  22. Yang NS, Lu KH and Gordon I. 1990. In vitro fertilization (IVF) and culture (IVC) of bovine oocytes from stored ovaries. Theriogenology 33, 352 (Abstract). https://doi.org/10.1016/0093-691X(90)90776-P