Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Innate Color Preference of Zebrafish and Its Use in Behavioral Analyses

  • Park, Jong-Su (Department of Biology, Chungnam National University) ;
  • Ryu, Jae-Ho (Department of Biology, Chungnam National University) ;
  • Choi, Tae-Ik (Department of Biology, Chungnam National University) ;
  • Bae, Young-Ki (Comparative Biomedicine Research Branch, National Cancer Center) ;
  • Lee, Suman (Division of Structural and Functional Genomics, Center for Genome Science, National Research Institute of Health) ;
  • Kang, Hae Jin (Department of Biology, Chungnam National University) ;
  • Kim, Cheol-Hee (Department of Biology, Chungnam National University)
  • Received : 2016.07.12
  • Accepted : 2016.10.12
  • Published : 2016.10.31
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Abstract

Although innate color preference of motile organisms may provide clues to behavioral biases, it has remained a longstanding question. In this study, we investigated innate color preference of zebrafish larvae. A cross maze with different color sleeves around each arm was used for the color preference test (R; red, G; green, B; blue, Y; yellow). The findings showed that 5 dpf zebrafish larvae preferred blue over other colors (B > R > G > Y). To study innate color recognition further, tyrosinase mutants were generated using CRISPR/Cas9 system. As a model for oculocutaneous albinism (OCA) and color vision impairment, tyrosinase mutants demonstrated diminished color sensation, indicated mainly by hypopigmentation of the retinal pigment epithelium (RPE). Due to its relative simplicity and ease, color preference screening using zebrafish larvae is suitable for high-throughput screening applications. This system may potentially be applied to the analysis of drug effects on larval behavior or the detection of sensory deficits in neurological disorder models, such as autism-related disorders, using mutant larvae generated by the CRISPR/Cas9 technique.

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References

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