Biomolecules & Therapeutics

  • 제24권6호
  • /
  • Pages.638-649
  • /
  • 2016
  • /
  • 1976-9148(pISSN)
  • /
  • 2005-4483(eISSN)
한국응용약물학회 (The Korean Society of Applied Pharmacology)
권호 표지
DOI QR코드

DOI QR Code

DW2007 Ameliorates Colitis and Rheumatoid Arthritis in Mice by Correcting Th17/Treg Imbalance and Inhibiting NF-κB Activation

  • Lim, Su-Min (Department of Life and Nanopharmaceutical Sciences and Department of Pharmacy, Kyung Hee University) ;
  • Lee, Sang-Yun (Department of Life and Nanopharmaceutical Sciences and Department of Pharmacy, Kyung Hee University) ;
  • Jeong, Jin-Ju (Department of Life and Nanopharmaceutical Sciences and Department of Pharmacy, Kyung Hee University) ;
  • Choi, Hyun Sik (DongWha Pharm Research Institute) ;
  • Chang, Hwan Bong (DongWha Pharm Research Institute) ;
  • Kim, Dong-Hyun (Department of Life and Nanopharmaceutical Sciences and Department of Pharmacy, Kyung Hee University)
  • 투고 : 2016.01.27
  • 심사 : 2016.03.24
  • 발행 : 2016.11.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In the previous study, the rhizome mixture of Anemarrhena asphodeloides and Coptis chinensis (DW2007), improved TNBS-, oxazolone-, or DSS-induced colitis in mice by regulating macrophage activation. Therefore, to understand the effect of DW2007 on the T cell differentiation involved in the adaptive immunity, we measured its effect on both Th17 and Treg cell differentiation in splenocytes, in the lamina propria of mice with DSS-induced colitis (DIC), and in the spleens of mice with collagen-induced arthritis (CIA). Results showed that DW2007 potently inhibited the differentiation of splenocytes into Th17 cells, but increased Treg cell differentiation in vitro. In the colon of wild type and $TLR4^{-/-}$ mice with DIC, DW2007 potently suppressed DSS-induced colon shortening and myeloperoxidase activity. DW2007 also suppressed collagen-induced paw thickening, clinical index, and myeloperoxidase activity in CIA mice. Overall, DW2007 potently suppressed Th17 cell differentiation in mice with CIA and DIC, but increased Treg cell differentiation. Moreover, DW2007 strongly inhibited the expression of TNF-${\alpha}$ and IL-$1{\beta}$, as well as the activation of NF-${\kappa}B$. Based on these findings, DW2007 may ameliorate inflammatory diseases by regulating the innate immunity via the inhibition of macrophage activation and the adaptive immunity via the correction of disturbed Th17/Treg cells.

키워드

참고문헌

  1. Arango Duque, G. and Descoteaux, A. (2014) Macrophage cytokines: involvement in immunity and infectious diseases. Front. Immunol. 5, 491.
  2. Arii, K., Kumon, Y., Sugahara, K., Nakatani, K., Ikeda, Y., Suehiro, T., and Hashimoto, K. (2008) Edaravone inhibits collagen-induced arthritis possibly through suppression of nuclear factor-kappa B. Mol. Immunol. 45, 463-469. https://doi.org/10.1016/j.molimm.2007.05.020
  3. Artis, D. and Spits, H. (2015) The biology of innate lymphoid cells. Nature 517, 293-301. https://doi.org/10.1038/nature14189
  4. Burska, A., Boissinot, M. and Ponchel, F. (2014) Cytokines as biomarkers in rheumatoid arthritis. Mediators Inflamm. 2014, 545493.
  5. Chaudhry, A., Samstein, R. M., Treuting, P., Liang, Y., Pils, M. C., Heinrich, J. M., Jack, R. S., Wunderlich, F. T., Bruning, J. C., Muller, W. and Rudensky, A. Y. (2011) Interleukin-10 signaling in regulatory T cells is required for suppression of Th17 cell-mediated inflammation. Immunity 34, 566-578. https://doi.org/10.1016/j.immuni.2011.03.018
  6. Ebert, E. C. and Hagspiel, K. D. (2011) Gastrointestinal and hepatic manifestations of rheumatoid arthritis. Dig. Dis. Sci. 56, 295-302. https://doi.org/10.1007/s10620-010-1508-7
  7. Granet, C. and Miossec, P. (2004) Combination of the pro-inflammatory cytokines IL-1, $TNF-{\alpha}$ and IL-17 leads to enhanced expression and additional recruitment of AP-1 family members, Egr-1 and $NF-{\kappa}B$ in osteoblast-like cells. Cytokine 26, 169-177. https://doi.org/10.1016/j.cyto.2004.03.002
  8. Isailovic, N., Daigo, K., Mantovani, A. and Selmi, C. (2015) Interleukin-17 and innate immunity in infections and chronic inflammation. J. Autoimmun. 60, 1-11. https://doi.org/10.1016/j.jaut.2015.04.006
  9. Jang, S. E., Jeong, J. J., Hyam, S. R., Han, M. J. and Kim, D. H. (2013) Anticolitic Effect of the rhizome mixture of Anemarrhena asphodeloides and Coptidis chinensis (AC-mix) in mice. Biomol. Ther. (Seoul) 21, 398-404. https://doi.org/10.4062/biomolther.2013.048
  10. Jeong, J. J., Jang, S. E., Hyam, S. R., Han, M. J. and Kim, D. H. (2014) The rhizome mixture of Anemarrhena asphodeloides and Coptidis chinensis ameliorates acute and chronic colitis in mice by inhibiting the binding of lipopolysaccharide to TLR4 and IRAK1 phosphorylation. Evid. Based Complement. Alternat. Med. 2014, 809083.
  11. Kalim, K. W., Basler, M., Kirk, C. J. and Groettrup, M. (2012) Immunoproteasome subunit LMP7 deficiency and inhibition suppresses Th1 and Th17 but enhances regulatory T cell differentiation. J. Immunol. 189, 4182-4193. https://doi.org/10.4049/jimmunol.1201183
  12. Kang, G. D., Lim, S. and Kim, D. H. (2015) Oleanolic acid ameliorates dextran sodium sulfate-induced colitis in mice by restoring the balance of Th17/Treg cells and inhibiting $NF-{\kappa}B$ signaling pathway. Int. Immunopharmacol. 29, 393-400. https://doi.org/10.1016/j.intimp.2015.10.024
  13. Lee, S. Y., Jeong, J. J., Le, T. H., Eun, S. H., Nguyen, M. D., Park, J. H. and Kim, D. H. (2015a) Ocotillol, a majonoside R2 metabolite, ameliorates 2,4,6-trinitrobenzenesulfonic acid-induced colitis in mice by restoring the balance of Th17/Treg cells. J. Agric. Food Chem. 63, 7024-7031. https://doi.org/10.1021/acs.jafc.5b02183
  14. Lee, S. Y., Jeong, J. J., Kim, K. A. and Kim, D. H. (2015b) Lactobacillus sakei OK67 ameliorates collagen-induced arthritis in mice by inhibiting $NF-{\kappa}B$ activation and restoring Th17/Treg cell balance. J. Funct. Foods 18, Part A 501-511. https://doi.org/10.1016/j.jff.2015.08.006
  15. Li, Q. and Verma, I. M. (2002) $NF-{\kappa}B$ regulation in the immune system. Nat. Rev. Immunol. 2, 725-734. https://doi.org/10.1038/nri910
  16. Liu, B., Tonkonogy, S. L. and Sartor, R. B. (2011) Antigen-presenting cell production of IL-10 inhibits T-helper 1 and 17 cell responses and suppresses colitis in mice. Gastroenterology 141, 653-662. https://doi.org/10.1053/j.gastro.2011.04.053
  17. Liu, W., Guo, W., Guo, L., Gu, Y., Cai, P., Xie, N., Yang, X., Shu, Y., Wu, X., Sun, Y. and Xu, Q. (2014) Andrographolide sulfonate ameliorates experimental colitis in mice by inhibiting Th1/Th17 response. Int. Immunopharmacol. 20, 337-345. https://doi.org/10.1016/j.intimp.2014.03.015
  18. Lopez, A. and Peyrin-Biroulet, L. (2013) 5-Aminosalicylic acid and chemoprevention: does it work? Dig. Dis. 31, 248-253. https://doi.org/10.1159/000353806
  19. Mellado, M., Martinez-Munoz, L., Cascio, G., Lucas, P., Pablos, J. L. and Rodriguez-Frade, J. M. (2015) T cell migration in rheumatoid arthritis. Front. Immunol. 6, 384.
  20. Monteleone, I., Sarra, M., Pallone, F. and Monteleone, G. (2012) Th17-related cytokines in inflammatory bowel diseases: friends or foes? Curr. Mol. Med. 12, 592-597. https://doi.org/10.2174/156652412800620066
  21. Noack, M. and Miossec, P. (2014) Th17 and regulatory T cell balance in autoimmune and inflammatory diseases. Autoimmun. Rev. 13, 668-677. https://doi.org/10.1016/j.autrev.2013.12.004
  22. Parker, L. C., Prince, L. R. and Sabroe, I. (2007) Translational mini-review series on Toll-like receptors: networks regulated by Toll-like receptors mediate innate and adaptive immunity. Clin. Exp. Immunol. 147, 199-207. https://doi.org/10.1111/j.1365-2249.2006.03203.x
  23. Perkins, N. D. and Gilmore, T. D. (2006) Good cop, bad cop: the different faces of $NF-{\kappa}B$. Cell Death Differ. 13, 759-771. https://doi.org/10.1038/sj.cdd.4401838
  24. Qu, N., Xu, M., Mizoguchi, I., Furusawa, J., Kaneko, K., Watanabe, K., Mizuguchi, J., Itoh, M., Kawakami, Y. and Yoshimoto, T. (2013) Pivotal roles of T-helper 17-related cytokines, IL-17, IL-22, and IL-23, in inflammatory diseases. Clin. Dev. Immunol. 2013, 968549.
  25. Robinson, M. (1998) Medical therapy of inflammatory bowel disease for the 21st century. Eur. J. Surg. Suppl. 582, 90-98.
  26. Sarra, M., Pallone, F., Macdonald, T. T. and Monteleone, G. (2010) IL-23/IL-17 axis in IBD. Inflamm. Bowel Dis. 16, 1808-1813. https://doi.org/10.1002/ibd.21248
  27. Shen, F., Ruddy, M. J., Plamondon, P. and Gaffen, S. L. (2005) Cytokines link osteoblasts and inflammation: microarray analysis of interleukin-17- and TNF-alpha-induced genes in bone cells. J. Leukoc. Biol. 77, 388-399. https://doi.org/10.1189/jlb.0904490
  28. Szekanecz, Z. and Koch, A. E. (2007) Macrophages and their products in rheumatoid arthritis. Curr. Opin. Rheumatol. 19, 289-295. https://doi.org/10.1097/BOR.0b013e32805e87ae
  29. Tao, F., Qian, C., Guo, W., Luo, Q., Xu, Q., Sun, Y. (2013) Inhibition of Th1/Th17 responses via suppression of STAT1 and STAT3 activation contributes to the amelioration of murine experimental colitis by a natural flavonoid glucoside icariin. Biochem. Pharmacol. 85, 798-807. https://doi.org/10.1016/j.bcp.2012.12.002
  30. Wallace, K. L., Zheng, L. B., Kanazawa, Y. and Shih, D. Q. (2014) Immunopathology of inflammatory bowel disease. World J. Gastroenterol. 20, 6-21. https://doi.org/10.3748/wjg.v20.i1.6
  31. Yan, J. W., Wang, Y. J., Peng, W. J., Tao, J. H., Wan, Y. N., Li, B. Z., Mei, B., Chen, B., Yao, H., Yang, G. J., Li, X. P., Ye, D. Q. and Wang, J. (2014) Therapeutic potential of interleukin-17 in inflammation and autoimmune diseases. Expert Opin. Ther. Targets 18, 29-41. https://doi.org/10.1517/14728222.2013.843669
  32. Yang, J., Sundrud, M. S., Skepner, J. and Yamagata, T. (2014) Targeting Th17 cells in autoimmune diseases. Trends Pharmacol. Sci. 35, 493-500. https://doi.org/10.1016/j.tips.2014.07.006

피인용 문헌

  1. The Mixture of Anemarrhena asphodeloides and Coptis chinensis Attenuates High-Fat Diet-Induced Colitis in Mice vol.45, pp.05, 2017, https://doi.org/10.1142/S0192415X17500550
  2. Huangqin-Tang and Ingredients in Modulating the Pathogenesis of Ulcerative Colitis vol.2017, 2017, https://doi.org/10.1155/2017/7016468
  3. Knockout Mouse vol.26, pp.3, 2018, https://doi.org/10.4062/biomolther.2017.103
  4. Two polysaccharides from Porphyra modulate immune homeostasis by NF-κB-dependent immunocyte differentiation vol.10, pp.4, 2016, https://doi.org/10.1039/c9fo00023b
  5. Immunomodulatory activity of R-phycoerythrin from Porphyra haitanensis via TLR4/NF-κB-dependent immunocyte differentiation vol.11, pp.3, 2020, https://doi.org/10.1039/c9fo02444a