Advances in nano research

  • 제4권3호
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  • Pages.167-179
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  • 2016
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  • 2287-237X(pISSN)
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  • 2287-2388(eISSN)
테크노프레스 (Techno-Press)
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DOI QR코드

DOI QR Code

The acute and sub-acute toxicity of C60/PVP complex in vivo

  • Dumpis, Marina A. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Iljin, Viktor V. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Litasova, Elena V. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Nikolaev, Dmitry N. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Bulion, Valentina V. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Krylova, Irina B. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Okunevich, Irina V. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Rodionova, Olga M. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Safonova, Albina F. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Selina, Elena N. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Piotrovsky, Levon B. (Department of Neuropharmacology, Institute of Experimental Medicine)
  • 투고 : 2016.02.15
  • 심사 : 2016.06.24
  • 발행 : 2016.09.25
⟨ 이전 논문 다음 논문 ⟩

초록

The detailed study of acute and sub-acute toxicity of the complex polyvinylpyrrolidon (PVP 20 kDa)-wrapped fullerene $C_{60}$ after intraperitoneal (ip) administration was carried out on adult male Wistar rats. The $LD_{50}$ value of $C_{60}/PVP$ complex was found to be 7, 8 g/kg. In sub-acute study which lasted for 30 days the rats were exposed to daily administration of the complex in the doses of 350 or 700 mg/kg. All animals survived during the study and had no significant changes in clinical signs, organ weight, hematological and biochemical parameters of blood. The electrophysiological properties of myocardium and the excretory function of kidneys remained normal. Histological analysis of liver, kidney and spleen at the end of the study also did not demonstrate toxic alterations. It was thus established that intraperitoneal administration of complex $C_{60}/PVP$ has no toxic effect. These results suggest that $C_{60}/PVP$ has no acute and sub-acute toxicity and is a perspective substance for potential application in biology and medicine.

키워드

참고문헌

  1. Andrievsky, G.V., Kosevich, M.V., Vovk, O.M., Shelkovsky, S. and Vashchenko, L.A. (1995), "On the production of an aqueous colloidal solution of fullerene," J. Chem. Soc., Chem. Commun., 1281-1282.
  2. Anilkumar, P., Lu, F., Cao, L., Luo, P.G., Liu, J.H., Sahu, S., Tackett. K.N., Wang. Y. and Sun, Y.P. (2011), "Fullerenes for applications in biology and medicine," Curr. Med. Chem., 18(14), 2045-59. https://doi.org/10.2174/092986711795656225
  3. Anish, G., Jack, B.H., John, B. and Vander, S. (2004), "Size analysis of single fullerene molecules by electron microscopy," Carbon, 42, 1907-1915. https://doi.org/10.1016/j.carbon.2004.03.022
  4. Aoshima, H., Saitoh, Y., Ito, S., Yaman, S. and Miwa, N. (2009), "Safety evaluation of highly purified fullerenes (HPFs): based on screening of eye and skin damage," J. Toxicol. Sci., 4, 555-562.
  5. Aoshima, H., Yamana, S., Nakamura, S. and Mashino, T. (2010), "Biological safety of water-soluble fullerenes evaluated using tests for genotoxicity, phototoxicity, and pro-oxidant activity," J. Toxicol. Sci., 35(3), 401-409. https://doi.org/10.2131/jts.35.401
  6. Aschberger, K., Johnston, H.J., Stone, V., Aitken, R.J., Tran, C. L., Hankin, S.M., Peters, S.A.K. and Christensen, F.M. (2010), "Review of fullerene toxicity and exposure-Appraisal of a human health risk assessment, based on open literature," Regulatory Toxicology and Pharmacology, 58, 455-473. https://doi.org/10.1016/j.yrtph.2010.08.017
  7. Baati, T., Bourasset, F., Gharbi, N., Njim, L., Abderrabba, M., Kerkeni, A., Szwarc, H. and Moussa, F. (2012), "The prolongation of the lifespan of rats by repeated oral administration of [60]fullerene," Biomaterials, 33, 4936-4946. https://doi.org/10.1016/j.biomaterials.2012.03.036
  8. Bakry, R., Vallant, R.M., Najam-ul-Haq, M., Rainer, M., Szabo, Z., Huck, C.W. and Bonn, G.K. (2007), "Medicinal applications of fullerenes," Int. J. Nanomedicine, 2(4), 639-649.
  9. Bang, J.J., Guerrero, P.A., Lopez, D.A., Murr, L.E. and Esquivel, E.V. (2004), "Carbon nanotubes and other fullerene nanocrystals in domestic propane and natural gas combustion streams," J. Nanosci. Nanotechnol., 4, 716-718. https://doi.org/10.1166/jnn.2004.095
  10. Bensasson, R.V., Bienvenue, E., Dellinger, M., Leach, S. and Seta, P. (1994), "C60 in Model Biological Systems. A Visible-UV Absorption Study of Solvent-Dependent Parameters and Solute Aggregation," J. Phys. Chem., 98, 3492-3500. https://doi.org/10.1021/j100064a035
  11. Brant, J., Labille, J, Bottero, J.Y. and Wiesner, M.R. (2006), "Characterizing the impact of preparation method on fullerene cluster structure and chemistry," Langmuir, 22, 3878-3885. https://doi.org/10.1021/la053293o
  12. Dugan, L.L., Lovett, E.G., Quick, K.L., Lotharius, J., Lin, T.T. and O'Malley, K.L. (2001), "Fullerene-based antioxidants and neurodegenerative disorders," Parkinsonism Relat. Disord., 7(3), 243-246. https://doi.org/10.1016/S1353-8020(00)00064-X
  13. Dugan, L.L., Turetsky, D.M., Du, C., Lobner, D., Wheeler, M., Almli, C.R., Shen, C.K.F., Luh, T.Y., Choi, D.W. and Lin, T.S. (1997), "Carboxyfullerenes as neuroprotective agents," PNAS, 94, 9434-9439. https://doi.org/10.1073/pnas.94.17.9434
  14. Dugan, L.L., Turetsky, D.M., Du, C., Lobner, D., Wheeler, M., Almli, C.R., Shen, C.K.F., Luh, T.Y., Choi, D.W., Lin, T.S., McEwen, C.N., McKay, R.G. and Larsen, B.S. (1992), "C60 as a Radical Scavenger," J. Am. Chem. Soc., 114, 4412-4414. https://doi.org/10.1021/ja00037a064
  15. Gharbi, N., Pressac, M., Hadchouel, M., Szwarc, H., Wilson, S.R. and Moussa, F. (2005), "Fullerene is an in vivo powerful antioxidant with no acute or sub-acute toxicity," Nano Letters, 5, 2578-2585. https://doi.org/10.1021/nl051866b
  16. Guldi, D.M., Rahman, G.M.A., Sgobba, V. and Ehli, C. (2006), "Multifunctional molecular carbon materialsfrom fullerens to carbon nanotubes," Chem.Soc Rev., 35, 471-487. https://doi.org/10.1039/b511541h
  17. Hu, Z., Zhang, C., Huang, Y., Sun, S., Guan, W. and Yao, Y. (2012), "Phgotodynamic anticancer activities of water-soluble $C_{60}$ derivatives and their diological consequences in a Hela cell line," Chemico-Biological Interactuons, 195, 86-94. https://doi.org/10.1016/j.cbi.2011.11.003
  18. Inui, S., Aoshima, H., Nishiyama, A. and Itami, S. (2011), "Improvement of acne vul garis by topical fullerene application: unique impact on skin care," Nanomedicine: Nanotechnology, Biology, and Medicine, 7, 238-241. https://doi.org/10.1016/j.nano.2010.09.005
  19. Jafvert, C.T., Kulkarni, P.P. (2008), "Buckminsterfullerene‟s (C60) octanol-water partition coefficient (K ow) and aqueous solubility," Environ. Sci. Technol., 42(16), 5945-5950. https://doi.org/10.1021/es702809a
  20. Johnston, H.J., Hutchison, G.R., Christensen, F.M., Aschberger, K. and Stone, V. (2010), "The biological mechanisms and physicochemical characteristics responsible for driving fullerene toxicity," J. Toxicol. Sci., 114(2), 162-182. https://doi.org/10.1093/toxsci/kfp265
  21. Kato, S., Aoshima, H., Saitoh, Y. and Miwa, N. (2009), "Biological safety of lipofullerene composed of squalane and fullerene-C60 upon mutagenesis, photocytotoxicity, and permeability into the human skin tissue," Basic Clin. Pharmacol. Toxicol., 104, 483-487. https://doi.org/10.1111/j.1742-7843.2009.00396.x
  22. Kato, S., Aoshima, H., Saitoh, Y. and Miwa, N. (2010), "Fullerene-$C_{60}$/liposome complex: Defensive effects against UVA-induced damages in skin structure, nucleus and collagen type I/IV fibrils, and the permeability into human skin tissue," J. Photochem. Photobiol. B: Biology, 98, 99-105. https://doi.org/10.1016/j.jphotobiol.2009.11.010
  23. Kato, S., Aoshima, H., Saitoh, Y. and Miwa, N. (2014), "Fullerene-C60 derivatives 14prevent UV-irradiation/TiO2-induced cytotoxicity on keratinocytes and 3D-skin tissues through antioxidant actions," J. Nanosci. Nanotechnol., 14(5), 3285-91. https://doi.org/10.1166/jnn.2014.8719
  24. Kiselev, O., Kozeletskaya, K., Melenevskaya, E., Vinogradova, L., Kever, E., Klenin, S., Zgonnik, V., Dumpis, M. and Piotrovsky L.B. (1998), "Antiviral activity of fullerene C60 with poly(N-vinylpyrrolidone) complex," Mol. Mat., 11, 121-124.
  25. Kolosnjaj, J., Szwarc, H. and Moussa, F. (2007), "Toxicity studies of fullerenes and derivatives," Adv. Exp. Med. Biol., 620,168-180. https://doi.org/10.1007/978-0-387-76713-0_13
  26. Krakovjak, M.G., Anufrieva, E.V., Anan„eva, T.D. and Nekrasova, T.N. (2006), "Water-soluble complexes of poly(N-vinylamides) of various structures with C60 and C70 fullerenes," Polym. Sci. Ser. A, 48, (6), 590-595. https://doi.org/10.1134/S0965545X06060058
  27. Krakovjak, M.G., Anufrieva, E.V., Anan„eva, T.D., Piotrovskij, L.B. and Nekrasova, T.N. "Water-soluble complex of fullerene with poly-n-vinylpyrrolidone and method for preparing these complexes," 10.07.2005 Patent RU 2255942.
  28. Krustic, P.J., Wassermann, E., Keizer, P.N., Morton, J.R. and Preston, K.F. (1991), "Radical reaction of C60," Science, 254, 1183-1185. https://doi.org/10.1126/science.254.5035.1183
  29. Lens, M. (2009), "Use of fullerenes in cosmetics," Recent patents on Biotechnol., 3, 118-123. https://doi.org/10.2174/187220809788700166
  30. Liu, Q., Cui, Q., Li, X.J. and Jin, L. (2014), "The applications of buckminsterfullerene $C_{60}$ and derivatives in orthopedic research," Connect. Tissue Res., 55(2), 71-9. https://doi.org/10.3109/03008207.2013.877894
  31. Long, Y.Ch., Lub, F.J. and Linc, J.T. (1995), "Free radical scavenging activity of water-soluble fullerenols," J. Chem. Soc., Chem. Commun., 1283.
  32. Mori, T., Takada, H., Ito, S., Matsubayashi, K., Miwa, N. and Sawaguchi T. (2006), "Preclinical studies on safety of fullerene upon acute oral administration and evaluation for no mutagenesis," Toxicology, 225, 48-54. https://doi.org/10.1016/j.tox.2006.05.001
  33. Moussa, F., Trivin, F., Ceolin, R., Hadchouel, V., Sizaret, P.Y., Greugny, V., Rassat, A., Szwarc, H. (1996), "Early effects of $C_{60}$ administration in Swiss mice: a preliminary account for in vivo $C_{60}$ toxicity," Ful. Sci. Technology, 4(1), 21-29. https://doi.org/10.1080/10641229608001534
  34. Murayama, H., Tomonoh, S., Alford, J.M. and Karpuk, M.E. (2005), "Fullerene Production in Tons and More: From Science to Industry," Fuller Nanotub Car N, 12 (1), 1-9. https://doi.org/10.1081/FST-120027125
  35. Murr, L.E. and Soto, K.F. (2005), "A TEM study of soot, carbon nanotubes, and related fullerene nanopolyhedra in common fuel-gas combustion sources," Mater.Characteriz., 55, 50-65. https://doi.org/10.1016/j.matchar.2005.02.008
  36. Nielsen, G.D., Roursgaard, M., Jensen, K.A., Poulsen, S.S. and Larsen, S.T. (2008), "In vivo biology and toxicology of fullerenes and their derivatives," Basic. Clin. Pharmacol. Toxicol., 103, 197-208. https://doi.org/10.1111/j.1742-7843.2008.00266.x
  37. Park, E., Roh, J., Kim, Y. and Park, K. (2010), "Induction of inflammatory responses by carbon fullerene (C60) in cultured RAW264.7 cells and intraperitoneally injected mice," Toxicol. Res., 26(4), 267-273. https://doi.org/10.5487/TR.2010.26.4.267
  38. Piotrovsky, L.B. (2006), "Biological activity of pristine fullerene C60," Carbon Nanotechnology, Ed. L. Dai. Elsevier, Dayton. USA.
  39. Piotrovsky, L.B., Dumpis, M.A., Litasova, E.V., Eropkin, M.Y., Eropkina, E.M. and Kiselev, O.I. (2011), "Dependence of biological effects of fullerene C60 in vitro from the type of preparations," Fuller Nanotub Car N, 19(1), 147-153.
  40. Piotrovsky, L.B. and Kiselev, O.I. (2004), "Fullerenes and viruses," Fuller Nanotub Car N, 12(1), 397-403.
  41. Piotrovsky, L.B., Dumpis, M.A., Poznyakova, L.N. and Kiselev, O.I. (2005), "Influence of complexes fullerene $C_{60}$ with ${\gamma}$-CD on the morphology influenza viruses," Questions of Biological, Medicinal and Pharmaceutical Chemistry, 3, 21-24.
  42. Piotrovsky, L.B., Eropkin, M.Y., Eropkina, E.M., Dumpis, M.A. and Kiselev, O.I. (2008), "Biological effects in cell cultures of fullerene C60: dependence on aggregation state," Medicinal Chemistry and Pharmacological Potential of Fullerenes and Carbon Nanotubes, Cataldo F., Da Ros T., Springer.
  43. Piotrovsky, L.B. (2013), Essays on Nanomedicine, Europe House, Saint Petersburg, Russia.
  44. Podolski, I.Y., Kondratjeva, E.V., Shcheglov, I.V., Dumpis, M.A. and Piotrovsky, L.B. (2002), "Fullerene C60 complexed with poly (N-vinylpyrrolidone) prevents the disturbance of long-term memory consolidation," Phys. Solid States, 44, 552-554.
  45. Podolski, I.Y., Kondratjeva, E.V., Gurin, S.S., Dumpis, M.A. and Piotrovsky, L.B. (2004), "Fullerene C60 complexed with poly(N-vinylpyrrolidone) prevents the disturbance of long-term memory consolidation induced by cycloheximide," Fuller Nanotub Car N, 12, 421-424.
  46. Popov, V.A., Tyunin, M.A., Zaitseva, O.B., Karaev, R.H., Sirotinkin, N.V., Dumpis, M.A. and Piotrovsky, L.B. (2008), "C60/PVP complex-no toxicity after introperitoneal injection to rats," Fuller Nanotub Car N, 16(5), 693-697. https://doi.org/10.1080/15363830802317130
  47. Prozovskii, V.B. and Prozorovskaia. M.P. (1980), "Table method of determining the ED50 (LD50) of substances with low biological activity". Farmacol. Toksikol., 43(6), 733-735.
  48. Schavlovsky M.M., Soloviev K.S., Dumpis M.A., Litasova E.V. and Piotrovsky L.B. (2008), "Complexes of fullerene C60 with blood plasma proteins," Nanotechnology in Biology and Medicine, Ed. E.V. Schlyachto, SPb, Russia.
  49. Sushko, M.L., Klenin, S.I., Piotrovsky, L.B. and Poznyakova, L.N. (1999), "Light scattering in water solution of fullerene-containing polymers. Part 2. Effect of the molecular weight of the scatter molecules," Tech. Phys. Lett., 25, 778-779. https://doi.org/10.1134/1.1262632
  50. Takada, H., Mimura, H., Xiao, L., Islam, R.M., Matsubayashi, K., Ito, S. and Miwa, N. (2006), "Innovative antioxidant: Fullerene (INCI #: 7587) is as radical sponge on the skin. Its high level of safety, stability and potential as premier anti-aging and whitening cosmetic ingredient," Fuller Nanotub Car N, 14(2-3), 335-341. https://doi.org/10.1080/15363830600665433
  51. Takahashi, M., Kato, N., Doi, Y., Hagiwara, A., Hiirata-Koizumi, M., Ono, A., Kubota, R., Nishimura, T. and Hirose, A. (2012), "Sub-acute toxicity study with fullerene C60 in rats," J. Toxicol. Sci., 37(2), 353-361. https://doi.org/10.2131/jts.37.353
  52. Tsuchiya, T., Oguri, I., Yamakoshi, Y.N. and Miyata, N. (1996), "Effect of [60]fullerene on the chondrogenesis in mouse embryonic limb bulb cell culture system," Full. Sci. Technol., 4, 989-999. https://doi.org/10.1080/10641229608001157
  53. Tsuchiya, T., Oguri, I., Yamakoshi, Y.N. and Miyata, N. (1996) "Novel harmful effects of fullerene on mouse embryos in vivtro and in vivo," FEBS Lett., 393, 139-145. https://doi.org/10.1016/0014-5793(96)00812-5
  54. Xiao, Y. and Wiesner, M.R. (2012), "Characterization of surface hydrophobicity of engineered nanoparticles," J. Hazard. Mater., 215-216, 146-151. https://doi.org/10.1016/j.jhazmat.2012.02.043
  55. Yamakoshi, Y.N., Yagami, T., Fukuhara, K., Fukuhara, K., Sueyoshi, S. and Miyata, N. (1994), "Solubilization of fullerenes into water with polyvinylpyrrolidone applicable to biological tests," J. Chem. Soc., Chem. Commun., 517-518.
  56. Yamashita, K., Yoshioka, Y., Pan. H., Taira, M., Ogura, T., Nagano, T., Aoyama, M., Nagano, K., Abe, Y., Kamada, H., Tsunoda, S.I., Aoshima, H., Nabeshi, H., Yoshikawa, T. and Tsutsumi, Y. (2013), "Biochemical and hematologic effects of polyvinylpyrrolidone-wrapped fullerene $C_{60}$ after oral administration," Pharmazie, 68(1), 54-7.
  57. Vengerovich, N.G., Tuynin, M.A., Antonenkova, E.V., Konshakov, Y.O., Bolehan, A.V., Zaytceva, O.B., Stukov, A.V., Boyarkin, M.N. and Popov, V.A. (2012), "Biological activity of fullerene $C_{60}$ nanobiocomposites," Immunology, 12, 161-177.
  58. Zhao, B., He, Y.Y., Chignell, C.F., Yin, J.J., Andley, U. and Roberts, J.E. (2009), "Difference in phototoxicity of cyclodextrin complexed fullerene [(${\gamma}-CyD)2/C_{60}$] and its aggregated derivatives toward human lens epithelial cells," Chem. Res. Toxicol., 22(4), 660-66. https://doi.org/10.1021/tx800478u

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