Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Experimental Infection of Rainbow Trout (Oncorhynchus mykiss) with Viral Hemorrhagic Septicemia Virus (VHSV, Genotype IVa) from Olive Flounder (Paralichtys olivaceus)

넙치 (Paralichtys olivaceus) 유래 viral hemorrhagic septicemia virus (VHSV, genotype IVa)의 무지개송어 (Oncorhynchus mykiss) 감염 실험

  • Kim, Wi-Sik (Department of Aqualife Medicine, Chonnam National University) ;
  • Jeong, Ha-Na (Department of Aqualife Medicine, Chonnam National University) ;
  • Kong, Kyoung-hui (Department of Aqualife Medicine, Chonnam National University) ;
  • Kim, Ah-ra (Jeollanam-do Ocean Fishery Science Institute) ;
  • Jeon, Young-Ho (Jeollanam-do Ocean Fishery Science Institute) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, Chonnam National University)
  • 김위식 (전남대학교 수산생명의학과) ;
  • 정하나 (전남대학교 수산생명의학과) ;
  • 공경희 (전남대학교 수산생명의학과) ;
  • 김아라 (전라남도 해양수산과학원) ;
  • 전영호 (전라남도 해양수산과학원) ;
  • 오명주 (전남대학교 수산생명의학과)
  • Received : 2016.06.27
  • Accepted : 2016.09.21
  • Published : 2016.09.30
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Abstract

Experimental infection of rainbow trout Oncorhynchus mykiss with viral hemorrhagic septicemia virus (VHSV, genotype IVa) from olive flounder Paralichtys olivaceus was examined. The cumulative mortalities of three different lot of rainbow trout fry challenged with VHSV ($10^{6.3-7.3}TCID_{50}$/fish) were less than 15%. No difference of virulence was observed in experimental infection using 5 in vivo passaged VHSV and original VHSV. No mortality was observed in seawater-reared rainbow trout (adult) challenged with VHSV ($10^{5.3-6.3}TCID_{50}$/fish) and virus was not detected in the fish. We thus concluded that VHSV from olive flounder has low virulence to rainbow trout fry, but not pathogenic to seawater-rainbow trout (adult).

본 연구에서는 넙치 유래 VHSV (genotype IVa)에 의한 무지개송어의 영향을 조사하기 위하여 다양한 조건에서 감염 실험을 실시하였다. 3개의 다른 로트의 담수 무지개송어 치어에 VHSV ($10^{6.3-7.3}TCID_{50}$/fish)를 복강 주사한 결과, 15% 이하의 누적 폐사율이 관찰되었다. 무지개송어 (in vivo)를 사용하여 VHSV를 계대 배양한 후 감염 실험을 실시한 결과, VHSV를 5회 계대 배양하여도 독력의 변화는 관찰되지 않았다. 해수에 사육 중인 송어 (성어)에 넙치 유래 VHSV ($10^{5.3}TCID_{50}$/fish와 $10^{6.3}TCID_{50}$/fish)를 복강 주사한 결과, 폐사어는 관찰되지 않았고, 어체 내에서도 VHSV는 검출되지 않았다. 이상의 결과, 해수 송어 (성어)에서는 넙치 유래 VHSV의 병원성이 확인되지 않았으나 담수 무지개송어 치어에서는 낮은 독력을 나타내었다.

Keywords

References

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