Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant activities and inhibitory effects on oxidative DNA damage of leaf from Zelkova serrata with ethyl acetate fractions and hot water extracts

느티나무 잎 에틸아세테이트 분획물 및 열수 추출물의 항산화 및 산화적 DNA 손상 억제 활성

  • Jang, Tae-Won (Department of Medicinal Plant Science, Jungwon University) ;
  • Park, Jae-Ho (Department of Medicinal Plant Science, Jungwon University)
  • Received : 2016.05.13
  • Accepted : 2016.06.15
  • Published : 2016.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Reactive oxygen species (ROS) has been played a critical role in damage of DNA. Recently, many effort is focusing to develop the natural antioxidants for controlling ROS. Zelkova serrata, Ulmaceae, is close as plants which are planted in front of Korea villages. Although Zelkova serrata is familiar with Koreans, those of antioxidant activities and protective effects on oxidative DNA damage haven't studied. We demonstrated antioxidant activities and inhibitory effects on oxidative DNA damage of Leaf from Zelkova serrata with ethyl acetate fractions (EA) and hot water extracts (HW). Between the extracts, EA showed higher activities in 1,1-diphenyl-2-picryl-hydrazyl, 2,2'-azino-bis[3-ethylbenzthiazoline-6-sulphonic acid radical scavenging, $Fe^{2+}$ chelating and reducing power than HW. Also, those of total phenolic content are 56.63 and 51.61 mg/g respectively. In addition, ${\phi}X$-174 RF I plasmid DNA cleavage assay for inhibitory effect by oxidative DNA damage was both EA and HW has significant protective effect on oxidative DNA damage. The results suggested that leaf from Zelkova serrata with ethyl acetate fractions and hot water extracts have surpassing potential as natural resources with antioxidant and inhibitory effect on oxidative DNA damage.

활성산소종은 DNA의 손상에서 중요한 역할을 한다. 최근 활성산소를 제어하고 조절하기 위해 천연항산화제를 개발하기 위해 많은 노력이 이루어지고 있다. 느티나무(Zelkova serrate)는 느릅나무과의 식물로 한국 마을 입구에 흔히 심어져 친숙한 식물이다. 하지만 느티나무의 항산화 활성 및 산화적 DNA 손상에 대한 방어효과에 대한 연구는 미흡한 실정이다. 본 연구에서 느티나무 잎의 에틸아세테이트 분획물 및 열수 추출물의 항산화 활성 및 산화적 DNA 손상에 대한 억제활성을 확인하였다. 에틸아세테이트 분획물은 열수 추출물에 비해 DPPH 라디칼 소거활성, ABTS 라디칼 소거활성, $Fe^{2+}$ 킬레이팅 활성 그리고 reducing power에서 높은 항산화 활성을 보였다. 또한, 페놀류 화합물 함량은 각각 에틸아세테이트 분획물은 56.63 mg/g 그리고 열수 추출물은 51.61 mg/g으로 분석됐다. ${\phi}X$-174 RF I plasmid DNA를 이용한 산화적 DNA 손상억제활성은 에틸아세테이트 분획물과 열수 추출물 모두 상당한 방어효과를 나타냈다. 따라서 느티나무 잎의 에틸아세테이트 분획물 및 열수 추출물은 뛰어난 항산화 활성 및 산화적 DNA 손상 억제 효과를 통한 천연 자원으로서의 잠재성을 보였다.

Keywords

References

  1. AOAC (1995) Official Methods of Analysis. Association of Official Analytical Chemist, Washington DC
  2. Arora A, Nair MG, Strasburg GM (1998) Structure activity relationships for antioxidant activities of a series of flavonoids in a liposomal system. J Free Radic Biol Med 24: 1355-1363 https://doi.org/10.1016/S0891-5849(97)00458-9
  3. Barman Balfour JA, Foster RH (1999) Deferiprone: a review of its clinical potential in iron overload in betathalassaemia major and other transfusion-dependent diseases. Drugs 58: 553-578 https://doi.org/10.2165/00003495-199958030-00021
  4. Benz EJ (2001) Hemoglobinopathies. In: Braunwald E, Fauci AS, Kasper DL, Hauser SL, Longo DL, Jameson JL (eds) Harrison's Principles of Internal Medicine, 15th edn. New York
  5. Bondet V, Brand Williams W, Berset C (1997) Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. Lebensm Wiss U-Technol 30: 609-615 https://doi.org/10.1006/fstl.1997.0240
  6. Cho IY, Sheen YY (2009) Effect of dioxin on the change of mitochondrial inner membrane potential and the induction of ROS. J Environ Toxicol 24: 33-41 https://doi.org/10.1002/tox.20391
  7. Choe SY, Yang KH (1982) Toxicological studies of antioxidants butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA). Korean J Food Sci Technol 14: 283-288
  8. Halliwell B (1995) Antioxidant characterization: methodology and mechanism. Biochem Pharmacol 49: 1341-1348 https://doi.org/10.1016/0006-2952(95)00088-H
  9. Harold ES, Darrell EA, Evan IF, John AM (2007) A review of the interaction among dietary antioxidants and reactive oxygen species. J Nutr Biochem 18: 567-579 https://doi.org/10.1016/j.jnutbio.2006.10.007
  10. Hassas-Roudsari M, Chang PR, Pegg RB, Tyler RT (2009) Antioxidant capacity of bioactives extracted from canola meal by subcritical water, ethanol and hot water extraction. J Food Chem 114: 717-726 https://doi.org/10.1016/j.foodchem.2008.09.097
  11. Hayashi Y, Ohara S, Takahashi T (1987) Isolation of new 3-hydroxylated flavonoid C-glucosides from the heartwood of Zelkova serrata Makino and other Zelkova species. Mokuzai Gakkaishi 33: 511-520
  12. Hsu B, Coupar IM, Ng K (2006) Antioxidant activity of hot water extract from the fruit of the Doum palm, Hyphaene thebaica. J Food Chem 98: 317-328 https://doi.org/10.1016/j.foodchem.2005.05.077
  13. Hwang JY, Lee HS, Han JS (2011) Protective effect of Sasa borealis leaf extract on AAPH-induced oxidative stress in LLC-PK1 cells. J Food Sci Nutr 16: 12-17
  14. Johnson TM, Yu ZX, Ferrans VJ, Lowenstein RA, Finkel T (1996) Reactive oxygen species are downstream mediators of p53-dependent apoptosis. Proc Natl Acad Sci 93: 11848-11852 https://doi.org/10.1073/pnas.93.21.11848
  15. Jung Y, Surh Y (2001) Oxidative DNA damage and cytotoxicity unduced by copper-stimulated redox cycling of salsolinol. a neurotoxic tetrahydroisoquinoline alkalooid. Free Radic Biol Med 30: 1407-1417 https://doi.org/10.1016/S0891-5849(01)00548-2
  16. Kim JH, Lee HJ, Kim GS, Choi DH, Lee SS, Kang JK, Chae C, Paik NW, Cho MH (2004) Inhibitory effects of 7-hydroxy-3-methoxy-cadalene on 4-(methylinitrosamino)-1-(3-pyridyl)-1-butanone(NNK)-induced lung tumorigenesis in A/J mice. Cancer Lett 213: 139-145 https://doi.org/10.1016/j.canlet.2004.03.049
  17. Lee CB (2003) The illustrated guide to Korean flora (I). Hangmunsa, Seoul
  18. Lee EK, Kwon WY, Lee JW, Yoon JA, Chung KH, Song BC, An JH (2014) Quality characteristics and antioxidant activity of vinegar supplemented added with Akebia quinata fruit during fermentation. J Korean Soc Food Sci Nutr 43: 1217-1227
  19. Lee JC, Kim HR, Kim J, Jang YS (2002) Antioxidant Property of an Ethanol Extract of the Stem of Opuntia ficus-indica var. Saboten. J Agric Food Chem 50: 6490-6496 https://doi.org/10.1021/jf020388c
  20. Lee JH, Lee BG, Park AR, Lee KJ, Choi DW, Han SH, Choi GP, Kim JD, Kim JC, Ahn JH, Lee HY, Shin IC, Park HJ (2011) In vitro antioxidant potential and oxidative DNA damage protecting activity of the ethanol extracts of Cacalia firma Komar. Appl Biol Chem 54: 258-264 https://doi.org/10.3839/jabc.2011.042
  21. Lee SH, Choi DH, Lee HJ, Kang HY (2000) Studies on biological activity of wood extractives (II)-antimicrobial and antioxidative compound isolated from heartwood of Zelkova serrata. J Korean Wood Sci Technol 28: 32-41
  22. Lee YS, Joo EJ, Kim NW (2006) Polyphenol contents and antioxidant activity of Lepistanuda. J Korean Soc Food Sci Nutr 35: 1309-1314 https://doi.org/10.3746/jkfn.2006.35.10.1309
  23. Maxwell SJ (1995) Prospects for the use of antioxidant therapies. Drugs 49: 345-361 https://doi.org/10.2165/00003495-199549030-00003
  24. Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, van Leeuwen PA (2001) Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr 74: 418-425 https://doi.org/10.1093/ajcn/74.4.418
  25. Oliver NF, Brittenham GM (1997) Iron-chelating therapy and the treatment of thalassemia. Blood 89: 739-761
  26. Oliveri NF, Nathan DG, MacMilan JH, Wayne AS, Liu PP, McGee A (1994) Survival in medically treated patients with homozygous betathalassemia. N Engl J Med 331: 574-578 https://doi.org/10.1056/NEJM199409013310903
  27. Oyaizu M (1986) Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J Nutr 44: 307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  28. Park YK, Choi SH, Kim SH, Han JG, Chung HG (2007) Changes in antioxidant activity, total phenolics and vitamin C content during fruit ripening in Rubus occidentalis. Korean J Plant Res 20: 461-465
  29. Prakash CP, Garima U, Brahma NS, Harikesh B (2007) Antioxidant and free radical-scavenging activities of seeds and agri-wastes of some varieties of soybean (Glycine max). Food Chem 104: 783-790 https://doi.org/10.1016/j.foodchem.2006.12.029
  30. Que F, Mao L, Pan X (2006) Antioxidant activities of five Chinese rice wines and the involvement of phenolic compounds. Food Research International 39: 581-587 https://doi.org/10.1016/j.foodres.2005.12.001
  31. Rice-Evans CA, Sampson J, Bramley PM, Holloway DE (1997) Why do we expect carotenoids to be antioxidants in vivo ? J Free Radic Res 26: 381-398 https://doi.org/10.3109/10715769709097818
  32. Sakanaka S, Tachibana Y, Okada Y (2005) Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha). J Food Chem 89: 569-575 https://doi.org/10.1016/j.foodchem.2004.03.013
  33. Sonakul D, Thakerngpol K, Pacharee P (1988) Cardiac pathology in 76 thalassemia patients. Birth Defects Orig Article Ser 23: 177-191
  34. Stohs SJ, Bagchi D (1995) Oxidative mechanism in the toxicity of metal ions. Free Radic Biol Med 18: 321-336 https://doi.org/10.1016/0891-5849(94)00159-H
  35. Van den Berg R, Haenen GR, Van den Berg H, Bast A (1999) Applicability of an improved trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. J Food Chem 66: 511-517 https://doi.org/10.1016/S0308-8146(99)00089-8
  36. Warnholtz A, Münzel T (2000) Why do antioxidants fail to provide clinical benefit? Curr Control Trials Cardiovasc Med 1: 38-40 https://doi.org/10.1186/CVM-1-1-038
  37. Wei QY, Zhou B, Jun Y, Liu ZL (2006) Synergistic effect of green tea polyphenols with trolox on free radical-ed oxidative DNA damage. Food Chem 96: 90-95 https://doi.org/10.1016/j.foodchem.2005.01.053
  38. Yeo JS, Kim JS, Kim MO, Jeong HJ, Yu CY, Park DS, Kim MJ (2010) Comparative study of electron donating ability, reducing power, antimicrobial activity and inhibition of ${\alpha}$-glucosidase by sorghum bicolor extracts. Korean J Food Sci Technol 42: 598-604
  39. Yoshino M, Murakami K (1998) Interaction of iron with polyphenolic compounds: application to antioxidant characterization. Anal Biochem 257: 40-44 https://doi.org/10.1006/abio.1997.2522
  40. Zurlo MG, De Stefano P, Borgna-Pignatti A, Di Palma A, Piga A, Melevendi C (1989) Survival and causes of death in thalassaemia major. Lancet 2: 27-30