Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Transcriptome-based identification of water-deficit stress responsive genes in the tea plant, Camellia sinensis

  • Tony, Maritim (Tea Breeding and Genetics Improvement, Kenya Agriculture and Livestock Research Organization-Tea Research Institute) ;
  • Samson, Kamunya (Tea Breeding and Genetics Improvement, Kenya Agriculture and Livestock Research Organization-Tea Research Institute) ;
  • Charles, Mwendia (Department of Biochemistry and Molecular Biology, Egerton University) ;
  • Paul, Mireji (Department of Epidemiology of Microbial Diseases, Yale School of Public Health) ;
  • Richard, Muoki (Tea Breeding and Genetics Improvement, Kenya Agriculture and Livestock Research Organization-Tea Research Institute) ;
  • Mark, Wamalwa (International Livestock Research Institute- Bioscience East and Central Africa) ;
  • Stomeo, Francesca (International Livestock Research Institute- Bioscience East and Central Africa) ;
  • Sarah, Schaack (International Livestock Research Institute- Bioscience East and Central Africa) ;
  • Martina, Kyalo (International Livestock Research Institute- Bioscience East and Central Africa) ;
  • Francis, Wachira (Association for Strengthening Agricultural Research in Eastern and Central Africa)
  • Received : 2016.04.29
  • Accepted : 2016.08.17
  • Published : 2016.09.30
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Abstract

A study aimed at identifying putative drought responsive genes that confer tolerance to water stress deficit in tea plants was conducted in a 'rain-out shelter' using potted plants. Eighteen months old drought tolerant and susceptible tea cultivars were each separately exposed to water stress or control conditions of 18 or 34% soil moisture content, respectively, for three months. After the treatment period, leaves were harvested from each treatment for isolation of RNA and cDNA synthesis. The cDNA libraries were sequenced on Roche 454 high-throughput pyrosequencing platform to produce 232,853 reads. After quality control, the reads were assembled into 460 long transcripts (contigs). The annotated contigs showed similarity with proteins in the Arabidopsis thaliana proteome. Heat shock proteins (HSP70), superoxide dismutase (SOD), catalase (cat), peroxidase (PoX), calmodulinelike protein (Cam7) and galactinol synthase (Gols4) droughtrelated genes were shown to be regulated differently in tea plants exposed to water stress. HSP70 and SOD were highly expressed in the drought tolerant cultivar relative to the susceptible cultivar under drought conditions. The genes and pathways identified suggest efficient regulation leading to active adaptation as a basal defense response against water stress deficit by tea. The knowledge generated can be further utilized to better understand molecular mechanisms underlying stress tolerance in tea.

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References

  1. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: A new generation of protein database search program. Nucleic Acids Research 25:3389-3402 https://doi.org/10.1093/nar/25.17.3389
  2. Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Critical Reviews in Plant Science 24:23-58 https://doi.org/10.1080/07352680590910410
  3. Bezbaruah HP (1971) Cytological investigation in the family Theacea - I. Chromosome numbers in some Camellia species and allied genera. Carylogia 24:421-426 https://doi.org/10.1080/00087114.1971.10796449
  4. Bogeat-Tribo L, Brosche M, Renaut J, Jouve L, Thiec DL, Fayyaz P, Vinocur B, Witters E, laukens K, Teichmann T, Altman A, Hausman JF, Polle A, Kangasjarvi J, Dreyer E (2007) Gradual soil depletion results in reversible changes of gene expression, protein profiles, ecophysiology and growth perfomance in Popolus euphratica, a poplar growing in arid regions. Plant Physiology 143:876-892
  5. Cabrera C, Gimenez R, Lopez MC (2003) Determination of tea components with antioxidant activity. Agricultural and Food Chemistry 51:4427-4435 https://doi.org/10.1021/jf0300801
  6. Carr MKV (1972) The climatic requirements of tea. A review. Experimental Agriculture 8:1-14 https://doi.org/10.1017/S0014479700023449
  7. Chatzidimitriadou K, Nianiou-Obeedat I, Madesis P, Perl-Treves R, Tsaftari A (2009) Expression of SOD transgene in pepper confer stress tolerance and improve shoot regeneration. Electronic Journal of Biotechnology 12:1-9
  8. Chavez MM, Maroco JP, Pereira JS (2003) Understanding plant response to drought-from genes to the whole plant. Functional Plant Biology 30:239-264 https://doi.org/10.1071/FP02076
  9. Cheruiyot EK, Mumera LM, Ng'etich WK, Hassanali A, Wachira F (2007) Polyphenols as potential indicators for drought tolerance in Tea (Camellia sinensis L. O kuntze). Biosciences Biotechnology Biochemistry 71:2190-2197 https://doi.org/10.1271/bbb.70156
  10. Cheruiyot EK, Mumera LM, Ngetich WK, Hassanali A, Wachira FN (2008) Threshold soil water content for growth of tea (Camellia sinensis (L.) O. Kuntze). Tea 29:30-38
  11. Conesa Gotz S, Garcia-Gomez JM, Terol J, Talon MR (2005) Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics 21:3674-3676 https://doi.org/10.1093/bioinformatics/bti610
  12. Das A, Das S, Mondal TK (2012) Identification of differentially expressed gene profiles in young roots of tea (Camellia sinensis (L.) O. Kuntze) subjected to drought stress using suppression subtractive hybridization. Plant Molecular Biology 30:1088-1101 https://doi.org/10.1007/s11105-012-0422-x
  13. Frei B, Higdon JV (2003) Antioxidant activity of tea polyphenols in vivo: Evidence from animal studies. Journal of Nutrition 133:3275S-3284S https://doi.org/10.1093/jn/133.10.3275S
  14. Gupta S, Bharalee R, Bhorali P, Agarwal N, Ahmed P, Saikia H, Borchetia S, Kalita MS, Handique AK, Das S (2012) Identification of drought tolerant progenies in tea by gene expression analysis. Functional Intergrative Genomics 12:543-563 https://doi.org/10.1007/s10142-012-0277-0
  15. Hackett CA, Wachira FN, Paul S, Powell W, Waugh R (2000) Construction of a genetic linkage map for Camellia sinensis (tea). Heredity 85:346-355 https://doi.org/10.1046/j.1365-2540.2000.00769.x
  16. Heath LS, Ramakrishnam N, Sederoff RR, Whetten RW, Chevone BL, Struble CA, Jouenne VY, Chen D, Zyl L, Green R (2002) Studying the functional genomics of the stress responses in Loblolly pine with the expression microarray experiment management system. Comprehensive Functional Genomics 3:226-243 https://doi.org/10.1002/cfg.169
  17. Kamunya SM, Wachira FN, Pathak RS, Muoki RC, Wanyoko JK, Ronno WK, Sharma RK (2009) Quantitative genetic parameters in tea (Camellia sinensis (L.) O. Kuntze): I Combining abilities for yield, drought tolerance and quality traits. African Journal of Science 3:93-101
  18. Kamunya SM, Wachira FN, Pathak RS, Korir R, Sharma V, Kumar R, Bhardwaj P, Chalo R, Ahuja PS, Sharma RK (2010) Genomic mapping and testing for quantitative trait loci in tea (Camellia sinensis (L.) O. Kuntze). Tree Genetics and Genomes 6:915-926 https://doi.org/10.1007/s11295-010-0301-2
  19. Karori SM, Wachira FN, Wanyoko JK, Ngure RM (2007) Antioxidant capacity of different types of tea products. African Journal of Biotechnology 6:2287-2296 https://doi.org/10.5897/AJB2007.000-2358
  20. Kondo K (1977) Chromosome numbers in the genus Camellia. Biotropica 9:86-94 https://doi.org/10.2307/2387663
  21. Lin YS, Tsai YJ, Tsay JS, Lin JK (2003) Factors affecting the levels of tea polyphenols and caffeine in tea leave. Journal of Agricultural Food Chemistry 51:1864-1873 https://doi.org/10.1021/jf021066b
  22. Maritim TK, Kamunya SM, Mireji P, Mwendia V, Muoki RC, Cheruiyot EK and Wachira FN (2015) Physiological and biochemical responses of tea (Camellia sinensis L. O Kuntze) to water-deficit stress. Journal of Horticultural Science & Biotechnology 90(4):395-400 https://doi.org/10.1080/14620316.2015.11513200
  23. Mohammad IA, Lin TP (2010) Molecular analysis of dehydration in plants. Plant Science 1:21-25
  24. Mphangwe NI, Vorster J, Steyn M, Nyirenda HE, Taylor NJ, Apostolides Z (2013) Screening of tea (Camellia sinensis) for traits associated Molecular markers. Applied Biochemical Biotechnology 171:437-449 https://doi.org/10.1007/s12010-013-0370-4
  25. Munnik T, Meijer H (2001) Osmotic stress activate distinct lipid and MAPK signalling pathway in plants. FEBS Lett 498
  26. Muoki RC, Paul A, Kumar S (2012) A shared response of thaumatin like protein, chitinase and late embryogenesis abundant protein to environmental stresses in tea (Camellia sinensis (L.) O. Kuntze). Functional Intergrative Genomics 12(3):565-571 https://doi.org/10.1007/s10142-012-0279-y
  27. Ngetich WK, Stephen W, Othieno CO (2001) Responses of tea to environment in Kenya. III. Yield and yield distribution. Experimental Agriculture 37:361-372
  28. Nishizawa A, Yabuta Y, Shigeoka S (2008) Galactinol and raffinose constitute a novel function to protect plants from oxidative damage. Plant Physiology 147:1251-1263 https://doi.org/10.1104/pp.108.122465
  29. Rossel JB, Water PB, Hendrickson L, Chow WS, Poole A, Mullineaux PM, Pogson BJ (2006) A mutation affecting ascorbate peroxidase 2 gene expression reveals a link between responses to high light and drought tolerance. Plant Cell Environment 29:269-281 https://doi.org/10.1111/j.1365-3040.2005.01419.x
  30. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York, USA
  31. Shakeel AA, Xiao-yu X, Long-chang W, Muhammad FS, Chen ML (2011) Morphological, physiological and biochemical responses of plants to drought stress. African Journal of Agricultural Research 6:2026-2032
  32. Shalini KV, Manjunatha S, Lebrun P, Berger A, Baudouin L, Pirany N, Ranganath RM, Prasad TD (2007) Identification of molecular markers associated with mite resistance in Coconut (Cocos nucifera L.). Genome 50:35-42 https://doi.org/10.1139/g06-136
  33. Sharma P, Kumar A (2005) Differential display-mediated identification of three drought-responsive expressed sequence tags in tea (Camellia sinensis (L.) O. Kuntze). Journal of Bioscience 30:231-235 https://doi.org/10.1007/BF02703703
  34. Shinozaki K, Yamaguchi-Shinozaki K, Seki M (2003) Regulatory network of gene expression in the drought and cold stress responses. Current Opinion in Plant Biology 6:410-417 https://doi.org/10.1016/S1369-5266(03)00092-X
  35. Taji T, Ohsumi C, Luchi S, Seki M, Kasuga M, Kobayashi M, Yamaguchi-Shinozaki K, Shinozaki K (2002) Important roles of drought- and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana. Plant Journal 29:417-426 https://doi.org/10.1046/j.0960-7412.2001.01227.x
  36. Tanaka J, Taniguchi F (2006) Estimation of the genome size of tea (Camellia sinensis), Camellia (C. japonica), and their interspecific hybrids by flow cytometry. Journal of the Remote Sensing Society of Japan 101:1-7
  37. Volk GM, Haritatos EE, Turgeon R (2003) Galactinol synthase gene expression in melon. American Society of Horticultural Science 128:8-15
  38. Wachira FN, Ngetich WK (1999) Dry matter production and partition in diploid, triploid and tetraploid tea. Journal of Horticultural Science and Biotechnology 74:507-512 https://doi.org/10.1080/14620316.1999.11511144
  39. Wachira FN, Ronno W (2004) Current research on tea in Kenya. In:. Proceedings of 2004 International Conference on O-Cha (Tea) Science; Shizouka, Japan, 59-65
  40. Wachira FN, Waugh R, Hackett C, Powell W (1995) Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers. Genome 38:201-210 https://doi.org/10.1139/g95-025
  41. Wisniewki M, Bassett C, Norelli J, Macarisin D, Artlip T, Gasic K, Korban S. (2008) Expressed sequence tag analysis of the response of apple (Malus x domestica Royal Gala) to low temperature and water deficit. Physiology Plant 133:298-317 https://doi.org/10.1111/j.1399-3054.2008.01063.x
  42. Wu H, Chen D, Jiaxian L, Yu B, Qiao X, Huang H, He Y (2012) De novo characterization of leaf transcriptome using 454 sequencing and development of EST-SSR markers in tea, (Camelia sinensis). Plant Molecular Biology Report 31(3):524-538 https://doi.org/10.1007/s11105-012-0519-2
  43. Xu ZZ, Zhou GS, Shimizu H (2009) Effects of soil drought with nocturnal warming on leaf stomatal traits and mesophyll cell ultrastructure of a perennial grass. Crop Science 49:1843-1851 https://doi.org/10.2135/cropsci2008.12.0725

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