Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Discrimination of Korean ginseng (Panax ginseng Meyer) cultivar Chunpoong and American ginseng (Panax quinquefolius) using the auxin repressed protein gene

  • Kim, Jong-Hak (Department of Oriental Medicinal Material & Processing, College of Life Sciences, Kyung Hee University) ;
  • Kim, Min-Kyeoung (KM Fundamental Research Division, Korea Institute of Oriental Medicine) ;
  • Wang, Hongtao (Department of Oriental Medicinal Material & Processing, College of Life Sciences, Kyung Hee University) ;
  • Lee, Hee-Nyeong (Department of Oriental Medicinal Material & Processing, College of Life Sciences, Kyung Hee University) ;
  • Jin, Chi-Gyu (Department of Oriental Medicinal Material & Processing, College of Life Sciences, Kyung Hee University) ;
  • Kwon, Woo-Saeng (Department of Oriental Medicinal Material & Processing, College of Life Sciences, Kyung Hee University) ;
  • Yang, Deok-Chun (Department of Oriental Medicinal Material & Processing, College of Life Sciences, Kyung Hee University)
  • Received : 2015.01.23
  • Accepted : 2015.12.03
  • Published : 2016.10.15
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Abstract

Background: Korean ginseng (Panax ginseng) is one of the most important medicinal plants in the Orient. Among nine cultivars of P. ginseng, Chunpoong commands a much greater market value and has been planted widely in Korea. Chunpoong has superior quality "Chunsam" ($1^{st}$ grade ginseng) when made into red ginseng. Methods: A rapid and reliable method for discriminating the Chunpoong cultivar was developed by exploiting a single nucleotide polymorphism (SNP) in the auxin repressed protein gene of nine Korean ginseng cultivars using specific primers. Results: An SNP was detected between Chunpoong and other cultivars, and modified allele-specific primers were designed from this SNP site to specifically identify the Chunpoong cultivar and P. quinquefolius via multiplex polymerase chain reaction (PCR). Conclusion: These results suggest that great impact to prevent authentication of precise Chunpoong and other cultivars using the auxin repressed protein gene. We therefore present an effective method for the authentication of the Chunpoong cultivar of P. ginseng and P. quinquefolius.

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References

  1. Liu CX, Xiao PG. Recent advances in ginseng research in China. J Ethnopharmacol 1992;36:27-38. https://doi.org/10.1016/0378-8741(92)90057-X
  2. Lee FC. Facts about ginseng, the elixir of life. In: Elizabeth NJ, editor. Elizabeth, New Jersey, U.S.A.: Hollym International Corporation; 1992.
  3. Wang LC, Lee TF. Effect of ginseng saponins on cold tolerance in young and elderly rats. Planta Med 2000;66:144-7. https://doi.org/10.1055/s-2000-11122
  4. Chen XC, Zhou YC, Chen Y, Zhu YG, Fang F, Chen LM. Ginsenoside Rg1 reduces MPTP-induced substantia nigra neuron loss by suppressing oxidative stress. Acta Pharmacol Sin 2005;26:56-62. https://doi.org/10.1111/j.1745-7254.2005.00019.x
  5. Choi KT. Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C.A. Meyer. Acta Pharmacol Sin 2008;29:1109-18. https://doi.org/10.1111/j.1745-7254.2008.00869.x
  6. Xie JT, Mehendale SR, Li X, Quigg R, Wang X, Wang CZ, Wu JA, Aung HH, A Rue P, Bell GI, et al. Anti-diabetic effect of ginsenoside Re in ob/ob mice. Biochim Biophys Acta 2004;1740:319-25.
  7. Joo SS, Won TJ, Lee DI. Reciprocal activity of ginsenosides in the production of proinflammatory repertoire, and their potential roles in neuroprotection in vivo. Planta Med 2005;71:476-81. https://doi.org/10.1055/s-2005-864145
  8. Kwon WS, Chung CM, Kim YT, Lee MG, Choi KT. Breeding process and characteristics of KG101, a superior line of Panax ginseng C.A. Meyer. J Ginseng Res 1998;22:11-7.
  9. Wang TS, Wang YP. New cultivars and rotation system of Korean ginseng. J Ginseng Res 2003;3:13-4.
  10. Chen CF, Chiou WF, Zhang JT. Comparison of the pharmacological effects of Panax ginseng and Panax quinquefolium. Acta Pharmacol Sin 2008;29:1103-8. https://doi.org/10.1111/j.1745-7254.2008.00868.x
  11. Attele AS, Wu JA, Yuan CS. Ginseng pharmacology: multiple constituents and multiple actions. Biochem Pharmacol 1999;58:1685-93. https://doi.org/10.1016/S0006-2952(99)00212-9
  12. Sun H, Wang HT, Kwon WS, In JG, Lee BS, Yang DC. Development of molecular markers for new cultivar 'Chunpoong' in Panax ginseng C. A. Meyer associated with a Major Latex-like protein gene. Biol Pharm Bull 2010;33:183-7. https://doi.org/10.1248/bpb.33.183
  13. Weiskirchen R, Siemeister G, Hartl M, Bister K. Sequence and expression of a glyceraldehyde-3-phosphate dehydrogenase-encoding gene from quail embryo fibroblasts. Gene 1993;128:269-72. https://doi.org/10.1016/0378-1119(93)90573-L
  14. Ulmasov T, Murfett J, Hagen G, Guilfoyle TJ. Aux/lAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin. Plant Cell 2007;9:1963-71.
  15. Abel S, Theologis A. Early genes and auxin action. Plant Physiol 1996;111:9-17. https://doi.org/10.1104/pp.111.1.9
  16. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT Nucleic. Acids Symp Ser 1999;41:95-8.
  17. Yip TT, Lau CN, But PP, Kong YC. Quantitative analysis of ginsenosides in fresh Panax ginseng. Am J Chinese Med 1985;13:77-88. https://doi.org/10.1142/S0192415X85000125
  18. Kim NH, Choi HI, Kim KH, Jang WJ, Yang TJ. Evidence of genome duplication revealed by sequence analysis of multi-loci expressed sequence tag-simple sequence repeat bands in Panax ginseng Meyer. J Ginseng Res 2014;38:130-5. https://doi.org/10.1016/j.jgr.2013.12.005
  19. Choi HI, Kim NH, Kim JH, Choi BS, Ahn IO, Lee JS, Yang TJ. Development of reproducible EST-derived SSR markers and assessment of genetic diversity in Panax ginseng cultivars and related species. J Ginseng Res 2011;35:399-412. https://doi.org/10.5142/jgr.2011.35.4.399
  20. Choi HI, Kim NH, Lee JK, Choi BS, Kim KD, Park JY, Lee SC, Yang TJ. Evolutionary relationship of Panax ginseng and P. quinquefolius inferred from sequencing and comparative analysis of expressed sequence tags. Genet Resour Crop Evol 2013;60:1377-87. https://doi.org/10.1007/s10722-012-9926-3

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