Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effect of Germanium Treatment on Growth and Production of Organic Germanium in Oplopanax elatus

게르마늄 처리에 따른 땃두릅나무의 생육 증진 효과 및 유기게르마늄 생산

  • Kim, Hee Young (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Seong, Eun Soo (Department of Medicinal Plant, Suwon Women's University) ;
  • Yoo, Ji Hye (Bioherb Research Institute, Kangwon National University) ;
  • Choi, Jae Hoo (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Kang, Byeong Ju (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Jeon, Mi Ran (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Kim, Myong Jo (Department of Bio-Resource Sciences, Kangwon National University) ;
  • Yu, Chang Yeon (Department of Bio-Resource Sciences, Kangwon National University)
  • 김희영 (강원대학교 생물자원과학과) ;
  • 성은수 (수원여자대학교 약용식물과) ;
  • 유지혜 (강원대학교 한방바이오연구소) ;
  • 최재후 (강원대학교 생물자원과학과) ;
  • 강병주 (강원대학교 생물자원과학과) ;
  • 전미란 (강원대학교 생물자원과학과) ;
  • 김명조 (강원대학교 생물자원과학과) ;
  • 유창연 (강원대학교 생물자원과학과)
  • Received : 2016.03.31
  • Accepted : 2016.06.08
  • Published : 2016.06.30
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Abstract

Background: This study was conducted to investigate the effects of germanium treatment on the growth and organic germanium production in the roots of Oplopanax elatus plantlets. Methods and Results: O. elatus plantlets were cultured in Murashige and Skoog (MS) medium with different concentrations of germanium dioxide ($GeO_2$) to analyze optimum growth conditions. Exogenous treatment of $10mg/{\ell}\;GeO_2$ promoted growth and an increase in the contents of chlorophyll a, b and carotenoid in O. elatus. The germanium accumulation and production in roots of O. elatus plantlets treated with organic germanium reached the highest levels. The growth of the aerial and underground portion of O. elatus with organic germanium was greater than that of the control. The accumulation and production of organic germanium reached the highest level ($40.89{\mu}g/plantlet$) with the treatment of $50mg/{\ell}\;GeO_2$. Antioxidant activity measured by DPPH and ABTS assays also increased with the germanium treatment and improved the DPPH and ABTS radical activity by 200% compared with that in the control. In addition, the total phenol and flavonoid contents of the plantlets with a treatment of $50mg/{\ell}\;GeO_2$ were higher than in the control. Conclusions: Taken together, the growth of O. elatus was increased with the treatment of $50mg/{\ell}\;GeO_2$ germanium and the biological references improved, with increased antioxidant activity and organic germanium production.

Keywords

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