Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Isolation and Identification of Prepubertal Buffalo (Bubalus bubalis) Spermatogonial Stem Cells

  • Feng, Wanyou (State Key Laboratory of Conservation and Untilization of Subtropical Agro-Bioresources, Guangxi University) ;
  • Chen, Shibei (State Key Laboratory of Conservation and Untilization of Subtropical Agro-Bioresources, Guangxi University) ;
  • Do, Dagiang (State Key Laboratory of Conservation and Untilization of Subtropical Agro-Bioresources, Guangxi University) ;
  • Liu, Qinyou (State Key Laboratory of Conservation and Untilization of Subtropical Agro-Bioresources, Guangxi University) ;
  • Deng, Yanfei (State Key Laboratory of Conservation and Untilization of Subtropical Agro-Bioresources, Guangxi University) ;
  • Lei, Xiaocan (State Key Laboratory of Conservation and Untilization of Subtropical Agro-Bioresources, Guangxi University) ;
  • Luo, Chan (State Key Laboratory of Conservation and Untilization of Subtropical Agro-Bioresources, Guangxi University) ;
  • Huang, Ben (State Key Laboratory of Conservation and Untilization of Subtropical Agro-Bioresources, Guangxi University) ;
  • Shi, Deshun (State Key Laboratory of Conservation and Untilization of Subtropical Agro-Bioresources, Guangxi University)
  • Received : 2015.07.13
  • Accepted : 2015.11.14
  • Published : 2016.10.01
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Abstract

Isolation and culture of spermatogonial stem cells (SSCs) are attractive for production of genetic modified offspring. In the present study, buffalo spermatogonial stem-like cells were isolated, cultured and expression pattern of different germ cell marker genes were determined. To recover spermatogonia, testes from age 3 to 7 months of buffalo were decapsulated, and seminiferous tubules were enzymatically dissociated. Two types of cells, immature sertoli cell and type A spermatogonia were observed in buffalo testes in this stage. Germ cell marker genes, OCT3/4 (Pou5f1), THY-1, c-kit, PGP9.5 (UCHL-1) and Dolichos biflorus agglutinin, were determined to be expressed both in mRNA and protein level by reverse transcription polymerase chain reaction and immunostaining in buffalo testes and buffalo spermatogonial stem-like cells, respectively. In the following, when the isolated buffalo buffalo spermatogonial stem-like cells were cultured in the medium supplemented 2.5% fetal bovine serum and 40 ng/mL glial cell-derived neurotrophic factor medium, SSCs proliferation efficiency and colony number were significantly improved than those of other groups (p<0.05). These findings may help in isolation and establishing long term in vitro culture system for buffalo spermatogonial stem-like cells, and accelerating the generation of genetic modified buffaloes.

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