The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Changes of Mortality, Oxygen Consumption Rate and Organ Structure in the Oyster Crassostrea gigas Exposed to Lead

납 (Pb) 에 노출된 굴 Crassostrea gigas의 사망률, 산소소비율 및 기관계 구조의 변화

  • Shin, Yun Kyung (Southeast Sea Fisheries Research Institute, NIFS) ;
  • Ju, Sun Mi (Department of Aqualife Medicine, Chonnam National University) ;
  • Park, Jung Jun (South Sea Fisheries Research Institute, NIFS) ;
  • Jin, Young Guk (South Sea Fisheries Research Institute, NIFS) ;
  • Lim, Hyun Sig (Department of Marine and Fisheries Resources, Mokpo National University) ;
  • Lee, Jung Sick (Department of Aqualife Medicine, Chonnam National University)
  • 신윤경 (국립수산과학원 남동해수산연구소) ;
  • 주선미 (전남대학교 수산생명의학과) ;
  • 박정준 (국립수산과학원 남해수산연구소) ;
  • 진영국 (국립수산과학원 남해수산연구소) ;
  • 임현식 (목포대학교 해양수산자원학과) ;
  • 이정식 (전남대학교 수산생명의학과)
  • Received : 2016.02.16
  • Accepted : 2016.03.30
  • Published : 2016.03.31
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Abstract

This study was conducted to find out biological response of the oyster Crassostrea gigas exposed to lead (Pb). Experimental period was four weeks. Experimental groups were composed of one control condition and three lead exposure conditions (0.20, 0.40 and 0.80 mg/L). The results of the study confirmed that lead induces reduction of survival rate and oxygen consumption rate and degeneration of organ structure of the bivalve. Oxygen consumption rate was observed exposure groups lower than control decline by 15%-55%. Histological analysis of organ system illustrated expansion of hemolymph sinus, degeneration of epithelial layer and connective tissue layer of the mantle. Also, epithelial degeneration and disappearance of cilia band are recognized in the gill filament and it was observed disruption of epithelial layer and degeneration of basophilic cell and epithelial cell in the digestive tubules.

본 연구는 납에 노출된 굴 Crassostrea gigas의 생물학적 반응을 알아보고자 하였다. 실험기간은 4주였으며, 실험구는 대조구 1개와 납 노출구 3개 (0.20, 0.40 and 0.80 mg/L) 였다. 실험 결과 납은 굴의 생존율과 산소소비율의 저하 및 기관계의 조직학적 변성을 유도하는 것이 확인되었다. 산소소비율은 대조구에 비해 납 노출구에서 15-55% 감소하였다. 기관계의 조직학적 분석 결과, 외투막에서는 혈림프동의 팽창, 상피층과 결합조직층의 변성을 보였다. 아가미에서는 새엽 상피세포의 변성과 섬모대의 소실이 확인 되었으며, 소화선세관에서는 상피세포와 호염기성세포의 변성 및 상피층의 파괴가 관찰되었다.

Keywords

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