The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Epidemiology and Control of Strawberry Bacterial Angular Leaf Spot Disease Caused by Xanthomonas fragariae

  • Kim, Da-Ran (Dvision of Applied Life Science, Gyeongsang National University) ;
  • Gang, Gun-hye (Department of Plant Medicine and Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Jeon, Chang-Wook (Dvision of Applied Life Science, Gyeongsang National University) ;
  • Kang, Nam Jun (Department of Agricultural Plant Science and Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Lee, Sang-woo (Department of Horticulture, Gyeongnam National University of Science and Technology) ;
  • Kwak, Youn-Sig (Department of Plant Medicine and Institute of Agriculture & Life Sciences, Gyeongsang National University)
  • Received : 2016.01.05
  • Accepted : 2016.03.02
  • Published : 2016.08.01
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Abstract

Strawberry bacterial angular leaf spot (ALS) disease, caused by Xanthomonas fragariae has become increasingly problematic in the strawberry agro-industry. ALS causes small angular water-soaked lesions to develop on the abaxial leaf surface. Studies reported optimum temperature conditions for X. fragariae are $20^{\circ}C$ and the pathogen suffers mortality above $32^{\circ}C$. However, at the nursery stage, disease symptoms have been observed under high temperature conditions. In the present study, results showed X. fragariae transmission was via infected maternal plants, precipitation, and sprinkler irrigation systems. Systemic infections were detected using X. fragariae specific primers 245A/B and 295A/B, where 300-bp and 615-bp were respectively amplified. During the nursery stage (from May to August), the pathogen was PCR detected only in maternal plants, but not in soil or irrigation water through the nursery stage. During the cultivation period, from September to March, the pathogen was detected in maternal plants, progeny, and soil, but not in water. Additionally, un-infected plants, when planted with infected plants were positive for X. fragariae via PCR at the late cultivation stage. Chemical control for X. fragariae with oxolinic acid showed 87% control effects against the disease during the nursery period, in contrast to validamycin-A, which exhibited increased efficacy against the disease during the cultivation stage (control effect 95%). To our knowledge, this is the first epidemiological study of X. fragariae in Korean strawberry fields.

Keywords

References

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