Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Morphological and cultural characteristics of a novel Phellinus linteus KACC93057P

신규 목질진흙버섯 KACC93057P의 배양적, 형태적 특성

  • Min, Gyeong-Jin (Graduate School of Bio.&Information Technology, Hankyong National University) ;
  • Kwak, A-Min (Graduate School of Bio.&Information Technology, Hankyong National University) ;
  • Seok, Sun-Ja (Agricultural Microbiology Division, National Academy of Agricultural Science (NAAS), Rural Development Administration (RDA)) ;
  • Kang, Hee-Wan (Graduate School of Bio.&Information Technology, Hankyong National University)
  • 민경진 (한경대학교 미래융합기술대학원) ;
  • 곽아민 (한경대학교 미래융합기술대학원) ;
  • 석순자 (국립 농업과학원 농업미생물과) ;
  • 강희완 (한경대학교 미래융합기술대학원)
  • Received : 2016.09.22
  • Accepted : 2016.09.29
  • Published : 2016.09.30
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Abstract

Morphological and culture characteristics of the novel Phellinus linteus variant KACC93057P collected in Korea were characterized in this study. The surface of the was angular, sessile, tough-woody, concentrically zonate, and dark brown in color. Basidiocarppores were circular, with 5-7 pores per mm. The hyphal system was dimitic, and basidiospores were ellipsoid or oval, $4.5-6{\times}4-5$, exhibiting characteristics typical of P. linteus. The optimum temperature for mycelial growth was $25-30^{\circ}C$, and optimal pH for growth was 5-7. The mycelial growth rate of P. linteus KACC93057P was faster than that of other P. baumii isolates. On growth medium, KACC93057P formed aerial mycelia with density higher than that of other isolates. The internal transcribed spacer (ITS)-ribosomal DNA sequences were closely related to the sequences P. linteus complex.

국내에서 분리한 신규 P linteus KACC93057P의 자실체의 형태적 특성을 육안적 으로 관찰한 결과 대가없이 기주부착형으로 자실체표면은 단단한 목질형태 (hardy woody) 로 동심원으로 윤층대 (zonate)와 홈을 형성하고 담갈색을 띠고 있었다. 자실체 뒷면 테두리는 진황색, 내부는 담황갈색을 띠며 현미경 미세구조를 관찰한 결과 관공은 원형또는 각진형이며, 관공구는 5-7 per mm크기로 나타났다. hyphal system은 2분지형이며 포자는 $4.5-6{\times}4-5{\mu}m$크기로 난형또는 유구형 담갈색을 띠어 P linteus와 유사한 특성을 보였다. YGMA배지상에서 KACC93057P는 $25-30^{\circ}C$가 최적온도로 균사체 성장률이 장수상황과 고려상황에 비하여 10-25% 높았으며 균사밀도가 다른 균주보다 현저히 높게 나타났다. ITS-rDNA염기서열비교결과 96% 이상의 상동성으로 I. lonicericola, I. linteus, I. baumii와 근연관계를 형성 하였다.

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References

  1. Chi JH, Ha TM, Kim YH, Rho YD. 1996. Studies on main factors affecting the mycelial growth of Phellinus linteus. Kor J Mycol. 24:214-222.(in Korean)
  2. Daniel S. 2010. Medicinal mushroom Phellinus linteus as an alternative cancer therapy (Review). Experml therapmed 1:407-411
  3. Heo BS, L KS, Park SC, Lee YS. 2004. Cultural conditions for the mycelial growth of Phellinus spp. Kor J Mycol. 32:134-137.(in Korean). https://doi.org/10.4489/KJM.2004.32.2.134
  4. Hong IP, Jung IY, Nam SH, Park JS, Cho JH, Lee MW. 2002. Cultural characteristics of a medicinal mushroom, Phellinus linteus. Mycobiology. 30:208-212. https://doi.org/10.4489/MYCO.2002.30.4.208
  5. Ikekawa T, Nakanishi M, Uehara N, Chihara G. 1968. Antitumor action of some basidomycetes, especially Phellinus linteus. Gann. 59:155-157.
  6. Jeong WJ, Lim YW, Lee JS Jung HS. 2005. Phylogeny of Phellinus and related genera inferred from combined data of ITS and mitochondrial SSU rDNA sequences. J Microbiol Biotechnol. 15:1028-1038.
  7. Kang HW. 2010. Novel Phellinus sp. KACC93057P producing polysaccharide of high immune and antirumor activity. Korean patent 10-1069683.
  8. Kang, HW, Lee MH, Seo GS. 2013. Antioxidant and antiinflammatory effects of Phellinus linteus HN1009K. Kor J Mycol. 41:243-247.(in Korean) https://doi.org/10.4489/KJM.2013.41.4.243
  9. Lee IK, Jung JY, Kim YH, Yun BS. 2009. Phellinins A1 and A2, new styrylpyrones from the culture broth of Phellinus sp. KACC93057P: II. Physicochemical properties and structure elucidation. J. Antibiotics 62:635-637. https://doi.org/10.1038/ja.2009.83
  10. Lee IK, Seo GS, Jeon NB, Kang HW, Yun BS. 2009. Phellinins A1 and A2, new styrylpyrones from the culture broth of Phellinus sp. KACC93057P: I. Fermentation, taxonomy, isolation and biological properties. J Antibiotics. 62:631-634. https://doi.org/10.1038/ja.2009.82
  11. Lim YW, Lee JS. Jung H.S.2003. Type studies on Phellinus baumii and Phellinus linteus. Mycotaxon. 85:201-210.
  12. Parmasto E, Parmasto I. 2001. Phellinus baumii and related species of the Ph. linteus group (Hymenochaetaceae, Hymenomycetes). Folia Cryptog Estonica. 38:53-61.
  13. Teng SC. 1964. Fungi of China. Science Press, Beijing
  14. Tian XM, Yu HY, Zhou LW, Decock C, Vlasak J, Dai YC. 2013. Phylogeny and taxonomy of Inonotus linteus complex. Fungal Div. 58:159-169 https://doi.org/10.1007/s13225-012-0202-9
  15. WU SH, Dai YC, Hattori T, Yu TW, Wang DM Parmasto E, Chan HY, Shih SY. 2012. Species clarification for the medicinally valuable'sanghuang' mushroom. Bot. Studies 53:135-149.
  16. Zhou LW, Vlasak J, Decock C, Assefa A, Sttenlid J, Abate D, Wu SH, Dai YC. 2016. Global diversity and taxonomy of Inonotus linteus complex (Hymenochaetales, Basidiomycota): Sanghuangporus gen. nov., Trropicoporus excentrodendri and T. guanacastensis gen. et spp. nov., and 17 new combination. Fungal diver. 77:335-347 https://doi.org/10.1007/s13225-015-0335-8