대한한의학회지 (The Journal of Korean Medicine)

  • 제37권2호
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  • Pages.76-84
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  • 2016
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  • 1010-0695(pISSN)
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  • 2288-3339(eISSN)
대한한의학회 (The Society of Korean Medicine)
권호 표지
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Rh2-enriched Korean Ginseng Ameliorates Chronic Fatigue in a Forced Exercise mouse model

  • Kim, Won-Yong (Liver and Immunology Research Center, Oriental Medical Collage of Daejeon University) ;
  • Lee, Jin-Seok (Liver and Immunology Research Center, Oriental Medical Collage of Daejeon University) ;
  • Yoo, Hwa-Seung (East West Cancer Center, Dunsan Korean Medicine Hospital, Daejeon University) ;
  • Son, Chang-Gue (Liver and Immunology Research Center, Oriental Medical Collage of Daejeon University)
  • 투고 : 2016.05.16
  • 심사 : 2016.06.22
  • 발행 : 2016.06.30
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초록

Objectives: We evaluated the anti-fatigue effects of Rh2-enriched Korean ginseng (Ginseng Rh2+) using a forced exercise-induced chronic fatigue mouse model. Methods: ICR male mice were subjected to running wheel for 1 h, 5 days/week during 4 weeks, and running velocity was gradually increased. Each running session was followed by oral administration of distilled water, Ginseng Rh2+ (150 or 300 mg/kg), or N-acetyl-L-cysteine (NAC, 100 mg/kg) 1 h later. The exercise tolerance and forced swimming test were performed to evaluate the fatigue condition. Results: Chronic forced exercise reduced the physical activity, as evidenced by the behavioral tests, which were notably ameliorated by Ginseng Rh2+ treatment. Ginseng Rh2+ treatment also attenuated the alterations of energy metabolism and oxidative stress in skeletal muscle tissues and/or sera, including malondialdehyde (MDA), lactate concentration and its related factors (lactate dehydrogenase, blood urea nitrogen, and glucose levels). Conclusion: These findings strongly suggest that Ginseng Rh2+ exerts a potent anti-fatigue effect through modulation of energy metabolism and oxidative response.

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참고문헌

  1. Coon JT, Ernst E. Panax ginseng: a systematic review of adverse effects and drug interactions. Drug Saf. 2002;25(5):323-44. https://doi.org/10.2165/00002018-200225050-00003
  2. Lee SM, Bae BS, Park HW, Ahn NG, Cho BG, Cho YL, et al. Characterization of Korean Red Ginseng (Panax ginseng Meyer): History, preparation method, and chemical composition. J Ginseng Res. 2015;39(4):384-91. https://doi.org/10.1016/j.jgr.2015.04.009
  3. Kaneko H, Nakanishi K. Proof of the mysterious efficacy of ginseng: basic and clinical trials: clinical effects of medical ginseng, korean red ginseng: specifically, its anti-stress action for prevention of disease. J Pharmacol Sci. 2004; 95(2):158-62. https://doi.org/10.1254/jphs.FMJ04001X5
  4. Hong YJ, Kim N, Lee K, Hee SC, Eun LJ, Tae KS, et al. Korean red ginseng (Panax ginseng) ameliorates type 1 diabetes and restores immune cell compartments. J Ethnopharmacol. 2012;144(2): 225-33. https://doi.org/10.1016/j.jep.2012.08.009
  5. Park BJ, Lee YJ, Lee HR, Jung DH, Na HY, Kim HB, et al. Effects of Korean Red Ginseng on Cardiovascular Risks in Subjects with Metabolic Syndrome: a Double-blind Randomized Controlled Study. Korean J Fam Med. 2012; 33(4):190-6. https://doi.org/10.4082/kjfm.2012.33.4.190
  6. Helms S. Cancer prevention and therapeutics: Panax ginseng. Altern Med Rev. 2004;9(3): 259-74.
  7. Son CG. Review of the Prevalence of Chronic Fatigue Worldwide. J Kor Ori Med. 2012;33(2): 25-33.
  8. Norheim KB, Jonsson G, Omdal R. Biological mechanisms of chronic fatigue. Rheumatology (Oxford). 2011;50(6):1009-18. https://doi.org/10.1093/rheumatology/keq454
  9. Myhill S, Booth NE, McLaren-Howard J. Chronic fatigue syndrome and mitochondrial dysfunction. Int J Clin Exp Med. 2009;2(1): 1-16.
  10. Allen DG. Lamb GD. Westerblad H. Skeletal muscle fatigue: cellular mechanisms. Physiol Rev. 2008;88(1):287-332. https://doi.org/10.1152/physrev.00015.2007
  11. Ament W, Verkerke GJ. Exercise and fatigue. Sports Med. 2009;39(5):389-422. https://doi.org/10.2165/00007256-200939050-00005
  12. Kristensen M, Albertsen J, Rentsch M, Juel C. Lactate and force production in skeletal muscle. J Physiol. 2005;562(Pt 2):521-6. https://doi.org/10.1113/jphysiol.2004.078014
  13. Hwang JT, Kim SH, Lee MS, Kim SH, Yang HJ, Kim MJ. Anti-obesity effects of ginsenoside Rh2 are associated with the activation of AMPK signaling pathway in 3T3-L1 adipocyte. Biochem Biophys Res Commun. 2007;364(4): 1002-8. https://doi.org/10.1016/j.bbrc.2007.10.125
  14. Choi K, Kim M, Ryu J, Choi C. Ginsenosides compound K and Rh(2) inhibit tumor necrosis factor-alpha-induced activation of the NF-kappaB and JNK pathways in human astroglial cells. Neurosci Lett. 2007;421(1):37-41. https://doi.org/10.1016/j.neulet.2007.05.017
  15. An IS, An S, Kwon KJ, Kim YJ, Bae S. Ginsenoside Rh2 mediates changes in the microRNA expression profile of human non-small cell lung cancer A549 cells. Oncol Rep. 2013;29(2):523-8. https://doi.org/10.3892/or.2012.2136
  16. Li B, Zhao J, Wang CZ, Searle J, He TC, Yuan CS, et al. Ginsenoside Rh2 induces apoptosis and paraptosis-like cell death in colorectal cancer cells through activation of p53. Cancer Lett. 2011;301(2):185-92 https://doi.org/10.1016/j.canlet.2010.11.015
  17. Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978;86(1):271-8. https://doi.org/10.1016/0003-2697(78)90342-1
  18. Baeg IH, So SH. The world ginseng market and the ginseng (Korea). J Ginseng Res. 2013;37 (1):1-7. https://doi.org/10.5142/jgr.2013.37.1
  19. Yang Z, Zhao T, Liu H, Zhang L. Ginsenoside Rh2 inhibits hepatocellular carcinoma through ${\beta}$-catenin and autophagy. Sci Rep. 2016;6:19383. https://doi.org/10.1038/srep19383
  20. Park EK, Lee EJ, Lee SH, Koo KH, Sung JY, Hwang EH, et al. Induction of apoptosis by the ginsenoside Rh2 by internalization of lipid rafts and caveolae and inactivation of Akt. Br J Pharmacol. 2010;160(5):1212-23. https://doi.org/10.1111/j.1476-5381.2010.00768.x
  21. Li LC, Piao HM, Zheng MY, Lin ZH, Choi YH, Yan GH. Ginsenoside Rh2 attenuates allergic airway inflammation by modulating nuclear factor-${\kappa}$B activation in a murine model of asthma. Mol Med Rep. 2015;12(5):6946-54. https://doi.org/10.3892/mmr.2015.4272
  22. Wang H, Yu P, Gou H, Zhang J, Zhu M, Wang ZH, et al. Cardioprotective Effects of 20(S)-Ginsenoside Rh2 against Doxorubicin-Induced Cardiotoxicity In Vitro and In Vivo. Evid Based Complement Alternat Med. 2012;2012, 506214.
  23. Qiu J, Li W, Feng SH, Wang M, He ZY. Ginsenoside Rh2 promotes nonamyloidgenic cleavage of amyloid precursor protein via a cholesterol-dependent pathway. Genet Mol Res. 2014;13(2):3586-98. https://doi.org/10.4238/2014.May.9.2
  24. Kim YJ, Jeon JN, Jang MG, Oh JY, Kwon WS, Jung SK, et al. Ginsenoside profiles and related gene expression during foliation in Panax ginseng Meyer. J Ginseng Res. 2014;38(1): 66-72. https://doi.org/10.1016/j.jgr.2013.11.001
  25. Romer LM, Polkey MI. Exercise-induced respiratory muscle fatigue: implications for performance. J Appl Physiol (1985). 2008;104 (3):879-88. https://doi.org/10.1152/japplphysiol.01157.2007
  26. Medved I, Brown MJ, Bjorksten AR, Murphy KT, Petersen AC, Sostaric S, et al. N-acetylcysteine enhances muscle cysteine and glutathione availability and attenuates fatigue during prolonged exercise in endurance-trained individuals. J Appl Physiol (1985). 2004;97(4):1477-85. https://doi.org/10.1152/japplphysiol.00371.2004
  27. Fulcher KY, White PD. Strength and physiological response to exercise in patients with chronic fatigue syndrome. J Neurol Neurosurg Psychiatry. 2000;69(3):302-7. https://doi.org/10.1136/jnnp.69.3.302
  28. van Vulpen JK, Peeters PH, Velthuis MJ, van der Wall E, May AM. Effects of physical exercise during adjuvant breast cancer treatment on physical and psychosocial dimensions of cancer-related fatigue: A meta-analysis. Maturitas. 2016;85:104-11. https://doi.org/10.1016/j.maturitas.2015.12.007
  29. Gosker HR. Schols AM. Fatigued muscles in COPD but no finishing line in sight. Eur Respir J. 2008;31(4):693-4. https://doi.org/10.1183/09031936.00015308
  30. Egan B, Zierath JR. Exercise metabolism and the molecular regulation of skeletal muscle adaptation. Cell Metab. 2013;17(2):162-84. https://doi.org/10.1016/j.cmet.2012.12.012
  31. Reid MB. Free radicals and muscle fatigue: Of ROS, canaries, and the IOC. Free Radic Biol Med. 2008;44(2):169-79. https://doi.org/10.1016/j.freeradbiomed.2007.03.002
  32. Steinbacher P, Eckl P. Impact of oxidative stress on exercising skeletal muscle. Biomolecules. 2015;5(2):356-77. https://doi.org/10.3390/biom5020356

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