Abstract
Supervised residue trials for mandipropamid in Korean cabbage(Brassica campestris L.) were conducted to establish its pre-harvest residue limit (PHRL), a criterion to ensure the safety of the terminal pesticide residue during cabbage production. Tissues of Korean cabbage were collected at 0, 1, 3, 5, 7, and 10 days after mandipropamid application and subjected to residue analysis. The analytical method was validated by recoveries ranging from 88.2-92.2% at two levels (0.4 and $2.0mg{\cdot}kg^{-1}$), and a limit of quantitation (LOQ) of $0.04mg{\cdot}kg^{-1}$. Mandipropamid residues in Korean cabbage gradually decreased over time. The dissipation rate of the residue would be affected by intrinsic degradation of the compound along with dilution resulting from the fast growth of Korean cabbage. The decay pattern was well fitted by simple first-order kinetics. Biological half-lives of mandipropamid in Korean cabbage ranged from 3.9-4.0 days in two field conditions. Calculated by the regression curve of mandipropamid dissipation, the PHRLs of mandipropamid in Korean cabbage were recommended as 11.07-12.19 and $5.76-6.05mg{\cdot}kg^{-1}$ for 10 and 5 days prior to harvest, respectively.
본 연구는 엇갈이배추 재배기간 중 mandelamide계 살균제인 mandipropamid를 수확 10일전 1회 살포한 후 각각 0(2시간 이내), 1, 3, 5, 7, 및 10일 후에 엇갈이배추 시료를 채취하여 mandipropamid 잔류량을 분석, 통계학적 생물학적 반감기를 산출하여 생산단계 농약잔류허용기준(Pre-Harvest Residue Limit; PHRL)을 설정하고자 하였다. Mandipropamid의 평균 회수율은 0.4 및 $2.0mg{\cdot}kg^{-1}$의 두 수준에서 각각 $88.2{\pm}3.2%$와 $92.2{\pm}2.0%$이었으며, 분석정량한계는 $0.04mg{\cdot}kg^{-1}$이었다. Mandipropamid를 기준량으로 1회 살포 시 엇갈이배추 중의 생물학적 반감기는 포장 1 및 2에서 각각 3.9일과 4.0일 이었고, 생체중 증가가 mandipropamid의 잔류량 감소에 상당한 영향을 나타내었다. 잔류감소 회귀식을 이용한m andipropamid의 생산단계 잔류허용기준은 수확 10일전의 경우 $11.07-12.19mg{\cdot}kg^{-1}$ 수준, 수확 5일전의 경우 $5.76-6.05mg{\cdot}kg^{-1}$ 수준인 것이 바람직한 것으로 판단 되었다.
