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Gadobutrol-dendrimer effects on metastatic and apoptotic gene expression

  • Kebriaezadeh, Abbas (Departments of Toxicology/ Pharmacology and Pharmacoeconomy and Pharmaceutical Management, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Ashrafi, Sepehr (Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Rasouli, Rahimeh (Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Ebrahimi, Seyed Esmaeil Sadat (Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Hamedani, Morteza Pirali (Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Assadi, Artin (Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences) ;
  • Saffari, Mostafa (Islamic Azad University Pharmaceutical Sciences Branch (IAUPS)) ;
  • Ardestani, Mehdi Shafiee (Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences)
  • 투고 : 2016.03.20
  • 심사 : 2016.06.20
  • 발행 : 2016.06.25

초록

Dendrimers are one of the most appropriate nanocaries for imaging moieties in imaging applications.The purpose of this study was the evalution of cytotoxicity and inducing apoptosis of dendrimers. This study was conducted in order to investigate the metastasis suppression effect of dendrimer in human breast MCF-7 cell line and finding the nanoparticle protein corona in biological enviromental. Dendrimer cytotoxicity effect was assessed by MTT assay. The mRNA experession level of KAI1 as a metastasis suppressor gene, Bax as Pro- apoptotic gene, Bcl-2 as an anti-apoptotic gene and GAPDH as a housekepping gene were determined by real-time PCR assays.concentration-dependent nanoparticle cytotoxicity effect was proofed at range of 1-2 mg/mL in 24 hours, significant upregulation of mRNA expression of Bax, was observed whereas expression of anti-apoptotic Bcl-2 was down-regulated, also expression of metastasis suppressor gene KAI1 was up-regulated. So far a few studies confirmed apoptosis enhancement effect of dendrimers in MCF-7 cell line via bax/bcl-2 pathways. dendrimer nanoparticles was able to act as metastase inhibitor via upregulation of KAI1 gene.

키워드

참고문헌

  1. Alavidjeh MS, Haririan I, Khorramizadeh MR, Ghane ZZ, Ardestani MS and Namazi H: (2010), "Anionic linear-globular dendrimers: biocompatible hybrid materials with potential uses in nanomedicine", J. Mater. Sci. Mater. Med., 21, 1121-1133. https://doi.org/10.1007/s10856-009-3978-8
  2. Bengmark, S. (2006), "Curcumin, an atoxic antioxidant and natural NF${\kappa}B$, cyclooxygenase-2, lipooxygenase, and inducible nitric oxide synthase inhibitor: a shield against acute and chronic diseases", J. Parent. Enteral Nutrit., 30, 45-51. https://doi.org/10.1177/014860710603000145
  3. Casey, B.P. and Glazer, P.M. (2001), "Gene targeting via triple-helix formation", Prog. Nucl. Acid Res. Molec. Biol., 67, 163-192. https://doi.org/10.1016/S0079-6603(01)67028-4
  4. D'Emanuele, A. and Attwood, D. (2005), "Dendrimer-drug interactions", Adv. Drug Deliv. Rev., 57, 2147-2162. https://doi.org/10.1016/j.addr.2005.09.012
  5. Darvish Mohamadi, T., Amanlou, M., Ghalandarlaki, N., Mehravi, B., Shafiee Ardestani, M. and Yaghmaei, P. (2013), DTPA-Meglumine-Anionic Linear Globular Dendrimer G1: Novel Nanosized Low Toxic Tumor Molecular MR Imaging Agent, ISRN Pharmaceutics.
  6. Del Pino, P., Pelaz, B., Zhang, Q., Maffre, P., Nienhaus, G.U. and Parak, W.J. (2014), "Protein corona formation around nanoparticles-from the past to the future", Mater. Horiz., 1, 301-313. https://doi.org/10.1039/C3MH00106G
  7. Fox, K.R. and Targeting, D.N.A. (2000), "Targeting DNA with triplexes", Curr. Med. Chem., 7, 17-37. https://doi.org/10.2174/0929867003375506
  8. Haririan, I., Alavidjeh, M.S., Khorramizadeh, M.R., Ardestani, M.S., Ghane, Z.Z. and Namazi, H. (2010), "Anionic linear-globular dendrimer-cis-platinum (II) conjugates promote cytotoxicity in vitro against different cancer cell lines", Int. J. Nanomed., 5, 63.
  9. Jain, K., Kesharwani, P., Gupta, U. and Jain, N. (2010), "Dendrimer toxicity: let's meet the challenge", Int. J. Pharmaceut., 394, 122-142. https://doi.org/10.1016/j.ijpharm.2010.04.027
  10. Khosroshahi, A., Amanlou, M., Sabzevari, O., Daha, F., Aghasadeghi, M., Ghorbani, M., Ardestani, M., Alavidjeh, M., Sadat, S. and Pouriayevali, M. (2013), "A comparative study of two novel nanosized radiolabeled analogues of methionine for SPECT tumor imaging", Curr. Med. Chem., 20, 123-133.
  11. Mahmoudi, M., Lohse, S.E., Murphy, C.J., Fathizadeh, A., Montazeri, A. and Suslick, K.S. (2013), "Variation of protein corona composition of gold nanoparticles following plasmonic heating", Nano Lett., 14, 6-12.
  12. Medina, S.H. and El-Sayed, M.E. (2009), "Dendrimers as carriers for delivery of chemotherapeutic agents", Chem. Rev., 109, 3141-3157. https://doi.org/10.1021/cr900174j
  13. Mirshafiee, V., Kim, R., Park, S., Mahmoudi, M. and Kraft, M.L. (2016), "Impact of protein pre-coating on the protein corona composition and nanoparticle cellular uptake", Biomater., 75, 295-304. https://doi.org/10.1016/j.biomaterials.2015.10.019
  14. Mohammadi, E., Amanlou, M., Ebrahimi, S.E.S., Hamedani, M.P., Mahrooz, A., Mehravi, B., Emami, B.A., Aghasadeghi, M.R., Bitarafan-Rajabi, A. and Akbar, H.R.P.A. (2014), "Cellular uptake, imaging and pathotoxicological studies of a novel Gd [iii]-DO3A-butrol nano-formulation", RSC Adv., 4, 45984-45994. https://doi.org/10.1039/C4RA05596A
  15. Pryor, J.B., Harper, B.J. and Harper, S.L. (2014), "Comparative toxicological assessment of PAMAM and thiophosphoryl dendrimers using embryonic zebrafish", Int. J. Nanomed., 9, 1947.
  16. Serpooshan, V., Mahmoudi, M., Zhao, M., Wei, K., Sivanesan, S., Motamedchaboki, K., Malkovskiy, A.V., Goldstone, A.B., Cohen, J.E. and Yang, P.C. (2015), "Protein corona influences cell-biomaterial interactions in nanostructured tissue engineering scaffolds", Adv. Funct. Mater., 25, 4379-4389. https://doi.org/10.1002/adfm.201500875
  17. Sharma, R., Gescher, A. and Steward, W. (2005), "Curcumin: the story so far", Eur. J. Cancer, 41, 1955-1968. https://doi.org/10.1016/j.ejca.2005.05.009
  18. Ye, Z., Guntaka, R.V. and Mahato, R.I. (2007), "Sequence-specific triple helix formation with genomic DNA", Biochemistry, 46, 11240-11252. https://doi.org/10.1021/bi700580y

피인용 문헌

  1. Nanofibers for Biomedical and Healthcare Applications pp.16165187, 2018, https://doi.org/10.1002/mabi.201800256