Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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RAW264.7 Cell Activating Glucomannans Extracted from Rhizome of Polygonatum sibiricum

  • Yelithao, Khamphone (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Surayot, Utoomporn (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Lee, Ju Hun (Department of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • You, SangGuan (Department of Marine Food Science and Technology, Gangneung-Wonju National University)
  • Received : 2016.05.31
  • Accepted : 2016.08.21
  • Published : 2016.09.30
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Abstract

Water-soluble polysaccharides isolated from the rhizome of Polygonatum sibiricum and fractionated using ionexchange chromatography were investigated to determine their structure and immunostimulating activity. Crude and fractions ($F_1$ and $F_2$) consisted of carbohydrates (85.1~88.3%) with proteins (4.51~11.9%) and uronic acid (1.79~7.47%), and included different levels of mannose (62.3~76.3%), glucose (15.2~20.3%), galactose (4.35~15.3%), and arabinose (4.00~7.65%). The crude contained two peaks with molecular weights (Mw) of $151{\times}10^3$ and $31.8{\times}10^3$, but $F_1$ and $F_2$ exhibited one major peak with Mw of $103{\times}10^3$ and $628{\times}10^3$, respectively. Little immunostimulatory activity was observed by the crude; however, $F_1$ and $F_2$ significantly activated RAW264.7 cells to release nitric oxide and various cytokines, suggesting they were potent immunostimulators. The backbone of the most immunostimulating fraction ($F_1$) was ($1{\rightarrow}4$)-manno- and ($1{\rightarrow}4$)-gluco-pyranosyl residues with galactose and glucose attached to O-6 of manno-pyranoside.

Keywords

References

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