Mycobiology

The Korean Society of Mycology (한국균학회)
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Phylogenetic and Morphological Identification of the Novel Pathogen of Rheum palmatum Leaf Spot in Gansu, China

  • Wang, Yan (Gansu University of Chinese Medicine) ;
  • Charkowski, Amy O. (Department of Plant Pathology, University of Wisconsin-Madison) ;
  • Zeng, Cuiyun (Gansu University of Chinese Medicine) ;
  • Zhu, Tiantian (Gansu University of Chinese Medicine) ;
  • Wang, Huizhen (Gansu University of Chinese Medicine) ;
  • Chen, Honggang (Gansu University of Chinese Medicine)
  • Received : 2016.04.26
  • Accepted : 2016.06.07
  • Published : 2016.06.30
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Abstract

A new leaf spot disease was observed on leaves of Rheum palmatum (Chinese rhubarb) in Northwest China (Gansu Province) starting in 2005. A Septoria-like fungus was isolated and completion of Koch's postulates confirmed that the fungus was the casual agent of the leaf spot disease. Morphology and molecular methods were combined to identify the pathogen. The fungus produced conidiomata pycnidia and the conidia were 2~5 septate, $61.2{\sim}134.1{\mu}m$ in length and $3.53{\sim}5.3{\mu}m$ in width, which is much larger than the known Spetoria species that infects Polygonaceae species. Phylogenic analysis of the internal transcribed spacer region confirmed that this Septoria-like fungus is within the Septoria genus but distinct from known Septoria species. Together, these morphological and phylogenetic data support that the R. palmatum infecting Septoria strain is a newly-described plant pathogenic species.

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References

  1. Chinese Pharmacopoeia Commission. Pharmacopoeia of the People's Republic of China, Vol. 1. Beijing: Medical Science and Technology Press; 2010. p. 17.
  2. Kang SC, Lee CM, Choi H, Lee JH, Oh JS, Kwak JH, Zee OP. Evaluation of oriental medicinal herbs for estrogenic and antiproliferative activities. Phytother Res 2006;20:1017-9. https://doi.org/10.1002/ptr.1987
  3. Lee EH, Kim JM, Kim C. Pharmacokinetic analysis of rhein in Rheum undulatum L. J Ethnopharmacol 2003;84:5-9. https://doi.org/10.1016/S0378-8741(02)00222-2
  4. Bai JK. Flora Fungorum Sinicorum. Vol. 17. Sphaeropsidales, Ascochyta, Septoria. Beijing: Science Press; 2003.
  5. Guo L. Flora Fungorum Sinicorum. Vol. 12. Ustilaginaceae. Beijing: Science Press; 2000.
  6. Zhuang JY. Flora Fungorum Sinicorum. Vol. 19. Uredinales (II). Beijing: Science Press; 2003.
  7. Bai JK. Flora Fungorum Sinicorum. Vol. 15. Sphaeropsidales, Phoma, Phyllosticta. Beijing: Science Press; 2000. p. 162-3.
  8. Zhuang WY. Fungi of northwestern China. Ithaca (NY): Mycotaxon, Ltd.; 2005.
  9. Wang Y, Chen X, Li Y. Survey and pathogen identification of rhubarb diseases in Gansu Province. Zhongguo Zhong Yao Za Zhi 2009;34:953-6.
  10. Liu W, Yan LY, Wen ZH, Chen XR. Survey of virus diseases on 18 species of medical herbs in Gansu Province and identification of two virus disease. Plant Prot 2014;40:133-7.
  11. Donk MA. Nomina conservanda proposita I. Proposals in fungi: Deuteromycetes. Regnum Veg 1964;34:7-15.
  12. Sutton BC. The Coelomycetes: fungi imperfecti with pycnidia, acervuli and stromata. Kew: Commonwealth Mycological Institute; 1980.
  13. Constantinescu O. Taxonomical revision of Septoria-like fungi parasitic on Betulaceae. Trans Br Mycol Soc 1984;83:383-98. https://doi.org/10.1016/S0007-1536(84)80035-2
  14. Farr DF. Species of Septoria on the Fabaceae, subfamily Faboideae, tribe Genisteae. Sydowia 1992;44:13-31.
  15. Verkley GJ, Starink-Willemse M. A phylogenetic study of some Septoria species pathogenic to Asteraceae based on ITS ribosomal DNA sequences. Mycol Prog 2004;3:315-23. https://doi.org/10.1007/s11557-006-0101-0
  16. Verkley GJ, Starink-Willemse M, van Iperen A, Abeln EC. Phylogenetic analyses of Septoria species based on the ITS and LSU-D2 regions of nuclear ribosomal DNA. Mycologia 2004;96:558-71. https://doi.org/10.1080/15572536.2005.11832954
  17. Priest MJ. Fungi of Australia: Septoria. Melbourne: CSIRO Publishing; 2006.
  18. von Arx JA. Mycosphaerella and its anamorphs. Proc K Ned Akad Wet C 1983;86:15-54.
  19. Crous PW, Aptroot A, Kang JC, Braun U, Wingfield MJ. The genus Mycosphaerella and its anamorphs. Stud Mycol 2000;45:107-21.
  20. Crous PW, Kang JC, Braun U. A phylogenetic redefinition of anamorph genera in Mycosphaerella based on ITS rDNA sequence and morphology. Mycologia 2001;93:1081-101. https://doi.org/10.2307/3761670
  21. Farr DF, Rossman AY. Fungal Databases. Washington, DC: Systematic Mycology and Microbiology Laboratory, ARS, USDA; 2015.
  22. White TJ, Bruns TD, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols, a guide to methods and applications. San Diego (CA): Academic Press; 1990. p. 315-22.
  23. Katoh K, Misawa K, Kuma K, Miyata T. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 2002;30:3059-66. https://doi.org/10.1093/nar/gkf436
  24. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999;41:95-8.
  25. Pierobom CR. Studies on pycnidial and ascocarpic fungi which cause leaf spots on wheat in Washington [dissertation]. Washington, DC: Washington State University; 1983.
  26. Kema GH, Annone JG. In vitro production of pycnidia by Septoria tritici. Neth J Plant Pathol 1991;97:65-72. https://doi.org/10.1007/BF01974270
  27. Verkley GJ, Priest MJ. Septoria and similar coelomycetous anamorphs of Mycosphaerella. Stud Mycol 2000;45:123-8.
  28. Beach WS. Biologic specialization in the genus Septoria. Am J Bot 1919;6:1-33. https://doi.org/10.2307/2435090
  29. Farr DF. Septoria species on Cornus. Mycologia 1991;83:611-23. https://doi.org/10.2307/3760216