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Mband characterization of a cultivar "DanBi 5Ho" with a long shelf life

큰느타리버섯 단핵균주 간 교잡에 의한 저장우수형 품종 "단비5호" 육성 및 특성

  • Lee, Young-Han (Division of Environment-friendly Research, Gyeong sang nam-do Agricultural Research and Extension Services) ;
  • Kim, Ya-El (Division of Environment-friendly Research, Gyeong sang nam-do Agricultural Research and Extension Services) ;
  • Seuk, Su-Won (Division of Environment-friendly Research, Gyeong sang nam-do Agricultural Research and Extension Services) ;
  • Jeong, Jeong-Min (Division of Environment-friendly Research, Gyeong sang nam-do Agricultural Research and Extension Services) ;
  • Ryu, Jae-San (Division of Environment-friendly Research, Gyeong sang nam-do Agricultural Research and Extension Services) ;
  • Heo, Jae-Young (Division of Environment-friendly Research, Gyeong sang nam-do Agricultural Research and Extension Services) ;
  • Kim, Hee-Dae (Division of Environment-friendly Research, Gyeong sang nam-do Agricultural Research and Extension Services) ;
  • Choe, Yong-Jo (Division of Environment-friendly Research, Gyeong sang nam-do Agricultural Research and Extension Services) ;
  • Kim, Min-Keun (Division of Environment-friendly Research, Gyeong sang nam-do Agricultural Research and Extension Services)
  • 이영한 (경상남도농업기술원 친환경연구과) ;
  • 김야엘 (경상남도농업기술원 친환경연구과) ;
  • 석수원 (경상남도농업기술원 친환경연구과) ;
  • 정정민 (경상남도농업기술원 친환경연구과) ;
  • 류재산 (경상남도농업기술원 친환경연구과) ;
  • 허재영 (경상남도농업기술원 친환경연구과) ;
  • 김희대 (경상남도농업기술원 친환경연구과) ;
  • 최용조 (경상남도농업기술원 친환경연구과) ;
  • 김민근 (경상남도농업기술원 친환경연구과)
  • Received : 2016.05.17
  • Accepted : 2016.06.30
  • Published : 2016.06.30

Abstract

Pleurotus eryngii is one of the most widely cultivated and important edible mushrooms. However, the shelf life of the fruiting body is short, which. To solve this problem, a new cultivar, DanBi 5Ho, of Pleurotus eryngii was developed by mono-mono crossing between monokaryotic strains derived from KNR2598 and KNR2610. The optimum temperature formycelial growth was 25 and that forfruiting body development was 15-16. The quality did not change after a period of 40.0 d at 4. This result was significantly different compared to that ofthe control strain Knneuari 2 Ho. Analysis of genetic characteristics of the new hybrid strain DanBi 5Ho revealeda different profile thanthat of the parental and control strains when random amplification of polymorphic DNA (RAPD) primers we used. The results obtained from this study show that DanBi 5Ho is a new hybrid strain, characterized by improved storability after harvesting.

큰느타리버섯 품종 육성을 위해 품질이 우수한 특성을 지니는 큰느타리버섯 KNR2598과 경도가 우수한 특성을 지니는 큰느타리버섯 KNR2610 모본으로부터 단핵균주를 분리 한 뒤 단포자간 교잡을 통해 고품질의 저온 저장성이 우수한 신품종 "단비5호"를 육성하였다. 신품종의 균사 생육 적정온도는 $25^{\circ}C$이며 자실체 발생 적정 온도는 $15{\sim}16^{\circ}C$ 이었다. 솎음 재배에서 발이소요일수를 포함한 수확소요일수는 대조품종인 큰느타리버섯 2호에 비해 0.5~1.7일 정도 빠른 특성을 보였다. 갓 색깔은 중간수준의 갈색이며 대 색깔은 흰색을 나타내었다. 갓모양은 우산형으로 850cc 플라스틱 병재배에서 한 개체의 평균무게는 $90.4{\pm}16.9g$이었다. "단비5호"와 대조품종간의 RAPD 분석결과 서로 다른 DNA 밴드양상을 보여 주었으며 대치배양을 통해 두품종간에 대치선을 확인 하였다. "단비5호"의 경우 $4^{\circ}C$에서의 저장성이 대조품종 대비 24.6~30.6일 이상 길게 유지되는 특성이 있어 수출적합형 품종으로 가치가 있을 것으로 기대된다.

Keywords

References

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