Transactions of the Korean Society of Mechanical Engineers A (대한기계학회논문집A)

The Korean Society of Mechanical Engineers (대한기계학회)
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Fabrication of BCP/Silica Scaffolds with Dual-Pore by Combining Fused Deposition Modeling and the Particle Leaching Method

압출 적층 조형법과 입자 추출법을 결합한 이중 공극 BCP/Silica 인공지지체의 제작

  • Received : 2016.04.22
  • Accepted : 2016.07.11
  • Published : 2016.10.01
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Abstract

In recent years, traditional scaffold fabrication techniques such as gas foaming, salt leaching, sponge replica, and freeze casting in tissue engineering have significantly limited sufficient mechanical property and cell interaction effect due to only random pores. Fused deposition modeling is the most apposite technology for fabricating the 3D scaffolds using the polymeric materials in tissue engineering application. In this study, 3D slurry mould was fabricated with a blended biphasic calcium phosphate (BCP)/Silica/Alginic acid sodium salt slurry in PCL mould and heated for two hours at $100^{\circ}C$ to harden the blended slurry. 3D dual-pore BCP/Silica scaffold, composed of macro pores interconnected with micro pores, was successfully fabricated by sintering at furnace of $1100^{\circ}C$. Surface morphology and 3D shape of dual-pore BCP/Silica scaffold from scanning electron microscopy were observed. Also, the mechanical properties of 3D BCP/Silica scaffold, according to blending ratio of alginic acid sodium salt, were evaluated through compression test.

조직 공학에서는 전통적인 인공지지체 제작 방식인 가스 발포, 염 침출, 스폰지 복제 그리고 동결주조 법 등이 이용되고 있다. 하지만 다양한 공극 형태 및 크기를 가지고 있어서 세포 상호 작용 효과 및 충분한 기계적 특성에 한계가 있다. 그러나 열 용해 적층 법은 조직공학에서 폴리머 재료를 이용하여 다양한 3차원 인공지지체를 제작할 수 있는 가장 적절한 기술이다. 따라서 본 연구에서는 PCL 몰드를 제작하고 실리카와 알긴산 나트륨 염을 포함하는 세라믹 슬러리를 제조하여 몰드에 주입시켰으며, 1일 동안 자연 건조를 시켰다. 제작된 3차원 슬러리 몰드는 PCL 몰드의 제거 및 슬러리를 경화시키기 위해 $100^{\circ}C$의 오븐에서 2시간 열처리 되었고, 열처리 후에 $1100^{\circ}C$에서 소결되었다. 제작된 인공지지체는 주사전자현미경을 통해 관찰되었고, 압축 시험을 통해 알긴산 나트륨 염의 혼합량에 따른 인공지지체의 기계적 특성은 평가되었다.

Keywords

References

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