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Bitter Melon (Momordica charantia) Extract Enhances Exercise Capacity in Mouse Model

여주(Momordica charantia) 추출물이 생쥐의 지구력 운동수행능력 향상 효과에 미치는 영향

  • Received : 2016.02.29
  • Accepted : 2016.08.17
  • Published : 2016.08.31

Abstract

Bitter melon (Momordica charantia) is used in traditional herbal medicine in many Asian countries for the treatment of several diseases such as diabetes, eczema, night blindness, psoriasis, and rheumatism. Especially, most reports concerning the biological activities of bitter melon have focused on its effects on diabetes and hyperglycemia. Also, bitter melon is regarded as a longevity food, suggesting that it has several beneficial effects on anti-aging and the maintenance of a healthy state. Thus, we investigated whether bitter melon could increase the capacity of exercise in this study. Interestingly, bitter melon fruit extract activated AMP-activated protein kinase (AMPK), which is important for regulating glucose homeostasis, mitochondrial content and exercise capacity. In addition, bitter melon extract increased the expression of enzymes involved in fatty acid oxidation such as mitochondrial uncoupling protein 3 (UCP3), carnitine palmitoyl transferase 1b (CPT1b), and pyruvate dehydrogenase lipoamide kinase isozyme 4 (PDK4). Moreover, exercise tolerance was much more enhanced in bitter melon treated animals compared to the non-treated control group. These results suggest that bitter melon is a promising candidate for the development of functional foods beneficial for physical strength and the enhancement of exercise capacity.

Keywords

References

  1. Abascal K, Yarnell E. 2005. Using bitter melon to treat diabetes. J Altern Complement Med 11:179-184 https://doi.org/10.1089/acm.2005.11.179
  2. Ali L, Khan AKA, Mamun MIR, Mosihuzzaman M, Nahar N, Alam MN, Rokeya B. 1993. Studies on hypoglycemic effects of fruit pulp, seed, and whole plant of Momordica charantia on normal and diabetic model rats. Planta Med 59:408-412 https://doi.org/10.1055/s-2006-959720
  3. Andersson U, Filipsson K, Abbott CR, Woods A, Smith K, Bloom SR, Carling D, Small CJ. 2004. AMP-activated protein kinase plays a role in the control of food intake. J Biol Chem 279:12005-12008 https://doi.org/10.1074/jbc.C300557200
  4. Baur JA, Pearson KJ, Price NL, Jamieson HA, Lerin C, Kalra A, Prabhu VV, Allard JS, Lopez-Lluch G, Lewis K, Pistell PJ, Poosala S, Becker KG, Boss O, Gwinn D, Wang M, Ramaswamy S, Fishbein KW, Spencer RG, Lakatta EG, Le Couteur D, Shaw RJ, Navas P, Puigserver P, Ingram DK, de Cabo R, Sinclair DA. 2006. Resveratrol improves health and survival of mice on a high-calorie diet. Nature 444: 337-342 https://doi.org/10.1038/nature05354
  5. Beloin N, Gbeassor M, Akpagana K, Hudson J, De Soussa K, Koumaglo K, Arnason JT. 2005. Ethnomedicinal uses of Momordica charantia (Cucurbitaceae) in Togo and relation to its phytochemistry and biological activity. J Ethnopharmacol 96:49-55 https://doi.org/10.1016/j.jep.2004.08.009
  6. Cefalu WT, Ye J, Wang ZQ. 2008. Efficacy of dietary supplementation with botanicals on carbohydrate metabolism in humans. Endocr Metab Immune Disord Drug Targets 8:78-81 https://doi.org/10.2174/187153008784534376
  7. Chen Q, Chan LLY, Li ETS. 2003. Bitter melon (Momordica charantia) reduces adiposity, lowers serum insulin and normalizes glucose tolerance in rats fed a high fat diet. J Nutr 133:1088-1093 https://doi.org/10.1093/jn/133.4.1088
  8. Cheng LY, Tang L, Yan F, Wang S, Chen F. 2004 The effects of the total saponin extract from the shoots of Momordica charantia L. on anti-virus HSV-II activity. J Sichuan Univ (Nat Sci Ed) 41:641-643
  9. Keppler D, Sameni M, Moin K, Sloane BF, Mikkelsen T, Diglio CA. 1996. Tumor progression and angiogenesis: cathepsin B & Co. Biochem Cell Biol 74:799-810 https://doi.org/10.1139/o96-086
  10. Divya D, Hettiarachchy NS, Ganesh V, Kanna A, Rayaprolu S. 2013. Phenolic extracts from leaves of bitter melon (Momordica charantia) with antioxidant properties. JASA 2:28-34 https://doi.org/10.14511/jasa.2013.020105
  11. Grover JK, Yadav SP. 2004. Pharmacological actions and potential uses of Momordica charantia: A review. J Ethnopharmacol 93:123-132 https://doi.org/10.1016/j.jep.2004.03.035
  12. Hardie DG. 2007. AMP-activated/SNF1 protein kinases: Conserved guardians of cellular energy. Nat Rev Mol Cell Biol 8: 774-785 https://doi.org/10.1038/nrm2249
  13. Hu QS, Yang YC, Xia H. 2004. Studies on inhibiting-bacteria of nature substance extracted from leaves and rattan of Momordica charantia Linn. Jiangxi Chemical Industry 2:017
  14. Jung HY, Lee AN, Song TJ, An HS, Kim YH, Kim KD, Kim IB, Kim KS, Han BS, Kim CH, Kim KS, Kim JB. 2012. Korean mistletoe (Viscum album coloratum) extract improves endurance capacity in mice by stimulating mitochondrial activity. J Med Food 15:621-628 https://doi.org/10.1089/jmf.2010.1469
  15. Kim SM, Lee YM, Kim mj, Nam SY, Kim SH, Jang HH. 2013. Effects of Agrimonia pilosa Ledeb. water extract on ${\alpha}$-glucosidase inhibition and glucose uptake in C2C12 skeletal muscle cells. Korean J Food and Nutr 26:806-813 https://doi.org/10.9799/ksfan.2013.26.4.806
  16. Kubola J, Siriamorpun S. 2008. Phenolic contents and antioxidant activities of bitter ground (Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro. Food Chem 110: 881-890 https://doi.org/10.1016/j.foodchem.2008.02.076
  17. Kubota N, Yano W, Kubota T, Yamauchi T, Itoh S, Kumagai H, Kozono H, Takamoto I, Okamoto S, Shiuchi T, Suzuki R, Satoh H, Tsuchida A, Moroi M, Sugi K, Noda T, Ebinuma H, Ueta Y, Kondo T, Araki E, Ezaki O, Nagai R, Tobe K, Terauchi Y, Ueki K, Minokoshi Y, Kadowaki T. 2007. Adiponectin stimulates AMP-activated protein kinase in the hypothalamus and increases food intake. Cell Metab 6:55-68 https://doi.org/10.1016/j.cmet.2007.06.003
  18. Lagouge M, Argmann C, Gerhart-Hines Z, Meziane H, Lerin C, Daussin F, Messadeq N, Milne J, Lambert P, Elliott P, Geny B, Laakso M, Puigserver P, Auwerx J. 2006. Resveratrol improves mitochondrial function and protects against metabolic disease by activating SIRT1 and PGC-1a. Cell 127: 1109-1122 https://doi.org/10.1016/j.cell.2006.11.013
  19. Lans CA. 2006. Ethnomedicines used in Trinidad and Tobago for urinary problems and diabetes mellitus. J Ethnobiol Ethnomed 2:45 https://doi.org/10.1186/1746-4269-2-45
  20. Lee HJ, Moon JH, Lee WM, Lee SG, Kim AK, Woo YH, Park DK. 2012. Charantin contents and fruit characteristics of bitter gourd (Momordica charantia L.) accessions. J Bio-Environ Control 21:379-384 https://doi.org/10.12791/KSBEC.2012.21.4.379
  21. Minokoshi Y, Alquier T, Furukawa N, Kim YB, Lee A, Xue B, Mu J, Foufelle F, Ferre P, Birnbaum MJ, Stuck BJ, Kahn BB. 2004. AMP-kinase regulates food intake by responding to hormonal and nutrient signals in the hypothalamus. Nature 428:569-574 https://doi.org/10.1038/nature02440
  22. Mu J, Brozinick JT Jr., Valladares O, Bucan M, Birnbaum MJ. 2001. A role for AMP-activated protein kinase in contractionand hypoxiaregulated glucose transport in skeletal muscle. Mol Cell 7:1085-1094 https://doi.org/10.1016/S1097-2765(01)00251-9
  23. Narkar VA, Downes M, Yu RT, Embler E, Wang YX, Banayo E, Mihaylova MM, Nelson MC, Zou Y, Juguilon H, Kang H, Shaw RJ, Evans RM. 2008. AMPK and PPARdelta agonists are exercise mimetics. Cell 134:405-415 https://doi.org/10.1016/j.cell.2008.06.051
  24. Nerurkar PV, Lee YK, Motosue M, Adeli K, Nerurkar VR. 2008. Momordica charantia (bitter melon) reduces plasma apolipoprotein B-100 and increases hepatic insulin receptor substrate and phosphoinositide-3 kinase interactions. Br J Nutr 100: 751-759
  25. Park CE, Kim MJ, Lee JH, Min BI, Bae H, Choe W, Kim SS, Ha J. 2007. Resveratrol stimulates glucose transport in C2C12 myotubes by activating AMP-activated protein kinase. Exp Mol Med 39:222-229 https://doi.org/10.1038/emm.2007.25
  26. Parkash A, Ng TB, Tso WW. 2002. Purification and characterization of charantin, a napin-like ribosome-inactivating peptide from bitter gourd (Momordica charantia) seeds. J Pept Res 59:197-202 https://doi.org/10.1034/j.1399-3011.2002.00978.x
  27. Rathi SS, Grover JK, Vats V. 2002. The effect of Momordica charantia and Mucuna pruriens in experimental diabetes and their effect on key metabolic enzymes involved in carbohydrate metabolism. Phytother Res 16:236-243 https://doi.org/10.1002/ptr.842
  28. Ray RB, Raychoudhuri A, Steele R, Nerurkar P. 2010. Bitter melon (Momordica charantia) extract inhibits breast cancer cell proliferation by modulating cell cycle regulatory genes and promotes apoptosis. Cancer Res 70:1925-1931 https://doi.org/10.1158/0008-5472.CAN-09-3438
  29. Schmourlo G, Mendonca-Filho RR, Alviano CS, Costa SS. 2005. Screening of antifungal agents using ethanol precipitation and bioautography of medicinal and food plants. J Ethnopharmacol 96:563-568 https://doi.org/10.1016/j.jep.2004.10.007
  30. Srivastava Y, Venkatadrishna-Bhatt H, Verma Y, Venkaiah K, Raval BH. 1993. Antidiabetic and adaptogenic properties of Momordica charantia extract: and experimental and clinical evaluation. Phytother Res 7:285-289 https://doi.org/10.1002/ptr.2650070405
  31. Thomson DM, Porter BB, Tall JH, Kim HJ, Barrow JR, Winder WW. 2007. Skeletal muscle and heart LKB1 deficiency causes decreased voluntary running and reduced muscle mitochondrial marker enzyme expression in mice. Am J Physiol Endocrinol Metab 292:E196-E202 https://doi.org/10.1152/ajpendo.00366.2006
  32. Yoon BR, Lee YJ, Hong HD, Lee YC, Kim YC, Rhee YK, Kim KT, Lee OH. 2012. Inhibitory effects of Panax ginseng C. A. Mayer treated with high temperature and high pressure on oxidative stress. Korean J Food and Nutr 25:800-806 https://doi.org/10.9799/ksfan.2012.25.4.800
  33. Zhang RQ, Ma LL, Lu JH, Hu YQ, Yu JF. 2003. A bacteriostatic test of leaf of Momordica charantia L. J Gannan Med Coll 23:272-273

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