Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Prevalence of Enteric Protozoan Oocysts with Special Reference to Sarcocystis cruzi among Fecal Samples of Diarrheic Immunodeficient Patients in Iran

  • Agholi, Mahmoud (HIV/AIDS Research Center, Fasa University of Medical Sciences) ;
  • Shahabadi, Shahrbanou Naderi (Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences) ;
  • Motazedian, Mohammad Hossein (Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences) ;
  • Hatam, Gholam Reza (Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences)
  • Received : 2015.10.23
  • Accepted : 2016.04.25
  • Published : 2016.06.30
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Abstract

The genus Sarcocystis is not usually considered as an important enteric pathogen in immune compromised patients. It might be expected that species for which humans are the final host (Sarcocystis hominis and Sarcocystis suihominis as well as possibly others) would be encountered increasingly often in immunodeficient persons. This study aimed to address how to detect and differentiate Sarcocystis oocysts and/or sporocysts from enteric protozoans in the diarrheal samples of immunodeficient patients in Shiraz, Iran. Diarrheal samples of 741 immunodeficient patients with recurrent persistent or chronic diarrhea were examined by microscopy and molecular biological analysis. Oocysts-positive samples were 68 Cryptosporidium spp., 9 Cystoisospora belli (syn. Isospora belli), 2 Cyclospora cayetanensis, and 15 microsporidia (Enterocytozoon bieneusi). Sarcocystis-like sporocysts found from a woman were identified as Sarcocystis cruzi through 18S rDNA amplification and phylogenetic analysis. To the best of our knowledge, this is the first report of S. cruzi from a human.

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References

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