Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Enhancement of Anti-Obesity Activities of Aronia melanocarpa Elliot Extracts from Low Temperature Ultrasonification Process

아로니아 저온 초음파 추출물의 항비만 활성 증진

  • Kim, Nam Young (Department of Medical Biomaterials Engineering, Kangwon National University) ;
  • Lee, Jeong Min (Department of Biochemistry, Hallym University Medical School) ;
  • Lee, Jae Yong (Department of Biochemistry, Hallym University Medical School) ;
  • Lee, Hyeon Yong (Department of Food Science and Engineering, Seowon University)
  • 김남영 (강원대학교 생물의소재공학과) ;
  • 이정민 (한림대학교 의과대학 생화학교실) ;
  • 이재용 (한림대학교 의과대학 생화학교실) ;
  • 이현용 (서원대학교 식품공학과)
  • Received : 2016.04.20
  • Accepted : 2016.07.15
  • Published : 2016.08.30
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Abstract

Background: This study represents the first report that the anti-obesity activity of ethanol extracts of Aronia melanocarpa can be enhanced through ultrasonification at a frequency of 120 kHz at $60^{\circ}C$ (UE). Methods and Results: The amounts of cyanidin-3-O-galactose (cya-gal), a major anthocyanin in A. melanocarpa were higher by up to 402.4 mg/100 g, as compared with 221.4 mg/100 g and 322.1 mg/100 g, for hot water at $100^{\circ}C$ and 70% ethanol at $80^{\circ}C$ respectively. This result should cause the higher antioxidant activities of the UE than extract of hot water and ethanol in DPPH free radical scavenging. It was confirmed that the high antioxidant activity of UE could play an important role in inhibiting the production of proteins related to adipocyte differentiation, such as peroxisome proliferator activated receptor-${\gamma}$ (PPAR-${\gamma}$) and sterol regulatory element binding protein 1 (SREBP1). Conclusions: Ultrasonification at a frequency of 120 kHz at $60^{\circ}C$ should result in better anti-obesity activity than that observed using other processes. It was also observed for the first time that the anti-obesity activity of A. melanocarpa was associated with its antioxidant activity, possibly due to the higher elution of intact cya-gal, owing to efficient low temperature ultrasonification extraction. These results could also be applied to improve other biological activities of medicinal herbs that contain many types of heat-labile bioactive substances.

Keywords

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