Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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In vitro shoot regeneration and genetic transformation of the gerbera (Gerbera hybrida Hort.) cultivar 'Gold Eye'

  • Chung, Mi-Young (Department of Agricultural Education, Sunchon National University) ;
  • Kim, Min Bae (Department of Agricultural Education, Sunchon National University) ;
  • Chung, Yong Mo (Flower Research Institute, Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Nou, Ill-Sup (Department of Horticulture, Sunchon National University) ;
  • Kim, Chang Kil (Department of Horticultural Science, Kyungpook National University)
  • Received : 2016.05.31
  • Accepted : 2016.06.13
  • Published : 2016.06.30
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Abstract

This research was conducted to improve the cold tolerance of the gerbera cv. Gold Eye by introduction of the Arabidopsis $Ca^{2+}/H^+$ antiporter gene (CAX1) via Agrobacterium-mediated transformation. Prior to genetic transformation, we optimized a combination of plant growth regulators; $1.0mgl^{-1}$ 6-Benzyladenine (BA) and $0.1mgl^{-1}$3-indole-acetic acid (IAA) were found to lead to proper in vitro shoot regeneration from petiole explants. In addition, $50mgl^{-1}$ kanamycin was determined to be the minimal concentration useful for selection of putative transgenic plants. In this study, transgenic gerbera expressing the Arabidopsis $Ca^{2+}/H^+$ antiporter gene (CAX1) were obtained using the optimized concentrations. We expect that introduction of the gene to the cultivar will improve cold tolerance, which will be important in the winter months.

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References

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