Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
권호 표지
DOI QR코드

DOI QR Code

Ultrastructure of the Fertilized Egg Envelope from Pseudobagrus fulvidraco, Bagridae, Teleostei

  • Sohn, Joon Hyung (Institute of Lifestyle Medicine, Yonsei University Wonju College of Medicine) ;
  • Kwon, Ohyun (Department of Visual Optics, Division of Health Science, Baekseok University) ;
  • Kim, Dong Heui (Department of Environmental Medical Biology, Yonsei University Wonju College of Medicine)
  • Received : 2016.09.06
  • Accepted : 2016.09.21
  • Published : 2016.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

The ultrastructure of fertilized egg envelope from Pseudobagrus fulvidraco belongs to Bagridae was investigated using light and electron microscopes. The fertilized egg was compressed spherical, light-yellowish, demersal, and adhesive. The size of fertilized egg is about $1.85{\pm}0.13mm$, perivitelline space is not well developed, and there were no appendicular structures on the outer surface of egg envelope and oil droplets in vitelline membrane under light microscope. The micropyle was located in the animal pole of fertilized egg. Adhesive reticular fiber was covered fertilized egg envelope. The thickness of egg envelope was about $3.7{\sim}4.2{\mu}m$, and the egg envelope consisted of two layers: an outer, electron-dense adhesive fibers layer and an simple inner layer with pore. Therefore, the ultrastructure of cross section of the fertilized egg envelope showed species specificity, but studies on the other species belongs to Bagridae were need to get correct information about common traits in family.

Keywords

References

  1. Anderson E (1974) Comparative aspects of the ultrastructure of the female gamete. Int. Rev. Cytol. Suppl. 4, 1-70.
  2. Bell G R, Hoskins G E, and Bagshaw J W (1969) On the structure and enzymatic degradation of the external membrane of the salmon egg. Can. J. Zool. 47, 145-148.
  3. Brivio M F, Bassi R, and Cotelli F (1991) Identification and characterization of the major components of the Oncorhynchus mykiss egg chorion. Mol. Reprod. Dev. 28, 85-93. https://doi.org/10.1002/mrd.1080280114
  4. Brummett A R and Dumont J N (1981) A comparison of chorions from eggs of northern and southern populations of Fundulus heteroclitus. Copeia 3, 607-614.
  5. Cameron I L and Hunter K E (1984) Regulation of the permeability of the medaka fish embryo chorion by exogeneous sodium and calcium ions. J. Exp. Zool. 231, 447-454. https://doi.org/10.1002/jez.1402310320
  6. Cotelli F, Andronico F, Brivio M, and Lamia C L (1988) Structure and composition of the fish chorion. J. Ultrastruct. Mol. Struct. Res. 99, 70-78. https://doi.org/10.1016/0889-1605(88)90034-1
  7. Deung Y K, Kim D H, and Reu D S (2000) Ultrastructure of the fertilized egg envelope from pale chub, Cyprinidae, teleost. Korean J. Electron Microsc. 30, 321-326.
  8. Deung Y K, Reu D S, and Kim D H (1997) Comparative ultrastructures of the fertilized egg envelopes in golden severum, convic cichlid and discus, Cichlidae, teleost. Korean J. Electron Microsc. 27, 417-432.
  9. Donovan M J and Hart N H (1986) Cortical granule exocytosis is coupled with membrane retrieval in the egg of Brachydanio. J. Exp. Zool. 237, 391-405. https://doi.org/10.1002/jez.1402370312
  10. Grierson J P and Neville A C (1981) Helicoidal architecture of fish eggshell. Tissue Cell 13, 819-830. https://doi.org/10.1016/S0040-8166(81)80016-X
  11. Hamazaki T S, Nagahama Y, Luchi I, and Yamagami K (1989) A glycoprotein from the liver constitutes the inner layer of the egg envelope (zona pellucida interna) of the fish, Oryzias latipes. Dev. Biol. 133, 101-110. https://doi.org/10.1016/0012-1606(89)90301-1
  12. Harvey B, Kelley R N, and Ashwood-Smith M J (1983) Permeability of intact and dechorionated zebrafish embryo to glycerol and dimethyl sulfoxide. Cryobiology 20, 432-439. https://doi.org/10.1016/0011-2240(83)90033-0
  13. Huh M K, Choi J S, Heo Y S, and Lee B K (2007) Genetic diversity and population structure of Pseudobagrus fulvidraco in the Nakdong river. J. Life Sci. 17, 882-888. https://doi.org/10.5352/JLS.2007.17.7.882
  14. Joo K B and Kim D H (2013) Comparative ultrastructures of the fertilized egg envelopes in Danio rerio and Danio rerio var. frankei, Cyprinidae, Teleostei. Appl. Microsc. 43, 14-20. https://doi.org/10.9729/AM.2013.43.1.14
  15. Kim D H, Deung Y K, Kim W J, Reu D S, and Kang S J (1999) Comparative ultrastructures of the fertilized egg envelopes from three-spot gourami, pearl gourami and marble gourami, Belontiidae, teleost. Korean J. Microsc. 29, 343-351.
  16. Kim D H, Reu D S, and Deung Y K (1996) A comparative study on the ultrastructures of the egg envelope in fertilized eggs of fishes, Characidae, three species. Korean J. Microsc. 26, 277-291.
  17. Kim D H, Reu D S, and Deung Y K (1998) Comparative ultrastructures of the fertilized egg envelopes in three species, Cyprinidae, teleost. Korean J. Microsc. 28, 237-253.
  18. Kim I S and Park J Y (2002) Freshwater Fishes of Korea, pp. 235-236, (Kyo-Hak Publishing Co., Ltd., Seoul).
  19. Kwon J K, Jung H S, and Kim D H (2015) Comparative ultrastructures of the fertilized egg envelopes in Nothobranchius guentheri and Nothobranchius patrizii, Nothobranchiidae, Teleostei. Appl. Microsc. 45, 144-149. https://doi.org/10.9729/AM.2015.45.3.144
  20. Laale H W (1980) The perivitelline space and egg envelopes of bony fishes: a review. Copeia 2, 210-226.
  21. Lee Y H (1998) Ultrastructure of spermatozoa in the bagrid catfish, Pseudobagrus fulvidraco (Teleostei, Siluriformes, Bagridae). Korean J. Electron Microsc. 28, 39-48.
  22. Lim S G and Han C H (1997) Annual reproductive cycle of the banded catfish, Pseudobagrus fulvidraco (Richardson). J. Korean Fish. Soc. 30, 823-833.
  23. Lim S G and Han C H (2012) Effect of water temperatures and photoperiods on gonadal development in banded catfish Pseudobagrus fulvidraco. J. Fish. Mar Sci. Edu. 24, 854-861.
  24. Ohta T and Nashirozawa C (1996) Sperm penetration and transformation of sperm entry site in eggs of the freshwater teleost Rhodus ocellatus ocellatus. J. Morphol. 229, 191-200. https://doi.org/10.1002/(SICI)1097-4687(199608)229:2<191::AID-JMOR4>3.0.CO;2-4
  25. Oppen-Berntsen D O, Helvik J V, and Walther B T (1990) The major structural proteins of cod (Gadus morhua) eggshells and protein crosslinking during teleost egg hardening. Dev. Biol. 137, 258-265. https://doi.org/10.1016/0012-1606(90)90252-E
  26. Scapigliati G, Carcupino M, Taddei A R, and Mazzini M (1994) Characterization of the main egg envelope proteins of the sea bass Dicentrarchus labrax L. Mol. Reprod. Dev. 38, 48-53. https://doi.org/10.1002/mrd.1080380109
  27. Schmehl M K and Graham E F (1987) Comparative ultrastructure of the zona radiata from eggs of six species of salmonids. Cell Tissue Res. 250, 513-519.
  28. Stehr C M and Hawkes J W (1979) The comparative ultrastructure of the egg membrane and associated pore structures in the starry flounder, Platichthys stellatus (Pallas), and pink salmon, Oncorhynchus gorbuscha (Walbaum). Cell Tissue Res. 202, 347-356.