Improved axonal regeneration by Boyanghwano-tang treatment in mice given sciatic nerve injury

좌골신경 손상 모델에서 보양환오탕 처리에 의한 축삭 재생반응성 분석

  • Chang, In-Ae (Dept. of Neurophysiology, College of Oriental Medicine, Daejeon University) ;
  • Kim, Ki-Joong (Dept. of Neurophysiology, College of Oriental Medicine, Daejeon University) ;
  • Namgung, Uk (Dept. of Neurophysiology, College of Oriental Medicine, Daejeon University)
  • Received : 2016.07.04
  • Accepted : 2016.08.01
  • Published : 2016.08.20

Abstract

While axons in the peripheral nerve can regenerate and lead to functional recovery to a certain extent after injury, its efficacy varies depending on the severity and duration of the injury. Here, we investigated the effects of Boyanghwano-tang (BYHOT) treatment on the regenerative responses in the sciatic nerves after prolonged transection and coaptation surgery. In mice given crush injury, axonal regeneration was completed when analyzed 1 week later and did not show any difference in regenerative reponses in the distal portion of the nerve between saline- and BYHOT-treated groups. In animal models with transection and reconnection, axonal regeneration was markedly retarded compared to animals with crush injury. Regenerating axons were extended into the reconnected distal portion of the nerve more actively in animals treated with BYHOT than saline controls. Cdc2 protein was similarly induced in nerves with crush injury and with transection and recollection, and its level was lower in BYHOT-treated animal than saline control when measured 2 weeks after nerve reconnection. These results suggest that BYHOT may be useful to promote axonal regeneration in the peripheral nerve after severe injury.

Keywords

References

  1. Chen ZL, Yu WM, Strickland S. Peripheral regeneration. Annu Rev Neurosci. 2007;30:209-33. https://doi.org/10.1146/annurev.neuro.30.051606.094337
  2. 한창호, 박용기. "가미보양환오탕이 뇌허혈모델에서 신경세포보호를 통해 뇌경색억제에 미치는 효과", , 대한침구의학회지 2010 8월 27(4):29-38
  3. Jinglong T, Weijuan G, Jun L, Tao Q, Hongbo Z, Shasha L. The molecular and electrophysiological mechanism of buyanghuanwu decoction in learning and memory ability of vascular dementia rats. Brain Res Bull. 2013 Oct;99:13-8. https://doi.org/10.1016/j.brainresbull.2013.09.002
  4. Li XM, Bai XC, Qin LN, Huang H, Xiao ZJ, Gao TM. Neuroprotective effects of Buyang Huanwu Decoction on neuronal injury in hippocampus after transient forebrain ischemia in rats. Neurosci Lett. 2003 Jul 31;346(1-2):29-32. https://doi.org/10.1016/S0304-3940(03)00522-6
  5. Yang S, Gao Q, Xing S, Feng X, Peng L, Dong H, Bao L, Zhang J, Hu Y, Li G, Song T, Li Z, Sun J. Neuroprotective effects of Buyang Huanwu decoction against hydrogen peroxide induced oxidative injury in Schwann cells. J Ethnopharmacol. 2011 Oct 11;137(3) :1095-101. https://doi.org/10.1016/j.jep.2011.07.014
  6. Chen A, Wang H, Zhang J, Wu X, Liao J, Li H, Cai W, Luo X, Ju G. BYHWD rescues axotomized neurons and promotes functional recovery after spinal cord injury in rats. J Ethnopharmacol. 2008 May 22;117(3):451-6. https://doi.org/10.1016/j.jep.2008.02.029
  7. Chang IA, Oh MJ, Kim MH, Park SK, Kim BG, Namgung U. Vimentin phosphorylation by Cdc2 in Schwann cell controls axon growth via ${\beta}1$-integrin activation. FASEB J. 2012 Jun;26(6): 2401-13. https://doi.org/10.1096/fj.11-199018
  8. Han IS, Seo TB, Kim KH, Yoon JH, Yoon SJ, Namgung U. Cdc2-mediated Schwann cell migration during peripheral nerve regeneration. J Cell Sci. 2007 Jan 15;120(Pt 2):246-55. https://doi.org/10.1242/jcs.03322