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The acute and sub-acute toxicity of C60/PVP complex in vivo

  • Dumpis, Marina A. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Iljin, Viktor V. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Litasova, Elena V. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Nikolaev, Dmitry N. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Bulion, Valentina V. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Krylova, Irina B. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Okunevich, Irina V. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Rodionova, Olga M. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Safonova, Albina F. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Selina, Elena N. (Department of Neuropharmacology, Institute of Experimental Medicine) ;
  • Piotrovsky, Levon B. (Department of Neuropharmacology, Institute of Experimental Medicine)
  • Received : 2016.02.15
  • Accepted : 2016.06.24
  • Published : 2016.09.25
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Abstract

The detailed study of acute and sub-acute toxicity of the complex polyvinylpyrrolidon (PVP 20 kDa)-wrapped fullerene $C_{60}$ after intraperitoneal (ip) administration was carried out on adult male Wistar rats. The $LD_{50}$ value of $C_{60}/PVP$ complex was found to be 7, 8 g/kg. In sub-acute study which lasted for 30 days the rats were exposed to daily administration of the complex in the doses of 350 or 700 mg/kg. All animals survived during the study and had no significant changes in clinical signs, organ weight, hematological and biochemical parameters of blood. The electrophysiological properties of myocardium and the excretory function of kidneys remained normal. Histological analysis of liver, kidney and spleen at the end of the study also did not demonstrate toxic alterations. It was thus established that intraperitoneal administration of complex $C_{60}/PVP$ has no toxic effect. These results suggest that $C_{60}/PVP$ has no acute and sub-acute toxicity and is a perspective substance for potential application in biology and medicine.

Keywords

References

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