Particle and aerosol research (한국입자에어로졸학회지)

Korean Association for Particle and Aerosol Research (한국입자에어로졸학회)
권호 표지
DOI QR코드

DOI QR Code

Characteristics of flow field of nose-only exposure chamber for inhalation toxicity evaluation

흡입독성평가를 위한 비부노출 챔버의 유동흐름 특성

  • Noh, Hakjae (Greensolus Co., Ltd.) ;
  • Bong, Choonkeun (Greensolus Co., Ltd.) ;
  • Bong, Hakyung (Greensolus Co., Ltd.) ;
  • Kim, Yonggu (Greensolus Co., Ltd.) ;
  • Cho, Myunghaing (Laboratory of Toxicology, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University) ;
  • Kim, Sanghwa (Laboratory of Toxicology, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University) ;
  • Kim, Daesung (Global Frontier Center for Multiscale Energy Systems, Seoul National University)
  • 노학재 ((주)그린솔루스) ;
  • 봉춘근 ((주)그린솔루스) ;
  • 봉하경 ((주)그린솔루스) ;
  • 김용구 ((주)그린솔루스) ;
  • 조명행 (서울대학교 수의과대학 독성학연구실) ;
  • 김상화 (서울대학교 수의과대학 독성학연구실) ;
  • 김대성 (서울대학교 멀티스케일 에너지 시스템 연구단)
  • Received : 2015.10.30
  • Accepted : 2016.02.12
  • Published : 2016.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

In this work, we evaluated the characteristics of flow field and uniformity of the nose-only exposure chambers for the inhalation toxicity test. Computational fluid dynamics (CFD) modeling was carried out to demonstrate uniformity of the nose-only exposure chambers. Because it is very important in the inhalation toxicity experiments that test materials are distributed uniformly to each holder of the chamber. The test was done with these 3 types of chamber with different form to develop inhalation toxicity evaluation system, easy-to-operate system among exposure chamber used for evaluating inhalation toxicity of environmental chemical mixtures. Through CFD interpretation, nose-only exposure chamber was made with the selection of the optimal conditions. For its evaluation, one type of fragrance was selected and measured particle size distribution of each port. The gene becoming luminous to green fluorescence was combined with GPT-SPE, a type of tGFP vector, to be inhaled to the mouse. Based on this, luminous intensity was checked. As a result, total particle number concentration of each port had average value of $3.17{\times}10^6{\sharp}/cm^3$ and range of the highest and lowest concentration value was approximately ${\pm}4.8%$. Autopsy of lung tissues of mouse showed that it had clearly better delivery of gene compared to the control group.

Keywords

References

  1. A. Afshari, U. Matson, L.E. Ekberg, (2005). Characterization of indoor sources of fine and ultrafine particles: a study conducted in a full‐scale chamber, Indoor air, 15, 141-150. https://doi.org/10.1111/j.1600-0668.2005.00332.x
  2. Jiang HL, Hong SH, Kim YK, Islam MA, Kim HJ, Choi YJ, Nah JW, Lee KH, Han KW, Chae C, Cho CS, Cho MH., (2011). Aerosol delivery of spermine-based poly(amino ester)/Akt1 shRNA complexes for lung cancer gene therapy, Int. J. Pharm.,420(2):256-265. https://doi.org/10.1016/j.ijpharm.2011.08.045
  3. Jun, K.S., (2014). Development and Evaluation of Exposure System for Nano Particle Inhalation Study, Han Yang University Graduate School.
  4. K. Yasuda, M. Tanaka, (2006). Report on hazardous household waste generation in Japan, Waste Management and Research, 24, 397-401. https://doi.org/10.1177/0734242X06064821
  5. Kim, H.M., Kwon, J.T., Kwon, D.Y., Kim, E.J., Lim, Y.M., Rho, H.Y., Kim, P.J., Choi, K.H. (2014). Inhalation toxicity screening study of chemical mixture (I), Risk Assessment Division Environmental Health Research Department National Institute of Environmental Research, 11-1480523-002101-01.
  6. Kim S, Hong SH, Bong CK, Cho MH. (2015). Characterization of air freshener emission: the potential health effects. J Toxicol Sci.,40(5):535-550. https://doi.org/10.2131/jts.40.535
  7. L. Loretz, A.M. Api, L. Barraj, J. Burdick, D.A. Davis, W. Dressler, E. Gilberti, G. Jarrett, S. Mann, Y.H. Laurie Pan, T. Re, K. Renskers, C. Scrafford, S. Vater, (2006). Exposure data for personal care products: Hairspray, spray perfume, liquid foundation, shampoo, body wash, and solid antiperspirant, Food and Chemical Toxicology, 44, 2008-2018. https://doi.org/10.1016/j.fct.2006.06.029
  8. Luu QP, Shin JY, Kim YK, Islam MA, Kang SK, Cho MH, Choi YJ, Cho CS. (2012). High gene transfer by the osmotic polysorbitol-mediated transporter through the selective caveolae endocytic pathway, Mol. Pharm.,9(8):2206-2218. https://doi.org/10.1021/mp300072r
  9. M. Owen, D. Ensor, L. Sparks, (1992). Airborne particle sizes and sources found in indoor air, Atmospheric Environment Part A, 26, 2149-2162. https://doi.org/10.1016/0960-1686(92)90403-8
  10. OECD TG 403, (2009). OECD guideline of the testing of chemicals 403:Acute inhalation toxicity testing, OECD, Paris.
  11. Ostraat, M.L., Swain, K.A., Krajewski, J.J., (2008). $SiO_2$ Aerosol Nano particle Reactor for Occupational Health and Safety Studies, Journal of Occupational and Environmental Hygiene, 5, 390-398. https://doi.org/10.1080/15459620802071646
  12. Phalen, R.F., (1997). Methods in inhalation toxicology, Chapter 5, CRC Press.
  13. Phalen, R.F., (2008). Inhalation studies foundations and techniques, Chapter 5, Informa healthcare.
  14. Yang, W.H., Son, B.S., Yun, C.S., Lee, K.Y., Gwon, E.G., Lim, S.G., Lee, H.S., Lim, C.W., Yun, S.K., Lee, J.D., Lee, C.O., Jo, T.J., Lim, S.G., Kim, J.Y., Park, J.Y., Harm, S.H., Jung, M.H., Her, D., Ser, Y.S. (2009). Studies of personal exposure assessment in accordance with the daily activity patterns of nationals hours, Catholic University of Daegu.