Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex

  • Jang, Soo Hwa (School of Biological Sciences, University of Ulsan) ;
  • Kim, Ah-Ram (School of Biological Sciences, University of Ulsan) ;
  • Park, Neung-Hwa (Department of Internal Medicine, University of Ulsan College of Medicine, Ulsan University Hospital) ;
  • Park, Jeong Woo (School of Biological Sciences, University of Ulsan) ;
  • Han, In-Seob (School of Biological Sciences, University of Ulsan)
  • Received : 2016.06.14
  • Accepted : 2016.08.25
  • Published : 2016.09.30
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Abstract

Developmentally regulated GTP-binding protein 2 (DRG2) plays an important role in cell growth. Here we explored the linkage between DRG2 and G2/M phase checkpoint function in cell cycle progression. We observed that knockdown of DRG2 in HeLa cells affected growth in a wound-healing assay, and tumorigenicity in nude mice xenografts. Flow cytometry assays and [$^3H$] incorporation assays indicated that G2/M phase arrest was responsible for the decreased proliferation of these cells. Knockdown of DRG2 elicited down-regulation of the major mitotic promoting factor, the cyclin B1/Cdk1 complex, but upregulation of the cell cycle arresting proteins, Wee1, Myt1, and p21. These findings identify a novel role of DRG2 in G2/M progression.

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References

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