The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Korean Tricholoma matsutake Strains that Promote Mycorrhization and Growth of Pinus densiflora Seedlings

균근 형성과 소나무 유묘 생장이 우수한 송이 균주의 선발

  • Jeon, Sung-Min (Department of Life Science, Sangmyung University) ;
  • Ka, Kang-Hyeon (Division of Wood Chemistry and Microbiology, National Institute of Forest Science)
  • 전성민 (상명대학교 생명과학과) ;
  • 가강현 (국립산림과학원 화학미생물과)
  • Received : 2015.08.18
  • Accepted : 2016.09.12
  • Published : 2016.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Domestic and international production of Tricholoma matsutake has decreased owing to matsutake forests being left alone, host plant disease, forest fires, climate change, and so on. In order to identify strains that are suitable for the production of T. matsutake-inoculated seedlings, Pinus densiflora seedlings were inoculated with T. matsutake after in vitro rooting and mycorrhization was examined in the roots of T. matsutake-inoculated seedlings after 6 months. The mycorrhization rate was greater than 80% for 5 strains (NIFoS 421, 434, 1681, 1984, and 2001) out of 19 total strains. Seven strains (NIFoS 434, 441, 561, 562, 1016, 1807, and 1812) showed shoot/root ratios of less than 3.0 and had a seedling shoot biomass of 2.0 to 4.8 times higher than that of the root. Eight strains (NIFoS 441, 561, 562, 1016, 1807, 1812, 1984, and 2001) stimulated increases in shoot volume and three stains (NIFoS 441, 562, and 1812) promoted the growth of root biomass by mycorrhizal formation. In conclusion, 4 strains (NIFoS 434, 561, 1984, and 2001) out of 19 total strains tested showed higher mycorrhization rates and seedling growth than those of the other strains. We expect that the use of these four strains may contribute to T. matsutake-inoculated seedling production.

송이산의 방치, 기주식물의 질병, 산불, 기후변화 등 다양한 원인에 의해 국내외 송이 생산량이 감소하고 있어 송이균의 생태적 특성을 이용한 인공재배 기술 개발이 필요한 실정이다. 본 연구에서는 송이 접종묘 생산에 적합한 균주를 선발하기 위해 무균 배양병 내에서 소나무 유묘와 송이균 간의 외생균근 합성을 시도하였다. 송이 균근 형성률은 총 19개 균주 중 5개 균주(NIFoS 421, 434, 1681, 1984, 2001)가 80% 이상을 나타냈다. 7개 송이 균주(NIFoS 434, 441, 561, 562, 1016, 1807, 1812)는 평균 3.0 이하의 S/R ratio를 나타내었고, 소나무 유묘 지상부의 생물량이 지하부보다 2.0~4.8배 정도 높았다. 8개 균주(NIFoS 441, 561, 562, 1016, 1807, 1812, 1984, 2001)는 유묘의 지상부 부피 생장에, 3개 균주(NIFoS 441, 562, 1812)는 균근을 형성하면서 지하부 부피 생장을 돕는 것을 알 수 있었다. 결론적으로 시험균을 접종한 지 6개월 후, 총 19개 시험균 중 4 균주 (NIFoS 434, 561, 1984, 2001)는 타 균주들에 비해 송이균의 균근 형성력과 소나무 유묘 생장력이 우수한 것으로 나타나 송이 접종묘 제작에 있어 이들의 활용이 기대된다.

Keywords

References

  1. Ka KH, Koo CD. Research questions for artificial cultivation of Tricholoma matsutake. Trends Agric Life Sci 2002;2:1-6.
  2. Zeng YW, Yang JZ, Pu XY, Du J, Yang T, Yang SM, Zhu WH. Strategies of functional food for cancer prevention in human beings. Asian Pac J Cancer Prev 2013;14:1585-92. https://doi.org/10.7314/APJCP.2013.14.3.1585
  3. Kim YE, Yang JW, Lee CH, Kwon EK. ABTS radical scavenging and anti-tumor effects of Tricholoma matsutake Sing. (Pine mushroom). J Korean Soc Food Sci Nutr 2009;38: 555-60. https://doi.org/10.3746/jkfn.2009.38.5.555
  4. Lim HW, Yoon JH, Kim YS, Lee MW, Park SY, Choi HK. Free radical-scavenging and inhibition of nitric oxide production by four grades of pine mushroom (Tricholoma matsutake Sing.) Food Chem 2007;103:1337-42. https://doi.org/10.1016/j.foodchem.2006.10.046
  5. Kim JY, Byeon SE, Lee YG, Lee JY, Park J, Hong EK, Cho JY. Immunostimulatory activities of polysaccharides from liquid culture of pine-mushroom Tricholoma matsutake. J Microbiol Biotechnol 2008;18:95-103.
  6. Choi SY, Kim NN, Kim YE, Lee Y, Kim SJ, Kim JH. Inhibitory effects of cultured Tricholoma matsutake mycelia on melanin biosynthesis. Korean J Food Sci Technol 2011;43:240-2. https://doi.org/10.9721/KJFST.2011.43.2.240
  7. Kim JS, Park JB, Jang SW, Kwon DH, Jang MH, Lee MO, Ha SJ. Determination of antibacterial activity from Tricholoma matsutake extract and its application to low salted Jeot-gal. Korean Soc Biotechnol Bioeng J 2015;30:253-6.
  8. Guerin-Laguette A, Shindo K, Matsushita N, Suzuki K, Lapeyrie F. The mycorrhizal fungus Tricholoma matsutake stimulates Pinus densiflora seedling growth in vitro. Mycorrhiza 2004; 14:397-400. https://doi.org/10.1007/s00572-004-0322-5
  9. Islam F, Ohga S. The response of fruit body formation on Tricholoma matsutake in situ condition by applying electric pulse stimulator. Int Sch Res Notices 2012. http://dx.doi.org/10.5402/2012/462724.
  10. Yamanaka T. Researches for development of the cultivation of 'matsutake', a prized mushroom produced by the ectomycorrhizal basidiomycete Tricholoma matsutake. Bull For For Prod Res Inst 2012;11:85-95.
  11. Ka KH, Park H, Bak WC, Kim HS, Hur TC, Yoon KH, Ryu SR, Lee BH, Koo CD, Lee SI, et al. Artificial production of pinemushroom using transplanted pine trees infected by Tricholoma matsutake. Seoul: Korea Forest Research Institute; 2010.
  12. Korea Forest Service. 2015 Statistical yearbook of forestry. Daejeon: Korea Forest Service; 2015.
  13. National Indicator System of the Statics Korea. Forest damage occurrence and prevention by forest pest insect and disease [Internet]. Daejeon: Statistics Korea; 2016. Available from http://www.index.go.kr/potal/main/EachDtlPageDetail.do?idx _cd=1310.
  14. Ka KH, Kim HS, Hur TC, Park H, Bak WC. Artificial cultivation of Tricholoma matsutake using matsutake-infected pine tree. Seoul: Korea Forest Research Institute; 2009.
  15. Ka KH, Jeon SM, Park H, Lee WY, Oh DS, Choi JW. Development of mushroom cultivation technology on coniferous resources as a medium. Seoul: Korea Forest Research Institute; 2013.
  16. Marx DH. The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria. Phytopathology 1969;59:153-63.
  17. Ka KH, Park H, Hur TC, Bak WC. Selection of ectomycorrhizal isolates of Tricholoma matsutake and Tricholoma magnivelare for inoculation on seedlings of Pinus densiflora in vitro. Kor J Mycol 2008;36:148-52. https://doi.org/10.4489/KJM.2008.36.2.148
  18. Yamada A, Maeda K, Kobayashi H, Murata H. Ectomycorrhizal symbiosis in vitro between Tricholoma matsutake and Pinus densiflora seedlings that resembles naturally occurring 'shiro'. Mycorrhiza 2006;16:111-6. https://doi.org/10.1007/s00572-005-0021-x
  19. Palmen J. Matsutake: mushroom of the year - or millennium? Fungi 2016;8:40-8.
  20. Yamada A, Endo N, Murata H, Ohta A, Fukuda M. Tricholoma matsutake Y1 strain associated with Pinus densiflora shows a gradient of in vitro ectomycorrhizal specificity with Pinaceae and oak hosts. Mycoscience 2014;55:27-34. https://doi.org/10.1016/j.myc.2013.05.004
  21. Haasse DL. Understanding forest seedling quality: measurements and interpretation. Tree Planters' Notes 2008;52:24-30.
  22. Instructions for forest seedling business: Established Rule No. 599 of Korea Forest Service. 2011.
  23. Bernier PY, Lamhamedi MS, Simpson DG. Shoot:Root ratio is of limited use in evaluating the quality of container conifer stock. Tree Planter's Notes 1995;46:102-6.