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산지별 고려엉겅퀴의 Pectolinarin 함량 및 항산화 활성

Analysis of Pectolinarin Content and Antioxidant activity in Cirsium setidens Nakai by Cultivars

  • Cho, Bong-Yeon (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Jin-Ha (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Ra, Moon-Jin (Hongcheon Institute of Medicinal Herb) ;
  • Kim, Sun-Young (Hongcheon Institute of Medicinal Herb) ;
  • Kang, Il-Jun (Department of Food Science and Nutrition, Hallym University) ;
  • Han, Kyoung-Chan (HATTI Co., Ltd.) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
  • 투고 : 2016.03.30
  • 심사 : 2016.04.12
  • 발행 : 2016.06.30

초록

본 연구에서는 국내산 고려엉겅퀴를 건강기능식품 소재로 활용시 기초자료 제공하고자 주요 산지별 pectolinarin 함량, 총 플라보노이드 함량, DPPH라디칼 소거능 및 ORAC 지수 등을 분석하였다. 산지별 고려엉겅퀴의 pectolinarin 함량은 $3.95{\pm}0.05{\sim}7.29{\pm}0.07mg/g$로 강원도 D농장(GW-D)에서 가장 높은 함량을 보였다. 총 플라보노이드 함량은 산지별로 $40.42{\pm}0.91{\sim}76.70{\pm}2.24mg\;PNE/g$ 수준으로 전라남도 A농장(JN-A) 추출물에서 가장 높은 함량을 나타내었고 강원도 A농장(GW-A) 추출물에서($40.42{\pm}0.91mg\;PNE/g$)로 가장 낮은 함량을 보였다. DPPH 라디칼 소거능의 경우, 산지별로 31.25% ~ 81.93%의 활성을 보였고, ORAC 지수는 산지별로 $179.62{\sim}514.49{\mu}M\;TE/g$로 강원도 D농장(GW-D)과 강원도 E농장(GW-E)에서 각각 514.49, $501.73{\mu}M\;TE/g$ 으로 가장 높은 ORAC 수치를 나타내었다. 결론적으로 고려엉겅퀴 추출물은 pectolinarin과 유용성분을 함유하고 DPPH 라디칼 소거능 및 ORAC 활성 등의 항산화활성을 갖는 것으로 나타났다.

This study was performed to provide basic data of Cirsium setidens Nakai by cultivars that will be applied for development of functional foods and ingredients. We assessed pectiolinarin content, total flavonoids content and antioxidant effects (DPPH radical scavenging activity and ORAC assay) of C. setidens Nakai. Our results showed that the pectolinarin and total flavonoids contents of C. setidens Nakai by cultivars ranged from $3.95{\pm}0.05$ to $7.29{\pm}0.07mg/g$ and from $40.42{\pm}0.91$ to $76.70{\pm}2.24mg$ pectolinarin equivalent (PNE)/g, respectively. Among C. setidens Nakai by cultivars, the pectolinarin content was highest in GW-D extract. Futhermore, the DPPH radical scavenging activity of C. setidens Nakai ranged from 31.25 to 81.93%, respectively. The oxygen radical absorbance capacity (ORAC) value was highest in GW-D and GW-E extracts (514.49 and $501.73{\mu}M\;TE/g$, respectively). These results suggest that C. setidens Nakai extract could be considered as a good sources of natural antioxidants and functional food ingredients.

키워드

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