Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Detection and Distribution of Fungal Vector P. graminis of BaYMV

보리누른모자이크병 매개곰팡이(Polymyxa graminis) 검정 및 분포현황

  • 이봉춘 (농촌진흥청 국립식량과학원) ;
  • 김상민 (농촌진흥청 국립식량과학원) ;
  • 배주영 (농촌진흥청 국립식량과학원) ;
  • 나지은 (농촌진흥청 국립식량과학원) ;
  • 김선림 (농촌진흥청 국립식량과학원) ;
  • 김강민 (전북대학교 생명공학부) ;
  • 이중환 (경상북도 농업기술원 생물자원연구소)
  • Received : 2016.04.05
  • Accepted : 2016.07.05
  • Published : 2016.08.31
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Abstract

Barley yellow mosaic virus (BaYMV) is transmitted by a root-inhabiting Polymyxa graminis (P. graminis) and thus the disease is called "soil-borne". In this study, the presence of P. graminis was confirmed by PCR test based on specific sequences of P. graminis type I (P. graminis f. sp. temperata) Internal Transcribed Spacer (ITS). P. graminis was detected in the BaYMV infected soil and root of barley plants. The monitoring of P. graminis was conducted in March 2015 in 8 korean provinces. It was detected in the soil of all collected regions. This is the first report on a P. graminis a survey of Korea.

보리누른모자이크병은 토양전염성 곰팡이인 Polymyxa graminis (P. graminis)에 의해 매개된다. 본 연구에서는 BaYMV 이병토양 및 이병주 뿌리로부터 P. graminis의 PCR 검정방법을 확립하였다. P. graminis type I (P. graminis f.sp. temperata) Internal Transcribed Spacer (ITS) 영역의 염기서열 특이적인 primer를 제작하고, 이병토양 및 이병주의 뿌리와 잎으로부터 DNA를 추출하여 PCR 검정을 실시하였다. 결과 P. graminis는 이병토양 및 이병주 뿌리로부터 검출되었다. 남부지역의 대표적인 보리 재배지역 8개 지역으로부터 이병토양을 채집하여 PCR 검정에 의해 P. graminis의 분포상황을 조사하였다. 결과 조사한 지역 전체에서 P. graminis의 분포가 확인되었다.

Keywords

References

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