The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Evaluation of the effects of a low dose of Asiasari radix on stem cell morphology and proliferation

  • Park, Jun-Beom (Department of Periodontics, College of Medicine, The Catholic University of Korea) ;
  • Lee, Ji-Eun (Department of Periodontics, College of Medicine, The Catholic University of Korea) ;
  • Jin, Seong-Ho (Department of Periodontics, College of Medicine, The Catholic University of Korea) ;
  • Ko, Youngkyung (Department of Periodontics, College of Medicine, The Catholic University of Korea) ;
  • Jeong, Su-Hyeon (Department of Rehabilitation Medicine of Korean Medicine, Chungju Hospital of Korean Medicine, College of Korean Medicine, Semyung University)
  • Received : 2016.05.26
  • Accepted : 2016.06.24
  • Published : 2016.06.30
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Abstract

Objectives: Asiasari radix (A. radix) is a traditional herb medicine that has been used as an analgesic, antitussive, or anti-allergic remedy. This study was performed to evaluate the effects of low concentration of the Asiasarum heterotropoides extract on the morphology and proliferation of the human mesenchymal stem cells derived from periodontal tissue. Methods: Stem cells derived from gingiva were grown in the presence of A. radix at final concentrations that ranged from 0.001 to $0.01{\mu}g/mL$. The morphology of the cells was viewed under an inverted microscope and the analysis of cell proliferation was performed by using Cell Counting Kit-8 (CCK-8) on days 1 and 3. Results: The control group showed fibroblast morphology. The shapes of the cells in 0.001 and $0.01{\mu}g/mL$ of A. radix were similar to that of the untreated control group. The cultures growing in the presence of A. radix at day 1 showed an increase in the CCK-8 value. The relative values of CCK-8 assays of 0.001 and $0.01{\mu}g/mL$ of A. radix are $130.6%{\pm}1.8%$ and $129.3%{\pm}1.5%$, respectively, when the CCK-8 result of the untreated control group at day 1 is considered 100% (P = 0.051). Conclusions: Within the limits of this study, low concentrations of A. radix seemed to increase the proliferation of the stem cells that were derived from the gingiva and did not have adverse effects on the morphology of the cells.

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