해양환경안전학회지 (Journal of the Korean Society of Marine Environment & Safety)

  • 제22권5호
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  • Pages.500-507
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  • 2016
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  • 1229-3431(pISSN)
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  • 2287-3341(eISSN)
해양환경안전학회 (The Korean Society of Marine Environment and safety)
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저온 배양한 L-type 로티퍼(Brachionus plicatilis)의 적정 영양강화 수온, 시간 및 영양강화제 종류

Optimal Enrichment Temperature, Time and Materials for L-type Rotifer (Brachionus plicatilis) Cultured at a Low Temperature

  • 유해균 (동해수산연구소 양식산업과) ;
  • 변순규 (동해수산연구소 양식산업과) ;
  • 최진 (동해수산연구소 양식산업과) ;
  • 남명모 (동해수산연구소 양식산업과) ;
  • 이해영 (동해수산연구소 양식산업과) ;
  • 강희웅 (동해수산연구소 양식산업과) ;
  • 이주 (동해수산연구소 양식산업과)
  • Yoo, Hae-Kyun (Aquaculture Industry Research Division, East Sea Fisheries Research Institute) ;
  • Byun, Soon-Gyu (Aquaculture Industry Research Division, East Sea Fisheries Research Institute) ;
  • Choi, Jin (Aquaculture Industry Research Division, East Sea Fisheries Research Institute) ;
  • Nam, Myeong-Mo (Aquaculture Industry Research Division, East Sea Fisheries Research Institute) ;
  • Moon Lee, Haeyoung (Aquaculture Industry Research Division, East Sea Fisheries Research Institute) ;
  • Kang, Hee Wong (Aquaculture Industry Research Division, East Sea Fisheries Research Institute) ;
  • Lee, Chu (Aquaculture Industry Research Division, East Sea Fisheries Research Institute)
  • 투고 : 2016.07.29
  • 심사 : 2016.08.29
  • 발행 : 2016.08.31
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초록

본 연구는 유용 냉수성 어류 등의 종묘생산 시 초기의 성장과 생존률을 향상시키기 위하여, 저온에서 증식할 수 있는 저온 내성을 가진 로티퍼(Brachionus plicatilis)를 배양하여, 수온 및 시간에 따른 영양강화 실험을 실시하였다. 로티퍼의 저온 배양은 $20^{\circ}C$에서 배양하던 로티퍼의 수온을 점차적으로 낮추면서 활력이 있는 개체의 선별 배양을 반복하여 최종적으로 $10^{\circ}C$에서 사육하였다. 영양강화는 상업적으로 이용되는 영양강화제인 A, S, SCV 및 SCP의 4종류를 사용하여 10, 15 및 $20^{\circ}C$의 수온에서 6, 12 및 24시간 실시하였다. 수온 $10^{\circ}C$에서 50일간 로티퍼의 증식률 실험을 한 결과 접종 밀도 $350{\pm}7.9$개체/ml에서 최종 배양 밀도는 $1,064{\pm}5.7$개체/ml로 약 3배 개체수가 증가하였다. 영양강화제에 포함된 지방산을 분석한 결과, n3계 고도불포화 지방산인 eicosapentaenoic acid (EPA, C20:5n-3) 및 docosahexaenoic acid(DHA, C22:6n-3)는 SCP에서 각각 15.49%, 35.03 %로 높게 나타났다. 영양강화한 로티퍼의 지방산 조성은 영양강화제에 따라 영향을 받았다. EPA는 SCP가 영양강화 수온 및 시간에 관계없이 2 % 이상을 차지하여 다른 영양강화제보다 높은 비율을 나타냈다. DHA는 S가 $15^{\circ}C$, 24시간 실험구에서 12.40 %로 높게 나타났다. 영양강화 로티퍼의 EPA와 DHA의 비율을 고려하면 S를 $20^{\circ}C$에서 12시간 영양강화한 실험구가 각각 3.09, 11.65 %로 높게 나타났다.

This study was undertaken to improve the survival and early life growth rates of cold-water fish by culturing rotifer (Brachionus plicatilis) with low-temperature tolerance. The enrichment experiment was carried out at different temperatures and over different time intervals. Cultivation of the rotifer at low temperatures was repeated, with the selected and cultured as the water temperature was gradually lowered from $20^{\circ}C$ to $10^{\circ}C$. Enrichment of the rotifer was completed using A, S, SCV and SCP. Enrichment was carried out after 6, 12 and 24 hours at three different temperatures (10, 15 and $20^{\circ}C$). In the growth experiments, the rotifer increased to approximately triple their original size, from $350{\pm}7.9ind./ml$ to $1,064{\pm}5.7ind./ml$ at $10^{\circ}C$ over 50 days. The fatty acid composition of the four enrichment materials was species-specific, with the highest ratios belonging to eicosapentaenoic acid (EPA, C20:5n-3) and docosahezaenoic acid (DHA, C22:6n-3) in SCP. The fatty acid composition of the rotifers was affected by the enrichment materials. The EPA (% of total fatty acid) was more than 2 % in SCP, which showed a higher ratio than the other enrichment materials. DHA was higher in S reaching 12.40 % at $15^{\circ}C$ for 24 hours. The highest levels of EPA (3.09 %) and DHA (11.65 %) were obtained after the rotifers were enriched with S at $20^{\circ}C$ for 12hours.

키워드

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피인용 문헌

  1. 다양한 Rotifer (Brachionus plicatilis) 영양강화가 태평양 대구(Gadus macrocephalus) 자어의 성장 및 지방산 조성에 미치는 영향 vol.53, pp.4, 2016, https://doi.org/10.5657/kfas.2020.0530
  2. Effects of light intensity and photoperiod on survival and growth of walleye pollock (Gadus chalcogrammus) larvae vol.33, pp.4, 2016, https://doi.org/10.13000/jfmse.2021.8.33.4.859