Mycobiology

  • 제44권3호
  • /
  • Pages.121-130
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  • 2016
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  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
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Unique Phylogenetic Lineage Found in the Fusarium-like Clade after Re-examining BCCM/IHEM Fungal Culture Collection Material

  • Triest, David (BCCM/IHEM Collection of Biomedical Fungi, Service of Mycology & Aerobiology, Scientific Institute of Public Health) ;
  • De Cremer, Koen (Service of Health & Environment, Scientific Institute of Public Health) ;
  • Pierard, Denis (Department of Microbiology & Infection Control, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel) ;
  • Hendrickx, Marijke (BCCM/IHEM Collection of Biomedical Fungi, Service of Mycology & Aerobiology, Scientific Institute of Public Health)
  • 투고 : 2016.03.29
  • 심사 : 2016.07.12
  • 발행 : 2016.09.30
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초록

Recently, the Fusarium genus has been narrowed based upon phylogenetic analyses and a Fusarium-like clade was adopted. The few species of the Fusarium-like clade were moved to new, re-installed or existing genera or provisionally retained as "Fusarium." Only a limited number of reference strains and DNA marker sequences are available for this clade and not much is known about its actual species diversity. Here, we report six strains, preserved by the Belgian fungal culture collection BCCM/IHEM as a Fusarium species, that belong to the Fusarium-like clade. They showed a slow growth and produced pionnotes, typical morphological characteristics of many Fusarium-like species. Multilocus sequencing with comparative sequence analyses in GenBank and phylogenetic analyses, using reference sequences of type material, confirmed that they were indeed member of the Fusarium-like clade. One strain was identified as "Fusarium" ciliatum whereas another strain was identified as Fusicolla merismoides. The four remaining strains were shown to represent a unique phylogenetic lineage in the Fusarium-like clade and were also found morphologically distinct from other members of the Fusarium-like clade. Based upon phylogenetic considerations, a new genus, Pseudofusicolla gen. nov., and a new species, Pseudofusicolla belgica sp. nov., were installed for this lineage. A formal description is provided in this study. Additional sampling will be required to gather isolates other than the historical strains presented in the present study as well as to further reveal the actual species diversity in the Fusarium-like clade.

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참고문헌

  1. Gräfenhan T, Schroers HJ, Nirenberg HI, Seifert KA. An overview of the taxonomy, phylogeny, and typification of nectriaceous fungi in Cosmospora, Acremonium, Fusarium, Stilbella, and Volutella. Stud Mycol 2011;68:79-113. https://doi.org/10.3114/sim.2011.68.04
  2. Geiser DM, Aoki T, Bacon CW, Baker SE, Bhattacharyya MK, Brandt ME, Brown DW, Burgess LW, Chulze S, Coleman JJ, et al. One fungus, one name: defining the genus Fusarium in a scientifically robust way that preserves the longstanding use. Phytopathology 2013;103:400-8. https://doi.org/10.1094/PHYTO-07-12-0150-LE
  3. Lombard L, van der Merwe NA, Groenewald JZ, Crous PW. Generic concepts in Nectriaceae. Stud Mycol 2015;80:189-245. https://doi.org/10.1016/j.simyco.2014.12.002
  4. O'Donnell K, Rooney AP, Proctor RH, Brown DW, McCormick SP, Ward TJ, Frandsen RJ, Lysoe E, Rehner SA, Aoki T, et al. Phylogenetic analyses of RPB1 and RPB2 support a middle Cretaceous origin for a clade comprising all agriculturally and medically important fusaria. Fungal Genet Biol 2013;52:20-31. https://doi.org/10.1016/j.fgb.2012.12.004
  5. Bills GF, Platas G, Overy DP, Collado J, Fillola A, Jiménez MR, Martín J, del Val AG, Vicente F, Tormo JR, et al. Discovery of the parnafungins, antifungal metabolites that inhibit mRNA polyadenylation, from the Fusarium larvarum complex and other Hypocrealean fungi. Mycologia 2009;101:449-72. https://doi.org/10.3852/08-163
  6. Triest D, Stubbe D, De Cremer K, Piérard D, Normand AC, Piarroux R, Detandt M, Hendrickx M. Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of molds of the Fusarium genus. J Clin Microbiol 2015;53:465-76. https://doi.org/10.1128/JCM.02213-14
  7. Hosoya T, Tubaki K. Fusarium matuoi sp. nov. and its teleomorph Cosmospora matuoi sp. nov. Mycoscience 2004;45:261-70. https://doi.org/10.1007/S10267-004-0182-Z
  8. Leslie JF, Summerell BA, Bullock S. The Fusarium laboratory manual. Hoboken (NJ): Wiley-Blackwell; 2006.
  9. Beguin H, Pyck N, Hendrickx M, Planard C, Stubbe D, Detandt M. The taxonomic status of Trichophyton quinckeanum and T. interdigitale revisited: a multigene phylogenetic approach. Med Mycol 2012;50:871-82. https://doi.org/10.3109/13693786.2012.684153
  10. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. London: Academic Press; 1990. p. 315-22.
  11. Hopple JS Jr, Vilgalys R. Phylogenetic relationships in the mushroom genus Coprinus and dark-spored allies based on sequence data from the nuclear gene coding for the large ribosomal subunit RNA: divergent domains, outgroups, and monophyly. Mol Phylogenet Evol 1999;13:1-19. https://doi.org/10.1006/mpev.1999.0634
  12. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 1999;91:553-6. https://doi.org/10.2307/3761358
  13. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995;61:1323-30.
  14. Van Hove F, Waalwijk C, Logrieco A, Munaut F, Moretti A. Gibberella musae (Fusarium musae) sp. nov., a recently discovered species from banana is sister to F. verticillioides. Mycologia 2011;103:570-85. https://doi.org/10.3852/10-038
  15. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 2007;24:1596-9. https://doi.org/10.1093/molbev/msm092
  16. Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 2012;61:539-42. https://doi.org/10.1093/sysbio/sys029
  17. Grum-Grzhimaylo AA, Georgieva ML, Debets AJ, Bilanenko EN. Are alkalitolerant fungi of the Emericellopsis lineage (Bionectriaceae) of marine origin? IMA Fungus 2013;4:213-28. https://doi.org/10.5598/imafungus.2013.04.02.07
  18. Rambaut A, Suchard M, Drummond A. Tracer v1.5 software [Internet]. Andrew Rambaut; 2007 [cited 2016 May 2]. Available from: http://tree.bio.ed.ac.uk/software/tracer/.
  19. Booth C. The genus Fusarium. Kew: Commonwealth Mycological Institute; 1971.