Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Radical Scavenging Effect and Neuroprotective Activity from Oxidative Stress of Engelhardtia chrysolepis Leaf

Engelhardtia chrysolepis의 라디칼 소거능 및 신경세포의 산화 스트레스 보호효과

  • Kim, Eun Jung (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Lee, Ah Young (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Choi, Soo Yeon (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Seo, Hye Rin (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University) ;
  • Lee, Young A (Department of Food Science and Nutrition, Catholic University of Daegu) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University)
  • 김은정 (부산대학교 식품영양학과 & 노인생활환경연구소) ;
  • 이아영 (부산대학교 식품영양학과 & 노인생활환경연구소) ;
  • 최수연 (부산대학교 식품영양학과 & 노인생활환경연구소) ;
  • 서혜린 (부산대학교 식품영양학과 & 노인생활환경연구소) ;
  • 이영아 (대구가톨릭대학교 식품영양학과) ;
  • 조은주 (부산대학교 식품영양학과 & 노인생활환경연구소)
  • Received : 2016.05.24
  • Accepted : 2016.07.11
  • Published : 2016.09.30
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Abstract

In this study, the radical scavenging activity and protective effect of ethanol extract from leaf of Engelhardtia chrysolepis HANCE (ECE) against oxidative stress were investigated under in vitro and cellular system. ECE showed strong radical scavenging activities in 1,1-diphenyl-2-picrylhydrazyl, hydroxyl(${\cdot}OH$) and nitric oxide(NO) radical as a concentration-dependent manner. Particularly, strong scavenging activity against the ${\cdot}OH$ and NO radical were observed with the $IC_{50}$ value of $1.30{\mu}g/ml$ and $12.61{\mu}g/ml$, respectively. Furthermore, the cellular oxidative stress was induced by amyloid beta($A{\beta}_{25-35}$) in C6 glial cells. The treatment of $A{\beta}_{25-35}$ to C6 glial cells generated high levels of reactive oxygen species(ROS) and declined cell viability. However, production of ROS was decreased by the treatment of ECE. In addition, the cell viability was significantly increased at each concentration(10, 25, $50{\mu}g/ml$) as dose-dependent manner. The Alzheimer's disease-related protein expressions in $A{\beta}_{25-35}$-treated C6 glial cells were analyzed. The ECE treatment inhibited expression of amyloid precursor protein(APP), C-terminal fragment-${\beta}(CTF-{\beta})$, ${\beta}$-site APP cleaving enzyme(BACE), phosphorylated tau(p-tau) proteins in C6 glial cells induced by $A{\beta}_{25-35}$. The present study indicated that ECE has strong radical scavenging activity and neuroprotective effect through attenuating oxidative stress.

Keywords

References

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