Amino Acid, Amino Acid Metabolite, and GABA Content of Three Domestic Tomato Varieties

  • Ahn, Jun-Bae (Dept. of Food Service & Culinary Arts, Seowon University)
  • Received : 2016.09.02
  • Accepted : 2016.09.26
  • Published : 2016.09.30

Abstract

To determine the nutritional value of domestic tomatoes, the levels of amino acids, amino acid metabolites, and the bioactive compound ${\gamma}-aminobutyric-acid$ (GABA) were analyzed in three domestic tomato varieties (Rafito, Momotaro, and Medison). Eighteen free amino acids were found, and total free amino acid content was 3,810.21~4,594.56 mg/100 g (dry weight). L-glutamic acid (L-Glu) was the most abundant amino acid, ranging from 1,866.60 mg/100 g for Momotaro to 2,417.45 mg/100 g for Medison. The next most abundant amino acids were L-glutamine (L-Gln) and L-aspartic acid (L-Asp). The three tomato varieties had a good balance of all the essential amino acids except tryptophan. Total essential amino acid content was 274.26~472.71 mg/100 g (dry weight). The following amino acid metabolites were found: L-carnitine (L-Car), hydroxylysine (Hyl), o-phosphoethanolamine (o-Pea), phosphoserine (p-Ser), ${\beta}-alanine$ (${\beta}-Ala$), N-methyl-histidine (Me-His), ethanolamine (EtNH2),and L-citrulline(L-Cit). Large quantities of GABA were found in all three varieties: 666.95-868.48 mg/100g (dry weight). These results support the use of these tomato varieties as nutritious food materials.

Keywords

References

  1. Friedman M (2002). Tomato glycoalkaloids: role in the plant and in the diet. J Agric Food Chem 50(21):5751-5780. https://doi.org/10.1021/jf020560c
  2. Frusciante L, Carli P, Ercolano MR, Pernice R, Di Matteo A, Fogliano V, Pellegrini N (2007). Antioxidant nutritional quality of tomato. Mol Nutr Food Res 51(5):609-617. https://doi.org/10.1002/mnfr.200600158
  3. Lee HB, Yang CB, Yu TJ (1972). Studies on the chemical composition of some fruit vegetables and fruits in Korea(I). Korean J Food Sci Technol 4(1):36-43.
  4. Edward G (1999). Tomatoes, tomato-based products, lycopene and cancer. J Natl Cancer I 91(4): 317-331. https://doi.org/10.1093/jnci/91.4.317
  5. Edward G, Eric BR, Yan L, Meir JS, Walter CW (2002). A prospective study of tomato products, lycopene and prostate cancer risk. J Natl Cancer I 94(5):391-398. https://doi.org/10.1093/jnci/94.5.391
  6. Oshima S, Ojima F, Sakamoto H, Ishiguro Y, Terao J (1998). Supplementation with carotenoids inhibits singlet oxigen-mediated oxidation of human plasma low-density lipoprotein. J Agric Food Chem 44(8):2306-2309. https://doi.org/10.1021/jf950350i
  7. Stahl W, Heinrich U, Wiseman S, Eichler O, Sies H, Tronnier H (2001). Dietary tomato paste protects against ultraviolet light-induced erythema in human. J Nutr 131(5):1449-1451. https://doi.org/10.1093/jn/131.5.1449
  8. Choi SH, Kim DH, Kim DS (2011) Comparison of ascorbic acid, lycopene, ${\beta}$-carotene and ${\alpha}$-carotene contents in processed tomato products, tomato cultivar and part. Korean J Culinary Res 17(4):263-272.
  9. Lee MS, Kim GH (1986). Quality evaluation of raw tomato fruits. J Food Sci 18(5):335-338.
  10. Ryu BH, Moon KD, Kim SD, Sohn TH (1990). The changes of hardness and mineral components of tomato fruits during ripening. J Korean Soc Food Nutr 19(2):115-120.
  11. Choi SH, Ahn JB (2014) Functional properties of the lycopene cultivar of cherry tomato. Korean J Culinary Res 20(6):115-127.
  12. Nicolas B, Hilled F (2004). GABA in plants: just a metabolite? Trends Plant Sci 9(3):110-115. https://doi.org/10.1016/j.tplants.2004.01.006
  13. Chang JS, Lee BS, Kim YG (1992). Changes in ${\gamma}$-aminobutyric acid (GABA) and the main constituents by a treatment conditions and of anaerobically treated green tea leaves. Korean J Food Sci Technol 24(4):315-319.
  14. Leventhal AG, Wang YC, Pu ML, Zhou YF, Ma Y (2003). GABA and its agonists improved visual cortical function in senescent monkeys. Science 300(5620):812-815. https://doi.org/10.1126/science.1082874
  15. Friedman M, Levin CE (2008). Review of methods for the reduction of dietary content and toxicity of acrylamide. J Agric Food Chem 56 (15):6113-6140. https://doi.org/10.1021/jf0730486
  16. Choi SH, Lee SH, Kim HJ, Lee IS, Nobuyuki K, Levin CE, Friedman M (2010). Changes in free amino acid, phenolic, chlorophyll, carotenoid, and glycoalkaloid contents in tomatoes during 11 stages of growth and inhibition of cervical and lung human cancer cells by green tomato extracts. J Agric Food Chem 58(13):7547-7556. https://doi.org/10.1021/jf100162j
  17. Bremer J (1983). Carnitine-metabolism and functions. Physiol Rev 63(4):1420-1480. https://doi.org/10.1152/physrev.1983.63.4.1420
  18. Shelp BJ, Bown AW, McLean MD (1999). Metabolism and functions of gamma aminobutyric acid. Trends Plant Sci 4(11):446-452. https://doi.org/10.1016/S1360-1385(99)01486-7
  19. Bowery NG, Smart TG (2006). GABA and glycine as neurotransmitters: a brief history. Br J Pharmacol 147(Suppl.1):S109-S119. https://doi.org/10.1038/sj.bjp.0706439
  20. Abe Y, Umemura S, Sugimoto K, Hirawa N, Kato Y, Yokoyama N, Yokoyama T, Iwai J, Ishii M (1995). Effect of green tea rich in ${\gamma}$-aminobutyric acid on blood pressure of Dahl salt-sensitive rats. Am J Hypertens 8(1):74-79. https://doi.org/10.1016/0895-7061(94)00141-W
  21. Kinnersley AM, Turano FJ (2000) ${\gamma}$-Aminobutyric acid (GABA) and plant responses to stress. Crit Rev Plant Sci 19(6):479-509. https://doi.org/10.1016/S0735-2689(01)80006-X
  22. Deewatthanawonga R, Rowellb P, Watkinsa CB (2010). ${\gamma}$-Aminobutyric acid (GABA) metabolism in $CO_2$ treated tomatoes. Postharvest Biol Technol 57(2):97-105. https://doi.org/10.1016/j.postharvbio.2010.03.007