한국어류학회지 (Korean Journal of Ichthyology)

  • 제28권3호
  • /
  • Pages.141-146
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  • 2016
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  • 1225-8598(pISSN)
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  • 2288-3371(eISSN)
한국어류학회 (The Ichthyological Society of Korea)
권호 표지

넙치 (Paralichtys olivaceus) 유래 viral hemorrhagic septicemia virus (VHSV, genotype IVa)의 무지개송어 (Oncorhynchus mykiss) 감염 실험

Experimental Infection of Rainbow Trout (Oncorhynchus mykiss) with Viral Hemorrhagic Septicemia Virus (VHSV, Genotype IVa) from Olive Flounder (Paralichtys olivaceus)

  • 김위식 (전남대학교 수산생명의학과) ;
  • 정하나 (전남대학교 수산생명의학과) ;
  • 공경희 (전남대학교 수산생명의학과) ;
  • 김아라 (전라남도 해양수산과학원) ;
  • 전영호 (전라남도 해양수산과학원) ;
  • 오명주 (전남대학교 수산생명의학과)
  • Kim, Wi-Sik (Department of Aqualife Medicine, Chonnam National University) ;
  • Jeong, Ha-Na (Department of Aqualife Medicine, Chonnam National University) ;
  • Kong, Kyoung-hui (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Ah-ra (Jeollanam-do Ocean Fishery Science Institute) ;
  • Jeon, Young-Ho (Jeollanam-do Ocean Fishery Science Institute) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University)
  • 투고 : 2016.06.27
  • 심사 : 2016.09.21
  • 발행 : 2016.09.30
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초록

본 연구에서는 넙치 유래 VHSV (genotype IVa)에 의한 무지개송어의 영향을 조사하기 위하여 다양한 조건에서 감염 실험을 실시하였다. 3개의 다른 로트의 담수 무지개송어 치어에 VHSV ($10^{6.3-7.3}TCID_{50}$/fish)를 복강 주사한 결과, 15% 이하의 누적 폐사율이 관찰되었다. 무지개송어 (in vivo)를 사용하여 VHSV를 계대 배양한 후 감염 실험을 실시한 결과, VHSV를 5회 계대 배양하여도 독력의 변화는 관찰되지 않았다. 해수에 사육 중인 송어 (성어)에 넙치 유래 VHSV ($10^{5.3}TCID_{50}$/fish와 $10^{6.3}TCID_{50}$/fish)를 복강 주사한 결과, 폐사어는 관찰되지 않았고, 어체 내에서도 VHSV는 검출되지 않았다. 이상의 결과, 해수 송어 (성어)에서는 넙치 유래 VHSV의 병원성이 확인되지 않았으나 담수 무지개송어 치어에서는 낮은 독력을 나타내었다.

Experimental infection of rainbow trout Oncorhynchus mykiss with viral hemorrhagic septicemia virus (VHSV, genotype IVa) from olive flounder Paralichtys olivaceus was examined. The cumulative mortalities of three different lot of rainbow trout fry challenged with VHSV ($10^{6.3-7.3}TCID_{50}$/fish) were less than 15%. No difference of virulence was observed in experimental infection using 5 in vivo passaged VHSV and original VHSV. No mortality was observed in seawater-reared rainbow trout (adult) challenged with VHSV ($10^{5.3-6.3}TCID_{50}$/fish) and virus was not detected in the fish. We thus concluded that VHSV from olive flounder has low virulence to rainbow trout fry, but not pathogenic to seawater-rainbow trout (adult).

키워드

참고문헌

  1. Chun, S.K. 2000. Aquaculture disease-marine fish. Korea Fisheries Publishing Co., 12pp. (in Korean)
  2. Dale, O.B., I. Orpetveit, T.M. Lyngstad, S. Kahns, H.F. Skall, N.J. Olesen and B.H. Dannevig. 2009. Outbreak of viral hemorrhagic septicemia (VHS) in seawater-farmed rainbow trout in Norway caused by VHS virus Genotype III. Dis. Aquatic. Org., 85: 93-103. https://doi.org/10.3354/dao02065
  3. Egusa, S., H. Wakabayashi and K. Muroga. 2006. Infectious and parasitic diseases of fish and shellfish. Life Science Publishing Co. Seoul, pp. 54-58. (in Korean)
  4. Gagne, N., A.M. MacKinnon, L. Boston, B. Souter, M. Cook-Versloot, S. Griffiths and G. Olivier. 2007. Isolation of viral haemorrhagic septicaemia virus from mummichog, stickleback, striped bass and brown trout in eastern Canada. J. Fish Dis., 30: 213-223. https://doi.org/10.1111/j.1365-2761.2007.00802.x
  5. Kim, S.M., J.I. Lee, M.J. Hong, H.S. Park and S.I. Park. 2003. Genetic relationship of the VHSV (viral hemorrhagic septicemia virus) isolated from cultured olive flounder, Paralichthys olivaceus in Korea. J. Fish Pathol., 16: 1-12. (in Korean)
  6. Kim, W.S., H.N. Ok, D.H. Kim, H.Y. Kim and M.J. Oh. 2011a. Current status of pathogen infection in cultured eel Anguilla japonica between 2000 and 2010. J. Fish Pathol., 24: 237-245. (in Korean) https://doi.org/10.7847/jfp.2011.24.3.237
  7. Kim, W.S., J.O. Kim, J.K. Cho and M.J. Oh. 2014. Change of viral hemorrhagic septicemia virus (VHSV) titer in olive flounder (Paralichthys olivaceus) following Poly (I:C) administration. Aquacult. Int., 22: 1175-1179. https://doi.org/10.1007/s10499-013-9737-3
  8. Kim, W.S., S.J. Jung, J.O. Kim, D.W. Kim, J.H. Kim and M.J. Oh. 2011b. Genetic positioning of Korean viral hemorrhagic septicemia virus (VHSV) from cultured and wild marine fishes. J. Fish Pathol., 24: 1-9. https://doi.org/10.7847/jfp.2011.24.1.001
  9. Kim, W.S., S.R. Kim, D.W. Kim, J.O. Kim, M.A. Park, S.I. Kitamura, H.Y. Kim, D.H. Kim, H.J. Han, S.J. Jung and M.J. Oh. 2009. An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea. Aquaculture, 296: 165-168. https://doi.org/10.1016/j.aquaculture.2009.07.019
  10. Kim, W.S., T. Nishizawa, J.H. Kim, R. Suebsing, S.J. Jung and M.J. Oh. 2011c. Korean and Japanese isolates of viral hemorrhagic septicemia virus from olive flounder are pathogenic to rainbow trout fry. Fish Pathol., 46: 112-115. https://doi.org/10.3147/jsfp.46.112
  11. LaPatra, S.E. 1998. Factors affecting pathogenicity of infectious hematopoietic necrosis virus (IHNV) for salmonid fish. J. Aquat. Anim. Health, 10: 121-131. https://doi.org/10.1577/1548-8667(1998)010<0121:FAPOIH>2.0.CO;2
  12. Lee, N.S. 2013. The current situation and suggestions of the mariculture of rainbow trout in Korea. Oceans and Fisheries, 3: 86-112. (in Korean)
  13. Mahy, B.W.J. and H.O. Kangro. 1996. Virology methods manual. Academy Press, London, pp. 35-46.
  14. Nishizawa, T., H. Iida, R. Takano, T. Isshiki, K. Nakajima and K. Muroga. 2002. Genetic relatedness among Japanese, American and European isolates of viral hemorrhagic septicemia virus (VHSV) based on partial G and P genes. Dis. Aquat. Org., 48: 143-148. https://doi.org/10.3354/dao048143
  15. Skall, H.F., N.J. Olesen and S. Mellergaard. 2005. Viral haemorrhagic septicemia virus in marine fish and its implications for fish farming-a review. J. Fish Dis., 28: 509-529. https://doi.org/10.1111/j.1365-2761.2005.00654.x
  16. Skall, H.F., W.J. Slierendrecht, J.A. King and N.J. Olesen. 2004. Experimental infection of rainbow trout Onchorhynchus mykiss with viral haemorrhagic septicemia virus isolates from European marine and farmed fishes. Dis. Aquatic. Org., 58: 99-110. https://doi.org/10.3354/dao058099
  17. Snieszko, S.F. 1974. The effects of environmental stress on outbreaks of infectious diseases of fishes. J. Fish Biol., 6: 197-208. https://doi.org/10.1111/j.1095-8649.1974.tb04537.x
  18. Snow, M. and C.O. Cunningham. 2000. Virulence and nucleotide sequence analysis of marine viral haemorrhagic septicemia virus following in vivo passage in rainbow trout Onchorhynchus mykiss. Dis. Aquatic. Org., 42: 17-26. https://doi.org/10.3354/dao042017
  19. Snow, M., C.O. Cunningham, W.T. Melvin and G. Kurath. 1999. Analysis of the nucleoprotein gene identifies distinct lineages of viral haemorrhagic septicaemia virus within the European marine environment. Virus Res., 63: 35-44. https://doi.org/10.1016/S0168-1702(99)00056-8
  20. Stone, D.M., K. Way and P.F. Dixon. 1997. Nucleotide sequence of the glycoprotein gene of viral haemorrhagic septicaemia (VHS) viruses from different geographic areas: a link between VHS in farmed fish species and viruses isolated from North Sea cod (Gadus morhua L.). J. Gen. Virol., 78: 1319-1326. https://doi.org/10.1099/0022-1317-78-6-1319
  21. Trdo, N., A. Benmansour, C. Calisher, R.G. Dietzgen, R.X. Fang, A.O. Jackson, G. Kurath, S. Nadin-Davis, R.B. Tesh and P.J. Walker. 2005. Family Rhabdoviridae. In: Fauquet, C.M., M.A. Mayo, J. Maniloff, U. Desselberger and L.A. Ball (eds.), Virus Taxonomy: Eight Report of the International Committee on Taxonomy of Viruses. Elsevier/Academic Press, London, pp. 623-653.
  22. Wolf, K. 1988. Viral hemorrhagic septicemia virus. In: Wolf K (ed.) Fish viruses and fish viral diseases. Cornell University Press, Ithaca, pp. 217-249.