International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Effect of β-carotene on Cell Growth Inhibition of KB Human Oral Cancer Cells

  • Yang, Sung-Su (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Kim, Su-Gwan (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Park, Byung-Sun (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Go, Dae-San (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Yu, Sun-Kyoung (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Kim, Chun Sung (Oral Biology Research Institute, School of Dentistry, Chosun University) ;
  • Kim, Jeongsun (Department of Dental Hygiene, Gwangju Health University) ;
  • Kim, Do Kyung (Oral Biology Research Institute, School of Dentistry, Chosun University)
  • Received : 2016.05.25
  • Accepted : 2016.07.05
  • Published : 2016.09.30
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Abstract

${\beta}-carotene$ is present in carrots, pumpkins, and sweet potatoes. It suppresses many types of cancers by regulating cellular proliferation and apoptosis through a variety of mechanisms. However, the effects of ${\beta}-carotene$ on oral cancer cells have not been clearly established. The main goal of this study was to investigate the effects of ${\beta}-carotene$ on cell growth and apoptosis in oral cancer cells. Our results demonstrate that treatment with ${\beta}-carotene$ induced inhibition of cell growth, and that the effect was dependent on ${\beta}-carotene$ treatment time and concentration in KB cells. Furthermore, treatment with ${\beta}-carotene$ induced nuclear condensation and fragmentation in KB cells. ${\beta}-carotene$ promoted proteolytic cleavage of procaspase-3, -7, -8 and -9 with associated increases in the concentration of cleaved caspase-3, -7, -8 and -9. In addition, the level of cleaved PARP was increased by ${\beta}-carotene$ treatment in KB cells. These results suggest that ${\beta}-carotene$ can suppress cell growth and induce apoptosis in KB human oral cancer cells, and that it may have potential usefulness in anti-cancer drug discovery efforts.

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References

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