방사선 돌연변이 축엽자소와 야생종 축엽자소 초임계 추출물의 항염증 활성 비교

Comparison of Anti-inflammatory Activity of Extracts with Supercritical Carbon Dioxide from Radiation Mutant Perilla frutescens (L.) Britton and Wild-type

  • 박한철 (한국원자력연구원 첨단방사선연구소) ;
  • 소양강 (한국원자력연구원 첨단방사선연구소) ;
  • 김진백 (한국원자력연구원 첨단방사선연구소) ;
  • 육홍선 (충남대학교 식품영양학과) ;
  • 진창현 (한국원자력연구원 첨단방사선연구소)
  • Park, Hanchul (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • So, Yangkang (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jin-Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Yuk, Hong Sun (Department of Food and Nutrition, Chungnam National University) ;
  • Jin, Chang Hyun (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 투고 : 2016.06.05
  • 심사 : 2016.07.26
  • 발행 : 2016.09.30

초록

In previous study, the radiation mutant Perilla frutescens (L.) Britton with a higher anti-inflammatory activity was selected. The extracts were obtained from the mutant and wild-type using a supercritical carbon dioxide technique. This study aimed to compare the anti-inflammatory activities between the mutant supercritical extract(MSE) and wild-type supercritical extract (WSE). The contents of isoegomaketone (IK) of MSE and WSE were measured through an HPLC analysis. MSE contained IK contents approximately 7-fold higher than those of WSE. To compare the anti-inflammatory activities of MSE and WSE, the expression levels of the mRNA and protein of pro-inflammatory mediators were measured in lipopolysaccharide (LPS)-induced RAW264.7 cells. As a result, MSE inhibited the expression levels of the mRNA and protein of pro-inflammatory mediators, including inducible nitric oxide synthase (iNOS), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and monocyte chemoattractant protein-1 (MCP-1) to a much greater extent than did WSE. Taken together, MSE had more IK contents and higher anti-inflammatory activities than WSE. Therefore, MSE is proposed based on its therapeutic potential in the prevention of inflammatory disease.

키워드

과제정보

이 논문은 2016년도 한국원자력연구원의 연구개발 프로그램과 정부 (미래창조과학부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임 (방사선기술개발사업, No. 2012M2A2A6010575).