Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Protective Effects of Green Tea Polyphenol Against Renal Injury Through ROS-Mediated JNK-MAPK Pathway in Lead Exposed Rats

  • Wang, Haidong (College of Food and Biological Engineering, Hubei University of Technology) ;
  • Li, Deyuan (Nutrition and Food Research Institute, Wuhan Economic College) ;
  • Hu, Zhongze (Nutrition and Food Research Institute, Wuhan Economic College) ;
  • Zhao, Siming (College of Food Science and Technology, Huazhong Agricultural University) ;
  • Zheng, Zhejun (Nutrition and Food Research Institute, Wuhan Economic College) ;
  • Li, Wei (College of Food and Biological Engineering, Hubei University of Technology)
  • Received : 2015.06.15
  • Accepted : 2016.02.01
  • Published : 2016.06.30
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Abstract

To investigate the potential therapeutic effects of polyphenols in treating Pb induced renal dysfunction and intoxication and to explore the detailed underlying mechanisms. Wistar rats were divided into four groups: control groups (CT), Pb exposure groups (Pb), Pb plus Polyphenols groups (Pb+PP) and Polyphenols groups (PP). Animals were kept for 60 days and sacrificed for tests of urea, serum blood urea nitrogen (BUN) and creatinine. Histological evaluations were then performed. In vitro studies were performed using primary kidney mesangial cells to reveal detailed mechanisms. Cell counting kit-8 (CCK-8) was used to evaluate cell viability. Pb induced cell apoptosis was measured by flow cytometry. Reactive oxygen species (ROS) generation and scavenging were tested by DCFH-DA. Expression level of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), interleukin-1-${\beta}$ (IL-1-${\beta}$) and IL-6 were assayed by ELISA. Western blot and qPCR were used to measure the expression of ERK1/2, JNK1/2 and p38. Polyphenols have obvious protective effects on Pb induced renal dysfunction and intoxication both in vivo and in vitro. Polyphenols reduced Pb concentration and accumulation in kidney. Polyphenols also protected kidney mesangial cells from Pb induced apoptosis. Polyphenols scavenged Pb induced ROS generation and suppressed ROS-mediated ERK/JNK/p38 pathway. Downstream pro-inflammatory cytokines were inhibited in consistency. Polyphenol is protective in Pb induced renal intoxication and inflammatory responses. The underlying mechanisms lie on the antioxidant activity and ROS scavenging activity of polyphenols.

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References

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