Maxillofacial Plastic and Reconstructive Surgery

  • Volume 38
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  • Pages.17.1-17.6
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  • 2016
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  • 2288-8101(pISSN)
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  • 2288-8586(eISSN)
Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
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Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration

  • Yoo, Chae-Kyung (Department of Translational Medicine, Graduate School of Biomedical Science and Engineering, Hanyang University) ;
  • Jeon, Jae-Yun (Department of Dentistry/Division of Oral and Maxillofacial Surgery, College of Medicine, Hanyang University) ;
  • Kim, You-Jin (Korean Minjok Leadership Academy) ;
  • Kim, Seong-Gon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Hwang, Kyung-Gyun (Department of Translational Medicine, Graduate School of Biomedical Science and Engineering, Hanyang University)
  • Received : 2016.01.21
  • Accepted : 2016.03.10
  • Published : 2016.12.31
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Abstract

Background: The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR). Methods: Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4',6-diamidino-2-phenylindole (DAPI) fluorescence staining. Results: Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (P < 0.05); however, at respective time points, cell viability was not significantly different between the two groups (P > 0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days (P < 0.05). Conclusions: These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.

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