Tuberculosis and Respiratory Diseases

The Korean Academy of Tuberculosis and Respiratory Diseases (대한결핵및호흡기학회)
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Performance of the BacT Alert 3D System Versus Solid Media for Recovery and Drug Susceptibility Testing of Mycobacterium tuberculosis in a Tertiary Hospital in Korea

  • Kim, Seoung-Cheol (Department of Microbiology, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine) ;
  • Jeon, Bo-Young (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Kim, Jin-Sook (Food & Drug Administration) ;
  • Choi, In Hwan (National Masan Tuberculosis Hospital) ;
  • Kim, Jiro (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Woo, Jeongim (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Kim, Soojin (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Lee, Hyeong Woo (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Sezim, Monoldorova (Department of Biomedical Laboratory Science, College of Health Science, Yonsei University) ;
  • Cho, Sang-Nae (Department of Microbiology, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine)
  • Received : 2016.02.26
  • Accepted : 2016.08.19
  • Published : 2016.10.05
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Abstract

Background: Tuberculosis (TB) is a major health problem, and accurate and rapid diagnosis of multidrug-resistant (MDR) and extended drug-resistant (XDR) TB is important for appropriate treatment. In this study, performances of solid and liquid culture methods were compared with respect to MDR- and XDR-TB isolate recovery and drug susceptibility testing. Methods: Sputum specimens from 304 patients were stained with Ziehl-Neelsen method. Mycobacterium tuberculosis (Mtb) isolates were tested for recovery on $L{\ddot{o}wenstein$-Jensen (LJ) medium and the BacT Alert 3D system. For drug susceptibility testing of Mtb, isolates were evaluated on M-KIT plates and the BacT Alert 3D system. Results: The recovery rates were 94.9% (206/217) and 98.2% (213/217) for LJ medium and the BacT Alert 3D system, respectively (kappa coefficient, 0.884). The rate of drug resistance was 13.4% for at least one or more drugs, 6.0% for MDR-TB and 2.3% for XDR-TB. M-KIT plate and BacT 3D Alert 3D system were comparable in drug susceptibility testing for isoniazid (97.7%; kappa coefficient, 0.905) and rifampin (98.6%; kappa coefficient, 0.907). Antibiotic resistance was observed using M-KIT plates for 24 of the total 29 Mtb isolates (82.8%). Conclusion: The liquid culture system showed greater reduction in the culture period, as compared with LJ medium; however, drug susceptibility testing using M-KIT plates was advantageous for simultaneous testing against multiple drug targets.

Keywords

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