Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Novel Function of Sprouty4 as a Regulator of Stemness and Differentiation of Embryonic Stem Cells

  • Lee, Jae-Young (Dept. of Biomedical Science, College of Life Science, CHA University) ;
  • Park, Sunghyun (Dept. of Biomedical Science, College of Life Science, CHA University) ;
  • Kim, Kwang-Soo (Dept. of Biomedical Science, College of Life Science, CHA University) ;
  • Ko, Jeong-Jae (Dept. of Biomedical Science, College of Life Science, CHA University) ;
  • Lee, Soohong (Dept. of Biomedical Science, College of Life Science, CHA University) ;
  • Kim, Keun Pil (Dept. of Life Science, Chung-Ang University) ;
  • Park, Kyung-Soon (Dept. of Biomedical Science, College of Life Science, CHA University)
  • Received : 2016.05.19
  • Accepted : 2016.06.10
  • Published : 2016.06.30
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Abstract

Sprouty (Spry) genes encode inhibitors of the receptor tyrosine kinase signaling cascade, which plays important roles in stem cells. However, the role of Spry4 in the stemness of embryonic stem cells has not been fully elucidated. Here, we used mouse embryonic stem cells (mESCs) as a model system to investigate the role of Spry4 in the stem cells. Suppression of Spry4 expression results in the decreases of cell proliferation, EB formation and stemness marker expression, suggesting that Spry4 activity is associated with stemness of mESCs. Teratoma assay showed that the cartilage maturation was facilitated in Spry4 knocked down mESCs. Our results suggest that Spry4 is an important regulator of the stemness and differentiation of mESCs.

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References

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