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Evaluation of in vivo Genotoxicity of Plant Flavonoids, Quercetin and Isoquercetin

식물유래 플라보노이드 Quercetin과 Isoquercetin의 생체 내 유전독성평가

  • Pak, Bumsoo (Department of Biotechnology, Hoseo University) ;
  • Han, Sehee (Department of Biotechnology, Hoseo University) ;
  • Lee, Jiyeon (Department of Biotechnology, Hoseo University) ;
  • Chung, Young-Shin (Department of Biotechnology, Hoseo University)
  • Received : 2016.09.20
  • Accepted : 2016.09.30
  • Published : 2016.10.28

Abstract

In vivo genotoxic potential of isoquercetin, a plant common flavonoid, in comparison with quercetin was investigated for the DNA breakage and the clastogenicity endpoints. Male ICR mice were administered by oral gavage for 3 days with $3{\times}0.5%$ carboxymethyl cellulose (CMC), 3 ${\times}$ isoquercetin (250, 500 mg/kg/day), 3 ${\times}$ quercetin (250, 500 mg/kg/day) and 2 ${\times}$ ethyl methanesulfonate (EMS, 200 mg/kg/day). Tissues were collected 48 hours after the first treatment and within 3 hours after the last treatment. The DNA damages were evaluated using Comet assay in liver and stomach, while the clastogenicities were determined using micronucleus test in bone marrow of same animals. The treatment of isoquercetin as well as quercetin did not cause the DNA damages in liver and stomach, and not induce the frequencies of micronucleus polychromatic erythrocytes in bone marrow. In conclusion, isoquercetin as well as quercetin did not cause the DNA breakages and the chromosomal damages in vivo system in these study conditions.

Acknowledgement

Supported by : 호서대학교

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