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Isoflavone, β-Glucan Content and Antioxidant Activity of Defatted Soybean Powder by Bioconversion with Lentinula edodes

표고 균사에 의한 탈지 대두박 생물전환 발효물의 이소플라본, 베타글루칸 함량 및 항산화활성

  • Jung, Tae-Dong (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Shin, Gi-Hae (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Kim, Jae-Min (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Choi, Sun-Il (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Jin-Ha (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Sang Jong (STR biotech Co., LTD.) ;
  • Heo, In Young (STR biotech Co., LTD.) ;
  • Park, Seon Ju (STR biotech Co., LTD.) ;
  • Oh, Sea-Kwan (Crop Post-harvest Technology Division, National Institute of Crop Science, RDA) ;
  • Woo, Koan-Sik (Crop Post-harvest Technology Division, National Institute of Crop Science, RDA) ;
  • Lim, Jae Kag (Department of Chemical Engineering and Biotechnology, Korea Polytechnic University) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
  • 정태동 (강원대학교 식품생명공학과) ;
  • 신기해 (강원대학교 식품생명공학과) ;
  • 김재민 (강원대학교 식품생명공학과) ;
  • 최선일 (강원대학교 식품생명공학과) ;
  • 이진하 (강원대학교 식품생명공학과) ;
  • 이상종 ((주)에스티알바이오텍) ;
  • 허인영 ((주)에스티알바이오텍) ;
  • 박선주 ((주)에스티알바이오텍) ;
  • 오세관 (국립식량과학원 수확후이용과) ;
  • 우관식 (국립식량과학원 수확후이용과) ;
  • 임재각 (한국산업기술대학교 생명과학공학과) ;
  • 이옥환 (강원대학교 식품생명공학과)
  • Received : 2016.07.08
  • Accepted : 2016.08.18
  • Published : 2016.10.28

Abstract

This study investigated the isoflavone content, total phenol content, antioxidant activities (DPPH radical scavenging and oxygen radical absorbance capacity) and ${\beta}$-glucan content of defatted soybean extracts by bioconversion. Soybean was fermented with Lentinula edodes using submerged liquid fermentation system. Defatted soybean powder prepared by hexane (HDS; hexane defatted soybean) and ethanol (EDS; ethanol defatted soybean). The major components of non-fermented HDS (NFHDS) and EDS (NFEDS) were glucoside, such as daidzin, glycitin and genistin. During the bioconversion processing, isoflavone glucoside converted into aglycone such as daidzein, glycitein and genistein. The highest total isoflavone contents of fermented HDS (FHDS) were $2577.96{\mu}g/mL$, and the lowest total isoflavone contents of NFEDS were $428.27{\mu}g/mL$. The highest total phenol contents of fermented EDS (FEDS) was 42.34 mg GAE/g. DPPH radical scavenging and ORAC value were 31.30 to 59.92% and 247.48 to $786.36{\mu}M\;TE/g$ in non-fermented defatted soybean and fermented soybean, respectively. ${\beta}$-Glucan contents were 0.09 to 0.11% in non-fermented defatted soybean and fermented soybean, respectively. These results indicate that fermented soybean could be used as natural antioxidants for the development of functional foods.

본 연구는 생물전환된 대두의 isoflavone 함량, 총 페놀함량, 항산화능(DPP radical 소거능, ORAC 지수) 및 ${\beta}$-Glucan 함량을 측정하였다. Isoflavone의 경우 추출용매에 상관없이 배당체가 모두 비배당체로 전환되는 것을 확인하였다. Total isoflavone 함량의 경우 hexane 탈지 대두박 발효물에서 $2577.96{\mu}g/mL$으로 가장 높은 값을 나타냈으며, ethanol 탈지 대두박 비발효물에서 $428.27{\mu}g/mL$으로 가장 낮은 값을 나타내었다. 총 페놀 함량은 대두 원물에서 39.44 mg GAE/g으로 나타났으며, ethanol 탈지 대두박 비발효물 및 hexane 탈지 대두박 비발효물은 27.07, 27.75 mg GAE/g으로 대두 원물보다 다소 낮은 값을 나타냈다. 생물전환된 대두의 총 페놀 함량은 hexane 탈지 대두박 발효물 41.61 mg GAE/g, ethanol 탈지 대두박 발효물 42.34 mg GAE/g으로 비발효물에 비해 약 1.5배 가량 증가된 함량을 보였다. DPPH radical 소거능의 경우 대두 원물에서 51.10%의 소거능을 나타내었고 hexane 탈지 대두박 비발효물에서 50.51%, ethanol 탈지 대두박 비발효물은 43.27%의 소거능을 나타냈다. 생물전환된 ethanol 탈지 대두박 발효물에서 59.92%로 radical 소거능이 증가되었지만 hexane 탈지 대두박 발효물은 31.30%로 비발효물에 비해 낮은 radical 소거활성을 보였다. ORAC 지수는 대두 원물이 $384.47{\mu}M\;TE/g$을 보였으며, hexane 탈지 대두박 비발효물 및 ethanol 탈지 대두박 비발효물은 318.52, $247.48{\mu}M\;TE/g$으로 나타났다. 생물전환된 hexane 탈지 대두박 발효물은 $786.36{\mu}M\;TE/g$, ethanol 탈지 대두박 발효물에서 $721.96{\mu}M\;TE/g$으로 비발효물에 비해 ORAC 지수가 증가하는 것으로 나타났다. ${\beta}$-Glucan 함량은 0.09~0.11%의 범위로 나타났으며 대두 원물과 ethanol 탈지 대두박 발효물에서 가장 높은 0.11%를 보였고 hexane 탈지 대두박 비발효물과 hexane 탈지 대두박 발효물에서 0.09%로 가장 낮은 ${\beta}$-glucan 함량을 보였지만, 추출용매 및 생물전환에 따른 ${\beta}$-glucan 함량의 큰 차이는 나타나지 않았다.

Keywords

References

  1. Kim, J.A., Jung, W.S., Chun, S.C., Yu, C.Y., Ma, K.H., Gwag, J.G. and Chung, I.M.: A correlation between the level of phenolic compounds and the antioxidant capacity in cooked-with-rice and vegetable soybean (Glycine max L.) varieties. Eur Food Res Technol., 224, 259-270 (2006). https://doi.org/10.1007/s00217-006-0377-y
  2. Malencic, D., Popovic, M. and Miladinovic, J.: Phenolic content and antioxidant properties of soybean (Glycine max (L.) Merr.) seeds. Molecules, 12, 576-581 (2007). https://doi.org/10.3390/12030576
  3. Jeon, S.H., Lee, K. and Byoun, K.E.: Studies on changes of isoflavone and nutrients during germination of soybean varieties. Korean J Human Ecology, 14, 485-489 (2005).
  4. Lim, A.K., Jung, M.J., Kim, D.W., Hong, J.H., Jung, H.K., Kim, K.S. and Kim, D.I.: An Extrapolation Concentration Decision Effect Antihyperlipidemic of Aglycone Isoflavone from Biotransformation Soybean on the Fed High-Fat Diet Rats. J Korean Soc Food Sci Nutr., 38, 1167-1173 (2009). https://doi.org/10.3746/jkfn.2009.38.9.1167
  5. Kim, I.B., Shin, S., Lim, B.L., Seong, G.S. and Lee, Y.E.: Bioconversion of soybean isoflavone by Lactobacillus plantarum and Bifidobacterium longum. Korean J Food Cookery Sci., 26, 214-219 (2010).
  6. Lee, K.J., Gu, M.J., Roh, J.H., Jung, P,M. and Ma, J.Y.: Quanctitative Analysis of Bioconversion Constituents of Insampeadock-san Using Various Fermented Bacteria. Yakhak Hoeji., 57, 167-172 (2013).
  7. Kim, M.H.: Biological Activity of Ethanol extracts from Fermented Opuntia humifusa with 3 Different Mushroom Mycelia. J Korean Soc Food Sci Nutr., 28, 620-627 (2015). https://doi.org/10.9799/ksfan.2015.28.4.620
  8. Jung, T.D., Shin, G.H., Kim, J.M., Choi, S.I., Lee, S.J., Heo, I.Y,, Park, S.J., Kim, H.T., Kang, B.K. and Lee, O.K.: Assessment of Validation Method for Bioactive Contents of Fermented Soybean Extracts by Bioconversion and Their Antioxidant Activities. J Korean Soc Food Sci Nutr., 45, 680-689 (2016). https://doi.org/10.3746/jkfn.2016.45.5.680
  9. Halliwell, B., Aeschbach, R., Lliger, J. and Aruoma, O.I.: The characterization of antioxidants. Food Chem Toxicol., 33, 601-617 (1995). https://doi.org/10.1016/0278-6915(95)00024-V
  10. Larson, R.A.: The antioxidants of higher plants. Phytochemistry, 27, 969-978 (1988). https://doi.org/10.1016/0031-9422(88)80254-1
  11. Saito, M., Sakagami, H. and Fujisawa, S.: Cytotoxicity and apoptosis induction by butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). Anticancer Res., 23, 4693-4701 (2002).
  12. KFDA. Health Functional Food Code. Korea Food & Drug Administration, Seoul, Korea. pp. 340-343 (2013).
  13. Ismail, A., Marjan, Z.M. and Foong, C.W.: Total antioxidant activity and phenolic content in selected vegetables. Food Chem., 87, 581-586 (2004). https://doi.org/10.1016/j.foodchem.2004.01.010
  14. Mensor, L.L., Menezes, F.S., Leito, G.G., Reis, A.S., Santos, T.C.D., Coube, C.S. and Leitao, S.G.: Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Res., 15, 127-130 (2001). https://doi.org/10.1002/ptr.687
  15. Wang, H., Cao, G. and Prior, R.L.: Total antioxidant capacity of fruits. J Agric Food Chem., 44, 701-705 (1996). https://doi.org/10.1021/jf950579y
  16. McCleary, B.V. and Glennie-Holmes, M.: Enzymic quantification of (1-3)(1-4)-$\beta$-D-glucan in barley and malt. J Inst Brew., 91, 285-295 (1985). https://doi.org/10.1002/j.2050-0416.1985.tb04345.x
  17. Wang, Y.N., Wu, W., Chen, HC. and Fang, H.: Genistein protects against UVB-induced senescence-like characteristics in human dermal fibroblast by p66Shc down-regulation. J dermatological Sci., 58, 19-27 (2010). https://doi.org/10.1016/j.jdermsci.2010.02.002
  18. Jeong, E.J., Kim, J.Y., Moon, S.H. and Park, K.Y.: Characteristics, Antioxidative Activities and Growth Inhibitory Effects in AGS Human Gastric Adenocarcinoma Cells of Soymilk Fermented by Bacillus subtilis KC-3 during Fermentation. J Korean Soc Food Sci Nutr., 39, 1113-1118 (2010). https://doi.org/10.3746/jkfn.2010.39.8.1113
  19. Shin, D.B., Lee, D.W., Yang, R. and Kim, J.A.: Antioxidative properties and flavonoids contents of matured Citrus peel extracts. Food Sci Biotechnol., 15, 357-362 (2006).
  20. Lee, G.W., Park, S.M., Yoo, Y.C. and Cho, Y.H.: Effect of ponciri fructus extracts fermented with ganoderma lucidum on the collagen synthesis and expression of matrix metalloproteinase-1. KSBB Journal, 28, 106-114 (2013). https://doi.org/10.7841/ksbbj.2013.28.2.106
  21. Foti, M.C., Daquino, C. and Geraci, C.: Electron-transfer reaction of cinnamic acids and their methyl esters with the DPPH radical in alcoholic solutions. J Org Chem., 69, 2309-2314 (2004). https://doi.org/10.1021/jo035758q
  22. Kim, E.J., Moon, J.S. and Choe, T.B.: Inhibition of tyrosinase by bio-conversion Morus alba extract. Kor J Aesthet Cosmetol., 11, 845-854 (2013).
  23. Lee, J.S., Lee, S.H., Jang, Y.M., Lee, J.D., Lee, B.H. and Jung, J.Y.: Macrophage and anticancer activities of feed additives on $\beta$-glucan from Schizophyllum commune in breast cancer cells. J Korean Soc Food Sci Nutr., 40, 949-955 (2011). https://doi.org/10.3746/jkfn.2011.40.7.949
  24. Newman, R.K., Lewis, S.E., Newman, C.W., Boik, R.J. and Ramage, R.T.: Hypocholesterolemic effect of barley foods on healthy men. Nutr Rep Int., 39, 749-760 (1989).
  25. Park, H.J., Kim, Y.B., Kang, T.S., Jung, I.S., Kim, K.Y. and Jeong, H.S.: Immunomodulatory activities of oat bran extracts with different extraction conditions. Korean J Food Sci Technol., 37, 103-107 (2005).
  26. Xu, X.M., Jun, J.Y. and Jeong, I.H.: A study on the antioxidant activity of Hae-Songi mushroom (Hypsizigus marmoreus) hot water extracts. J Korean Soc Food Sci Nutr., 36, 1351-1357 (2007). https://doi.org/10.3746/jkfn.2007.36.11.1351
  27. Lee, H.D. and Lee, G.S.: $\beta$-glucan and glucosamine contents in various cereals cultured with mushroom mycelia. Korean J Mycol., 37, 167-172 (2009). https://doi.org/10.4489/KJM.2009.37.2.167
  28. Dudonne, S., Vitrac, X., Coutiere, P., Woillez, M. and Merillon, J.M.: Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J Agric Food Chem., 57, 1768-1774 (2009). https://doi.org/10.1021/jf803011r
  29. Kim, M.J., Chu, W.M. and Park, E.J.: Antioxidant and antigenotoxic effects of shiitake mushrooms affected by different drying methods. J Korean Soc Food Sci Nutr., 41, 1041-1048 (2012). https://doi.org/10.3746/jkfn.2012.41.8.1041